EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.11 | sucrose | the production of isomaltulose with alginate-immobilized Protaminobacter rubrum at 30 °C is influenced by sucrose concentration. Isomaltulose yields above 90% in 8 h are reached in 40-65% sucrose solutions, and yields up to 82% in 24 h were obtained with 70% sucrose. At higher concentrations (75%) a significant decrease in the isomaltulose yield (42.7-55%) is observed | Serratia plymuthica |
EC Number | General Stability | Organism |
---|---|---|
5.4.99.11 | the isomaltulose synthase immobilized only with alginate remains stable for eight 24 h batch cycles or 192 h total time | Serratia plymuthica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.11 | Glutaraldehyde | the application of 1 or 2% glutaraldehyde after immobilization of Protaminobacter rubrum in alginate results in the complete destruction of the isomaltulose synthase activity | Serratia plymuthica |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.11 | Serratia plymuthica | - |
- |
- |
5.4.99.11 | Serratia plymuthica CBS 574.77 | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.4.99.11 | additional information | - |
the specific activity of the Protaminobacter rubrum immobilized pellets in calcium alginate at 30°C range from 1.6 to 4.0 g isomaltulose/g pellet/h, respectively with 70% and 65% sucrose solution, while in lower sucrose concentration have higher specific activities presumably due to substrate inhibition of the isomaltulose synthase in higher sucrose concentrations | Serratia plymuthica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.11 | sucrose | - |
Serratia plymuthica | isomaltulose | 89-94% yield | ? | |
5.4.99.11 | sucrose | - |
Serratia plymuthica CBS 574.77 | isomaltulose | 89-94% yield | ? |