Literature summary extracted from
Bharatham, N.; Bharatham, K.; Lee, Y.; Woo Lee, K.
Molecular dynamics simulation study of valyl-tRNA synthetase with its pre- and post-transfer editing substrates (2009), Biophys. Chem., 143, 34-43.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
6.1.1.9 |
expression in Escherichia coli |
Thermus thermophilus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
6.1.1.9 |
molecular dynamics simulation studies based on PDB structure ID 1wk9. Noncognate substrates Thr-AMP and Thr-A76, bind more strongly than the cognate substrates Val-AMP and Val-A76 in both pre- and post-transfer editing, respectivel |
Thermus thermophilus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
6.1.1.9 |
D279A |
editing site mutation, severely affects the binding ability of pre-transfer substrate Thr-AMP |
Thermus thermophilus |
6.1.1.9 |
K270A |
editing site mutation, severely affects the binding ability of pre-transfer substrate Thr-AMP |
Thermus thermophilus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
6.1.1.9 |
Thermus thermophilus |
P96142 |
- |
- |