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Literature summary extracted from

  • Krishnan, K.; Prathiba, K.; Jayaprakash, V.; Basu, A.; Mishra, N.; Zhou, B.; Hu, S.; Yen, Y.
    Synthesis and ribonucleotide reductase inhibitory activity of thiosemicarbazones (2008), Bioorg. Med. Chem. Lett., 18, 6248-6250.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 ATP
-
 Homo sapiens

Application

EC Number Application Comment Organism
1.17.4.1 drug development the enzyme is an important therapeutic target for anticancer drugs Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.4.1 expression of His6-tagged subunits hRRM1 and hRRM2 in Escherichia coli strain BL21(DE3) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.1 2-furan-3-ylbenzaldehyde N-(4-hydroxyphenyl)thiosemicarbazone
-
 Homo sapiens
1.17.4.1 2-furan-3-ylbenzaldehyde N-phenylthiosemicarbazone
-
 Homo sapiens
1.17.4.1 2-hydroxybenzaldehyde N-(4-chlorophenyl)thiosemicarbazone
-
 Homo sapiens
1.17.4.1 2-hydroxybenzaldehyde N-phenylthiosemicarbazone
-
 Homo sapiens
1.17.4.1 2-thiophen-2-ylbenzaldehyde N-(4-chlorophenyl)thiosemicarbazone
-
 Homo sapiens
1.17.4.1 2-thiophen-2-ylbenzaldehyde N-phenylthiosemicarbazone
-
 Homo sapiens
1.17.4.1 4-hydroxy-3-methoxybenzaldehyde N-(4-chlorophenyl)thiosemicarbazone
-
 Homo sapiens
1.17.4.1 4-hydroxy-3-methoxybenzaldehyde N-phenylthiosemicarbazone
-
 Homo sapiens
1.17.4.1 4-hydroxybenzaldehyde N-(2-chlorophenyl)thiosemicarbazone
-
 Homo sapiens
1.17.4.1 4-hydroxybenzaldehyde N-(2-hydroxyphenyl)thiosemicarbazone
-
 Homo sapiens
1.17.4.1 4-hydroxybenzaldehyde N-(2-methoxyphenyl)thiosemicarbazone
-
 Homo sapiens
1.17.4.1 4-hydroxybenzaldehyde N-(2-methylphenyl)thiosemicarbazone
-
 Homo sapiens
1.17.4.1 4-hydroxybenzaldehyde N-(2-nitrophenyl)thiosemicarbazone
-
 Homo sapiens
1.17.4.1 4-hydroxybenzaldehyde N-(3-chlorophenyl)thiosemicarbazone
-
 Homo sapiens
1.17.4.1 4-hydroxybenzaldehyde N-(3-hydroxyphenyl)thiosemicarbazone
-
 Homo sapiens
1.17.4.1 4-hydroxybenzaldehyde N-(3-methylphenyl)thiosemicarbazone
-
 Homo sapiens
1.17.4.1 4-hydroxybenzaldehyde N-(4-chlorophenyl)thiosemicarbazone
-
 Homo sapiens
1.17.4.1 4-hydroxybenzaldehyde N-(4-hydroxyphenyl)thiosemicarbazone
-
 Homo sapiens
1.17.4.1 4-hydroxybenzaldehyde N-(4-methylphenyl)thiosemicarbazone
-
 Homo sapiens
1.17.4.1 4-hydroxybenzaldehyde N-(4-nitrophenyl)thiosemicarbazone
-
 Homo sapiens
1.17.4.1 4-hydroxybenzaldehyde N-phenylthiosemicarbazone
-
 Homo sapiens
1.17.4.1 Hydroxyurea
-
 Homo sapiens
1.17.4.1 additional information synthesis and ribonucleotide reductase inhibitory activity of thiosemicarbazones, overview Homo sapiens
1.17.4.1 triapine
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.1 Mg2+
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.1 recombinant His6-tagged subunits hRRM1 and hRRM2 from Escherichia coli strain BL21(DE3) Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 additional information CDP as substrate resulting information of product dCDP Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.4.1 tetramer 1:1 complex of two homodimeric subunits, hRRM1 and hRRM2 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.17.4.1 ribonucleotide reductase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.4.1 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.4.1 7.2
-
 assay at Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.17.4.1 0.014
-
 pH 7.2, 37°C Homo sapiens 4-hydroxybenzaldehyde N-(4-methylphenyl)thiosemicarbazone
1.17.4.1 0.029
-
 pH 7.2, 37°C Homo sapiens 4-hydroxybenzaldehyde N-(3-chlorophenyl)thiosemicarbazone
1.17.4.1 0.029
-
 pH 7.2, 37°C Homo sapiens 4-hydroxybenzaldehyde N-(2-hydroxyphenyl)thiosemicarbazone
1.17.4.1 0.029
-
 pH 7.2, 37°C Homo sapiens 4-hydroxybenzaldehyde N-(3-hydroxyphenyl)thiosemicarbazone
1.17.4.1 0.03
-
 pH 7.2, 37°C Homo sapiens 4-hydroxybenzaldehyde N-(3-methylphenyl)thiosemicarbazone
1.17.4.1 0.037
-
 pH 7.2, 37°C Homo sapiens 4-hydroxybenzaldehyde N-(2-methylphenyl)thiosemicarbazone
1.17.4.1 0.039
-
 pH 7.2, 37°C Homo sapiens 4-hydroxybenzaldehyde N-(2-nitrophenyl)thiosemicarbazone
1.17.4.1 0.042
-
 above, pH 7.2, 37°C Homo sapiens 4-hydroxybenzaldehyde N-(2-methoxyphenyl)thiosemicarbazone
1.17.4.1 0.043
-
 pH 7.2, 37°C Homo sapiens 4-hydroxybenzaldehyde N-(4-hydroxyphenyl)thiosemicarbazone
1.17.4.1 0.045
-
 pH 7.2, 37°C Homo sapiens 4-hydroxybenzaldehyde N-(4-nitrophenyl)thiosemicarbazone
1.17.4.1 0.047
-
 pH 7.2, 37°C Homo sapiens 4-hydroxybenzaldehyde N-(2-chlorophenyl)thiosemicarbazone
1.17.4.1 0.242
-
 pH 7.2, 37°C Homo sapiens 4-hydroxybenzaldehyde N-phenylthiosemicarbazone
1.17.4.1 0.287
-
 pH 7.2, 37°C Homo sapiens 4-hydroxybenzaldehyde N-(4-chlorophenyl)thiosemicarbazone
1.17.4.1 0.5
-
 above, pH 7.2, 37°C Homo sapiens 2-hydroxybenzaldehyde N-phenylthiosemicarbazone
1.17.4.1 0.5
-
 above, pH 7.2, 37°C Homo sapiens 2-hydroxybenzaldehyde N-(4-chlorophenyl)thiosemicarbazone
1.17.4.1 0.5
-
 above, pH 7.2, 37°C Homo sapiens 4-hydroxy-3-methoxybenzaldehyde N-phenylthiosemicarbazone
1.17.4.1 0.5
-
 above, pH 7.2, 37°C Homo sapiens 4-hydroxy-3-methoxybenzaldehyde N-(4-chlorophenyl)thiosemicarbazone
1.17.4.1 0.5
-
 above, pH 7.2, 37°C Homo sapiens 2-furan-3-ylbenzaldehyde N-phenylthiosemicarbazone
1.17.4.1 0.5
-
 above, pH 7.2, 37°C Homo sapiens 2-furan-3-ylbenzaldehyde N-(4-hydroxyphenyl)thiosemicarbazone
1.17.4.1 0.5
-
 above, pH 7.2, 37°C Homo sapiens 2-thiophen-2-ylbenzaldehyde N-phenylthiosemicarbazone
1.17.4.1 0.5
-
 above, pH 7.2, 37°C Homo sapiens 2-thiophen-2-ylbenzaldehyde N-(4-chlorophenyl)thiosemicarbazone