Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gvozdev, R.; Tukhvatullin, I.; Tumanova, L.
    Purification and properties of membrane-bound methane hydroxylase from Methylococcus capsulatus (Strain M) (2008), Biol. Bull., 35, 161-169.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.18.3 additional information the active center of pMH is located in the beta-subunit Methylococcus capsulatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.3 Acetylene is a competitive inhibitor of pMH, interacts with its substrate-binding center Methylococcus capsulatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.3 membrane
-
 Methylococcus capsulatus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.3 Cu2+ pMH contains 2-4 atoms of copper per a minimum molecular weight of 99 kDa Methylococcus capsulatus
1.14.18.3 Iron pMH contains 1 or 2 atoms of nonheme iron Methylococcus capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.3 25000
-
 pMH, 1 * 47000, + 1 * 27000 + 1 * 25000, alphabetagamma-subunit, SDS-PAGE Methylococcus capsulatus
1.14.18.3 27000
-
 pMH, 1 * 47000, + 1 * 27000 + 1 * 25000, alphabetagamma-subunit, SDS-PAGE Methylococcus capsulatus
1.14.18.3 47000
-
 pMH, 1 * 47000, + 1 * 27000 + 1 * 25000, alphabetagamma-subunit, SDS-PAGE Methylococcus capsulatus
1.14.18.3 99000
-
-
 Methylococcus capsulatus

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.3 Methylococcus capsulatus
-
-
-
1.14.18.3 Methylococcus capsulatus M
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.3 particulate form of methane hydroxylase (pMH) obtained by ion exchange and hydrophobic chromatography Methylococcus capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.3 methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus methanol + acceptor + H2O
-
 ?
1.14.18.3 methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus M methanol + acceptor + H2O
-
 ?
1.14.18.3 Mn2+ + H2O2
-
 Methylococcus capsulatus ?
-
 ?
1.14.18.3 Mn2+ + H2O2
-
 Methylococcus capsulatus M ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.3 heterotrimer pMH, 1 * 47000, + 1 * 27000 + 1 * 25000, alphabetagamma-subunit, SDS-PAGE Methylococcus capsulatus

Synonyms

EC Number Synonyms Comment Organism
1.14.18.3 membrane-bound methane monooxygenase
-
 Methylococcus capsulatus
1.14.18.3 methane hydroxylase
-
 Methylococcus capsulatus
1.14.18.3 PMH
-
 Methylococcus capsulatus
1.14.18.3 pMMO
-
 Methylococcus capsulatus