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Literature summary extracted from
- Rational design of Bacillus stearothermophilus US100 L-arabinose isomerase: potential applications for D-tagatose production (2009), Biochimie, 91, 650-653.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 5.3.1.4 | synthesis | engineered enzyme mutants are useful for production of D-tagatose | Geobacillus stearothermophilus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.4 | expression of wild-type and mutant enzymes in Escherichia coli strain HB101 | Geobacillus stearothermophilus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.3.1.4 | additional information | construction of three Bacillus stearothermophilus US100 L-arabinose isomerase mutants with increased activity for D-galactose and increased D-tagatose production | Geobacillus stearothermophilus |
| 5.3.1.4 | N175H | site-directed mutagenesis, the N175H mutant has a broad optimal temperature range from 50 to 65°C | Geobacillus stearothermophilus |
| 5.3.1.4 | Q268K | site-directed mutagenesis, the mutant enzyme shows increased acidotolerance and is more stable at acidic pH than the wild-type enzyme | Geobacillus stearothermophilus |
| 5.3.1.4 | Q268K/N175H | site-directed mutagenesis, the Q268K mutant is more acidotolerant, the N175H mutant has a broad optimal temperature range from 50 to 65°C | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.4 | L-arabinose | Geobacillus stearothermophilus | - |
L-ribulose | - |
? |  |
| 5.3.1.4 | L-arabinose | Geobacillus stearothermophilus US100 | - |
L-ribulose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.4 | Geobacillus stearothermophilus | Q9S467 | - |
- |
| 5.3.1.4 | Geobacillus stearothermophilus US100 | Q9S467 | - |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | additional information | - |
D-galactose bioconversion rates in% at different pH values at 65°C and 70°C with wild-type and mutant enzymes, overview | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.4 | D-galactose | - |
Geobacillus stearothermophilus | D-tagatose | - |
? | |
| 5.3.1.4 | D-galactose | - |
Geobacillus stearothermophilus US100 | D-tagatose | - |
? | |
| 5.3.1.4 | L-arabinose | - |
Geobacillus stearothermophilus | L-ribulose | - |
? | |
| 5.3.1.4 | L-arabinose | - |
Geobacillus stearothermophilus US100 | L-ribulose | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 50 | 65 | mutant Q268K/N175H, stable without addition of metal ions | Geobacillus stearothermophilus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 30 | 90 | - |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 65 | - |
pH 7.5, wild-type enzyme: half-life 70 h, mutant Q268K: half-life 69 h, mutant N175H: half-life 70 h, mutant Q268K/N175H: half-life 71 h | Geobacillus stearothermophilus |
| 5.3.1.4 | 70 | - |
pH 7.5, wild-type enzyme: half-life 1.8 h, mutant Q268K: half-life 1.6 h, mutant N175H: half-life 0.7 h, mutant Q268K/N175H: half-life 0.6 h | Geobacillus stearothermophilus |
| 5.3.1.4 | 75 | - |
pH 7.5, wild-type enzyme: half-life 1.0 h, mutant Q268K: half-life 0.9 h, mutant N175H: half-life 0.4 h, mutant Q268K/N175H: half-life 0.4 h | Geobacillus stearothermophilus |
| 5.3.1.4 | 80 | - |
pH 7.5, wild-type enzyme: half-life 0.2 h, mutant Q268K: half-life 0.2 h, mutants N175H and Q268K/N175H are inactivated | Geobacillus stearothermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 6 | 7 | mutant Q268K/N175H, stable without addition of metal ions | Geobacillus stearothermophilus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 5 | 9 | - |
Geobacillus stearothermophilus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 5 | - |
65°C, wild-type enzyme: half-life 13 h, mutant Q268K: half-life 43 h, mutant N175H: half-life 13 h, mutant Q268K/N175H: half-life 43 h | Geobacillus stearothermophilus |
| 5.3.1.4 | 7.5 | - |
65°C, wild-type enzyme: half-life 69 h, mutant Q268K: half-life 65 h, mutant N175H: half-life 69 h, mutant Q268K/N175H: half-life 64 h | Geobacillus stearothermophilus |
| 5.3.1.4 | 8.5 | - |
65°C, wild-type enzyme: half-life 51 h, mutant Q268K: half-life 38 h, mutant N175H: half-life 50 h, mutant Q268K/N175H: half-life 37 h | Geobacillus stearothermophilus |
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