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Literature summary extracted from

  • Ma, Q.; Xu, Y.
    Characterization of a caffeic acid 3-O-methyltransferase from wheat and its function in lignin biosynthesis (2008), Biochimie, 90, 515-524.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.68 expressed in Escherichia coli BL21 cells and in Nicotiana tabacum cultivar Wisconsin 38 Triticum aestivum
2.1.1.104 DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Triticum aestivum
2.1.1.104 recombinant expression in transgenic plants using the transformation by Agrobacterium tumefaciens strain LBA4404 Nicotiana tabacum

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.104 additional information construction of transgenic COMT knockout Nicotiana tabacum plants by antisense expression of COMT from Triticum aestivum via Agrobacterium tumefaciens transfection, phenotype, overview Triticum aestivum
2.1.1.104 additional information construction of transgenic COMT knockout Nicotiana tabacum plants by antisense expression of COMT from Triticum aestivum, phenotype, overview Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.68 0.01731
-
 5-hydroxyconiferaldehyde
-
 Triticum aestivum
2.1.1.68 0.04372
-
 caffeoyl aldehyde
-
 Triticum aestivum
2.1.1.68 0.06875
-
 3,4-dihydroxy-trans-cinnamate
-
 Triticum aestivum
2.1.1.68 0.08403
-
 caffeoyl alcohol
-
 Triticum aestivum
2.1.1.68 0.1002
-
 5-hydroxyconiferyl alcohol
-
 Triticum aestivum
2.1.1.104 0.0173
-
 5-hydroxy-coniferaldehyde
-
 Triticum aestivum
2.1.1.104 0.0437
-
 caffeoyl aldehyde
-
 Triticum aestivum
2.1.1.104 0.0688
-
 caffeic acid
-
 Triticum aestivum
2.1.1.104 0.083
-
 caffeoyl-CoA
-
 Triticum aestivum
2.1.1.104 0.0952
-
 5-hydroxy-feruloyl-CoA
-
 Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.68 40000
-
 SDS-PAGE Triticum aestivum
2.1.1.104 38600
-
 x * 38600, about, sequence calculation Triticum aestivum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.104 additional information Triticum aestivum COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway ?
-
 ?
2.1.1.104 additional information Nicotiana tabacum COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA Triticum aestivum
-
 S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.68 Triticum aestivum Q38J50 cultivar H4564
-
2.1.1.104 Nicotiana tabacum O24149 cv. Wisconsin 38
-
2.1.1.104 Triticum aestivum Q38J50 cv. H4564
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.68 Ni-NTA column chromatography Triticum aestivum
2.1.1.104 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, expression of antisense enzyme in transgenic Nicotiana tabacum plants Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.68 leaf
-
 Triticum aestivum
-
2.1.1.68 root
-
 Triticum aestivum
-
2.1.1.68 stem
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.104 additional information
-
 determination of lignin content in transgenic plants, overview Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.68 additional information the enzyme exhibits also a strong activity towards ester precursors including caffeoyl-CoA and 5-hydroxyferuloyl-CoA Triticum aestivum ?
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
-
 Triticum aestivum S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde exhibits the highest catalyzing efficiency towards 5-hydroxyconiferaldehyde Triticum aestivum S-adenosyl-L-homocysteine + 5-methoxyconiferaldehyde
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyconiferyl alcohol
-
 Triticum aestivum S-adenosyl-L-homocysteine + 5-hydroxy-3-methoxyconiferyl alcohol
-
 ?
2.1.1.68 S-adenosyl-L-methionine + caffeoyl alcohol
-
 Triticum aestivum S-adenosyl-L-homocysteine + 4-[(1E)-3-hydroxyprop-1-en-1-yl]-2-methoxyphenol
-
 ?
2.1.1.68 S-adenosyl-L-methionine + caffeoyl aldehyde exhibits the highest catalyzing efficiency towards caffeoyl aldehyde Triticum aestivum S-adenosyl-L-homocysteine + coniferyl aldehyde
-
 ?
2.1.1.104 additional information COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway Triticum aestivum ?
-
 ?
2.1.1.104 additional information COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway Nicotiana tabacum ?
-
 ?
2.1.1.104 additional information the enzyme is capable of 3'- and 5'-methylating not only acid, aldehyde and alcohol precursors, but also ester precursors, broad substrate specificity, overview Triticum aestivum ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA
-
 Triticum aestivum S-adenosyl-L-homocysteine + sinapoyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
-
 Triticum aestivum S-adenosyl-L-homocysteine + sinapaldehyde
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeic acid
-
 Triticum aestivum S-adenosyl-L-homocysteine + ferulic acid
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl alcohol
-
 Triticum aestivum S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl aldehyde
-
 Triticum aestivum S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA
-
 Triticum aestivum S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.104 ? x * 38600, about, sequence calculation Triticum aestivum

Synonyms

EC Number Synonyms Comment Organism
2.1.1.68 caffeic acid 3-O-methyltransferase
-
 Triticum aestivum
2.1.1.68 COMT
-
 Triticum aestivum
2.1.1.104 caffeic acid 3-O-methyltransferase
-
 Triticum aestivum
2.1.1.104 caffeic acid 3-O-methyltransferase
-
 Nicotiana tabacum
2.1.1.104 COMT
-
 Triticum aestivum
2.1.1.104 COMT
-
 Nicotiana tabacum

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.104 S-adenosyl-L-methionine
-
 Triticum aestivum

General Information

EC Number General Information Comment Organism
2.1.1.104 metabolism COMT plays a pivotal position in the lignin biosynthetic pathway Triticum aestivum
2.1.1.104 metabolism COMT plays a pivotal position in the lignin biosynthetic pathway Nicotiana tabacum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.68 0.0011
-
 5-hydroxyconiferyl alcohol
-
 Triticum aestivum
2.1.1.68 0.0014
-
 3,4-dihydroxy-trans-cinnamate
-
 Triticum aestivum
2.1.1.68 0.0017
-
 caffeoyl alcohol
-
 Triticum aestivum
2.1.1.68 0.0024
-
 caffeoyl aldehyde
-
 Triticum aestivum
2.1.1.68 0.0037
-
 5-hydroxyconiferaldehyde
-
 Triticum aestivum