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Literature summary extracted from
- Catalytic role of a conserved cysteine residue in the desulfonation reaction by the alkanesulfonate monooxygenase enzyme (2010), Biochim. Biophys. Acta, 1804, 97-105.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.5 | ligated into pET21a plasmid containing the ssuD gene, wild-type and mutants expressed in Escherichia coli BL21(DE3) super-competent cells | Escherichia coli |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.14.5 | C54A | has little effect on FMN or FMNH2 binding, kcat/Km value decreases 6fold relative to wild-type | Escherichia coli |
| 1.14.14.5 | C54S | has little effect on FMN or FMNH2 binding, kcat/Km value increases 3fold relative to wild-type. Is able to generate the C4a-(hydro)peroxyflavin, but the rate of formation is increased 10fold relative to wild-type | Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.5 | 0.0075 | - |
octanesulfonate | mutant C54S | Escherichia coli |  |
| 1.14.14.5 | 0.0264 | - |
octanesulfonate | mutant C54A | Escherichia coli | |
| 1.14.14.5 | 0.044 | - |
octanesulfonate | wild-type SsuD | Escherichia coli | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.5 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.5 | - |
Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.14.5 | -80°C | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.5 | additional information | Cys54 in SsuD may be either directly or indirectly involved in stabilizing the C4a-(hydro)peroxyflavin intermediate formed during catalysis through hydrogen bonding interactions | Escherichia coli | ? | - |
? | |
| 1.14.14.5 | octanesulfonate + FMNH2 + O2 | - |
Escherichia coli | octanal + FMN + sulfite + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.5 | SsuD | - |
Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.5 | 0.093 | - |
octanesulfonate | mutant C54A | Escherichia coli | |
| 1.14.14.5 | 0.47 | - |
octanesulfonate | mutant C54S | Escherichia coli | |
| 1.14.14.5 | 0.86 | - |
octanesulfonate | wild-type SsuD | Escherichia coli | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.5 | FMN | - |
Escherichia coli | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.5 | 3.3 | - |
octanesulfonate | mutant C54A | Escherichia coli | |
| 1.14.14.5 | 20 | - |
octanesulfonate | wild-type SsuD | Escherichia coli | |
| 1.14.14.5 | 63.3 | - |
octanesulfonate | mutant C54S | Escherichia coli | |
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Release 2023.1 (January 2023)
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