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Literature summary extracted from

  • Shokeer, A.; Mannervik, B.
    Residue 234 is a master switch of the alternative-substrate activity profile of human and rodent theta class glutathione transferase T1-1 (2010), Biochim. Biophys. Acta, 1800, 466-473.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain XL1-Blue Mus musculus
2.5.1.18 expression of His-tagged wild-type enzyme in Escherichia coli strain XL1-Blue Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.18 R234W site-directed mutagenesis Mus musculus
2.5.1.18 W234R site-directed mutagenesis Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 additional information
-
additional information kinetic analysis, overview Rattus norvegicus
2.5.1.18 additional information
-
additional information kinetic analysis of wild-type and mutant enzymes, overview Mus musculus
2.5.1.18 additional information
-
additional information kinetic analysis of wild-type and mutant enzymes, overview Homo sapiens
2.5.1.18 0.05
-
iodohexane pH 8.0, 30°C, recombinant enzyme Rattus norvegicus
2.5.1.18 0.058
-
iodohexane pH 8.0, 30°C, recombinant wild-type enzyme Homo sapiens
2.5.1.18 0.059
-
1-iodohexane pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
2.5.1.18 0.083
-
iodobutane pH 8.0, 30°C, recombinant wild-type enzyme Homo sapiens
2.5.1.18 0.085
-
iodomethane pH 8.0, 30°C, recombinant wild-type enzyme Homo sapiens
2.5.1.18 0.36
-
iodobutane pH 8.0, 30°C, recombinant enzyme Rattus norvegicus
2.5.1.18 0.56
-
1-Iodobutane pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
2.5.1.18 0.91
-
1-iodomethane pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
2.5.1.18 1.89
-
iodomethane pH 8.0, 30°C, recombinant enzyme Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Homo sapiens P30711
-
-
2.5.1.18 Mus musculus
-
-
-
2.5.1.18 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain XL1-Blue by nickel affinity chromatography Mus musculus
2.5.1.18 recombinant His-tagged wild-type enzyme from Escherichia coli strain XL1-Blue by nickel affinity chromatography Rattus norvegicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 additional information
-
substrate specificities of recombinant His-tagged wild-type and mutant enzymes, overview Mus musculus
2.5.1.18 additional information
-
substrate specificities of recombinant His-tagged wild-type and mutant enzymes, overview Homo sapiens
2.5.1.18 additional information
-
substrate specificity of recombinant His-tagged enzyme, overview Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1,2-epoxy-3-(4-nitrophenoxy)-propane + glutathione
-
Mus musculus ?
-
?
2.5.1.18 1,2-epoxy-3-(4-nitrophenoxy)-propane + glutathione
-
Rattus norvegicus ?
-
?
2.5.1.18 1,2-epoxy-3-(4-nitrophenoxy)-propane + glutathione
-
Homo sapiens ?
-
?
2.5.1.18 4-nitrobenzyl chloride + glutathione
-
Mus musculus ?
-
?
2.5.1.18 4-nitrobenzyl chloride + glutathione
-
Rattus norvegicus ?
-
?
2.5.1.18 4-nitrobenzyl chloride + glutathione
-
Homo sapiens ?
-
?
2.5.1.18 4-nitrophenethyl bromide + glutathione
-
Mus musculus ?
-
?
2.5.1.18 4-nitrophenethyl bromide + glutathione
-
Rattus norvegicus ?
-
?
2.5.1.18 4-nitrophenethyl bromide + glutathione
-
Homo sapiens ?
-
?
2.5.1.18 allyl isothiocyanate + glutathione
-
Mus musculus ?
-
?
2.5.1.18 allyl isothiocyanate + glutathione
-
Rattus norvegicus ?
-
?
2.5.1.18 allyl isothiocyanate + glutathione
-
Homo sapiens ?
-
?
2.5.1.18 benzyl isothiocyanate + glutathione
-
Mus musculus ?
-
?
2.5.1.18 benzyl isothiocyanate + glutathione
-
Rattus norvegicus ?
-
?
2.5.1.18 benzyl isothiocyanate + glutathione
-
Homo sapiens ?
-
?
2.5.1.18 cumene hydroperoxide + glutathione
-
Mus musculus ?
-
?
2.5.1.18 cumene hydroperoxide + glutathione
-
Rattus norvegicus ?
-
?
2.5.1.18 cumene hydroperoxide + glutathione
-
Homo sapiens ?
-
?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
Mus musculus S-(2,4-dinitrophenyl)glutathione + HCl
-
?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
Rattus norvegicus S-(2,4-dinitrophenyl)glutathione + HCl
-
?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
Homo sapiens S-(2,4-dinitrophenyl)glutathione + HCl
-
?
2.5.1.18 glutathione + 1-iodobutane
-
Mus musculus iodide + butyl-glutathione
-
?
2.5.1.18 glutathione + 1-iodohexane
-
Mus musculus iodide + hexyl-glutathione
-
?
2.5.1.18 glutathione + 1-iodomethane
-
Mus musculus iodide + methylglutathione
-
?
2.5.1.18 hydrogen peroxide + glutathione
-
Mus musculus ?
-
?
2.5.1.18 hydrogen peroxide + glutathione
-
Rattus norvegicus ?
-
?
2.5.1.18 hydrogen peroxide + glutathione
-
Homo sapiens ?
-
?
2.5.1.18 iodobutane + glutathione
-
Rattus norvegicus ?
-
?
2.5.1.18 iodohexane + glutathione
-
Rattus norvegicus ?
-
?
2.5.1.18 iodomethane + glutathione
-
Rattus norvegicus ?
-
?
2.5.1.18 additional information substrate specificity, overview. A major structural determinant of substrate recognition is the H-site, which binds the electrophile in proximity to the nucleophilic sulfur of the second substrate glutathione. H-site residue 234 has a key role in governing the activity and substrate selectivity profile of GST T1-1 Mus musculus ?
-
?
2.5.1.18 additional information substrate specificity, overview. A major structural determinant of substrate recognition is the H-site, which binds the electrophile in proximity to the nucleophilic sulfur of the second substrate glutathione. H-site residue 234 has a key role in governing the activity and substrate selectivity profile of GST T1-1 Rattus norvegicus ?
-
?
2.5.1.18 additional information substrate specificity, overview. A major structural determinant of substrate recognition is the H-site, which binds the electrophile in proximity to the nucleophilic sulfur of the second substrate glutathione. H-site residue 234 has a key role in governing the activity and substrate selectivity profile of GST T1-1 Homo sapiens ?
-
?
2.5.1.18 phenethyl isothiocyanate + glutathione
-
Mus musculus ?
-
?
2.5.1.18 phenethyl isothiocyanate + glutathione
-
Rattus norvegicus ?
-
?
2.5.1.18 phenethyl isothiocyanate + glutathione
-
Homo sapiens ?
-
?
2.5.1.18 propyl isothiocyanate + glutathione
-
Mus musculus ?
-
?
2.5.1.18 propyl isothiocyanate + glutathione
-
Rattus norvegicus ?
-
?
2.5.1.18 propyl isothiocyanate + glutathione
-
Homo sapiens ?
-
?
2.5.1.18 styrene oxide + glutathione
-
Mus musculus ?
-
?
2.5.1.18 styrene oxide + glutathione
-
Rattus norvegicus ?
-
?
2.5.1.18 styrene oxide + glutathione
-
Homo sapiens ?
-
?
2.5.1.18 tertiary butyl hydroperoxide + glutathione
-
Mus musculus ?
-
?
2.5.1.18 tertiary butyl hydroperoxide + glutathione
-
Rattus norvegicus ?
-
?
2.5.1.18 tertiary butyl hydroperoxide + glutathione
-
Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 More the substrate recognition H-site is formed by several segments of amino acid residues located in separate regions of the primary structure. The C-terminal helix of the protein serves as a lid over the active site, and contributes several residues to the H-site, molecular modeling, overview Mus musculus
2.5.1.18 More the substrate recognition H-site is formed by several segments of amino acid residues located in separate regions of the primary structure. The C-terminal helix of the protein serves as a lid over the active site, and contributes several residues to the H-site, molecular modeling, overview Rattus norvegicus
2.5.1.18 More the substrate recognition H-site is formed by several segments of amino acid residues located in separate regions of the primary structure. The C-terminal helix of the protein serves as a lid over the active site, and contributes several residues to the H-site, molecular modeling, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 GST T1-1
-
Mus musculus
2.5.1.18 GST T1-1
-
Rattus norvegicus
2.5.1.18 GST T1-1
-
Homo sapiens
2.5.1.18 theta class glutathione transferase T1-1
-
Mus musculus
2.5.1.18 theta class glutathione transferase T1-1
-
Rattus norvegicus
2.5.1.18 theta class glutathione transferase T1-1
-
Homo sapiens
2.5.1.18 theta class GST
-
Mus musculus
2.5.1.18 theta class GST
-
Rattus norvegicus
2.5.1.18 theta class GST
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 30
-
assay at Mus musculus
2.5.1.18 30
-
assay at Rattus norvegicus
2.5.1.18 30
-
assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 0.037
-
iodobutane pH 8.0, 30°C, recombinant wild-type enzyme Homo sapiens
2.5.1.18 0.068
-
iodohexane pH 8.0, 30°C, recombinant wild-type enzyme Homo sapiens
2.5.1.18 0.137
-
1-Iodobutane pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
2.5.1.18 0.215
-
iodobutane pH 8.0, 30°C, recombinant enzyme Rattus norvegicus
2.5.1.18 3.17
-
1-iodohexane pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
2.5.1.18 3.52
-
iodohexane pH 8.0, 30°C, recombinant enzyme Rattus norvegicus
2.5.1.18 9.16
-
iodomethane pH 8.0, 30°C, recombinant wild-type enzyme Homo sapiens
2.5.1.18 238
-
1-iodomethane pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
2.5.1.18 349
-
iodomethane pH 8.0, 30°C, recombinant enzyme Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 6.5 8 assay at, dependent on the substrate Mus musculus
2.5.1.18 6.5 8 assay at, dependent on the substrate Rattus norvegicus
2.5.1.18 6.5 8 assay at, dependent on the substrate Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.18 0.244
-
iodobutane pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
2.5.1.18 0.59
-
iodobutane pH 8.0, 30°C, recombinant enzyme Rattus norvegicus
2.5.1.18 0.701
-
iodobutane pH 8.0, 30°C, recombinant wild-type enzyme Homo sapiens
2.5.1.18 1.17
-
iodohexane pH 8.0, 30°C, recombinant wild-type enzyme Homo sapiens
2.5.1.18 54.1
-
iodohexane pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
2.5.1.18 69.8
-
iodohexane pH 8.0, 30°C, recombinant enzyme Rattus norvegicus
2.5.1.18 107
-
iodomethane pH 8.0, 30°C, recombinant wild-type enzyme Homo sapiens
2.5.1.18 185
-
iodomethane pH 8.0, 30°C, recombinant enzyme Rattus norvegicus
2.5.1.18 263
-
iodomethane pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus