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Literature summary extracted from

  • Kaushik, V.K.; Kavana, M.; Volz, J.M.; Weldon, S.C.; Hanrahan, S.; Xu, J.; Caplan, S.L.; Hubbard, B.K.
    Characterization of recombinant human acetyl-CoA carboxylase-2 steady-state kinetics (2009), Biochim. Biophys. Acta, 1794, 961-967.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.4.1.2 citrate activates both by increasing kcat and kcat/KM for ATP and acetyl-CoA, has a putative regulatory function on ACC2 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.4.1.2 expression in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.2 ATP competitive inhibition versus ADP Homo sapiens
6.4.1.2 HCO3- competitive inhibition versus phosphate Homo sapiens
6.4.1.2 malonyl-CoA competitive inhibition versus acetyl-CoA Homo sapiens
6.4.1.2 additional information product inhibition analysis, inhibition patterns, overview Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.2 additional information
-
 additional information steady-state kinetic analysis of ACC2, kinetic mechanism, overview Homo sapiens
6.4.1.2 0.048
-
 acetyl-CoA pH 7.5, recombinant enzyme Homo sapiens
6.4.1.2 0.125
-
 ATP pH 7.5, recombinant enzyme Homo sapiens
6.4.1.2 2.1
-
 HCO3- pH 7.5, recombinant enzyme Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.2 Mg2+ dependent on Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3- Homo sapiens
-
 ADP + phosphate + malonyl-CoA malonyl-CoA is a key metabolite in the fatty acid synthetic and oxidation pathways ir
6.4.1.2 ATP + acetyl-CoA + HCO3- + H+ Homo sapiens
-
 ADP + phosphate + malonyl-CoA malonyl-CoA is a key metabolite in the fatty acid synthetic and oxidation pathways ir

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.2 recombinant enzyme from Sf9 insect cells by ammonium sulfate fractionation and anion exchange chromatography Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
6.4.1.2 ATP + acetyl-CoA + hydrogencarbonate = ADP + phosphate + malonyl-CoA the enzyme proceeds through a hybrid (two-site) random Ter Ter mechanism, one that likely involves a two-step reaction at the biotin carboxylase domain Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Homo sapiens ADP + phosphate + malonyl-CoA
-
 ir
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Homo sapiens ADP + phosphate + malonyl-CoA malonyl-CoA is a key metabolite in the fatty acid synthetic and oxidation pathways ir
6.4.1.2 ATP + acetyl-CoA + HCO3- + H+
-
 Homo sapiens ADP + phosphate + malonyl-CoA
-
 ir
6.4.1.2 ATP + acetyl-CoA + HCO3- + H+
-
 Homo sapiens ADP + phosphate + malonyl-CoA malonyl-CoA is a key metabolite in the fatty acid synthetic and oxidation pathways ir

Synonyms

EC Number Synonyms Comment Organism
6.4.1.2 ACC2
-
 Homo sapiens
6.4.1.2 acetyl-CoA carboxylase-2
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.4.1.2 2.9
-
 acetyl-CoA pH 7.5, recombinant enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.2 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.2 ATP
-
 Homo sapiens
6.4.1.2 biotin ACC2 is a biotinylated enzyme Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.4.1.2 additional information
-
 additional information product inhibition kinetics, detailed overview Homo sapiens