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Literature summary extracted from

  • Calero-Rueda, O.; Barba, V.; Rodriguez, E.; Plou, F.; Martinez, A.T.; Martinez, M.J.
    Study of a sterol esterase secreted by Ophiostoma piceae: sequence, model and biochemical properties (2009), Biochim. Biophys. Acta, 1794, 1099-1106.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.13 expressed in Pichia pastoris, resulting in higher activities than those obtained in the Ophistoma piceae cultures Ophiostoma piceae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.13 58200
-
 MALDI-TOF, SDS-PAGE Ophiostoma piceae

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.13 Ophiostoma piceae Q2TFW1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.13 The Ophiostoma piceae esterase is purified from 15-day-old cultures on glucose-peptone-yeast extract medium supplemented with 0.5% olive oil. Recombinant protein is purified following the same procedure from 7-day-old cultures growing on BMMY medium Ophiostoma piceae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.13 79.84
-
 substrate cholesteryl oleate, native enzyme in the presence of 5% Genapol X-100 Ophiostoma piceae
3.1.1.13 171
-
 substrate triolein, native enzyme in the presence of 5% Genapol X-100 Ophiostoma piceae
3.1.1.13 319
-
 substrate cholesteryl oleate, recombinant enzyme in the presence of 5% Genapol X-100 Ophiostoma piceae
3.1.1.13 449.7
-
 substrate 4-nitrophenyl butyrate, native enzyme in the presence of 1% Genapol X-100 Ophiostoma piceae
3.1.1.13 698.1
-
 substrate triolein, recombinant enzyme in the presence of 5% Genapol X-100 Ophiostoma piceae
3.1.1.13 1336
-
 substrate 4-nitrophenyl butyrate, recombinant enzyme in the presence of 1% Genapol X-100 Ophiostoma piceae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.13 cholesteryl oleate + H2O
-
 Ophiostoma piceae cholesterol + oleate
-
 ?
3.1.1.13 p-nitrophenyl butyrate + H2O
-
 Ophiostoma piceae p-nitrophenol + butanoic acid
-
 ?
3.1.1.13 p-nitrophenyl palmitate + H2O
-
 Ophiostoma piceae p-nitrophenol + palmitic acid
-
 ?
3.1.1.13 tributyrin + H2O
-
 Ophiostoma piceae dibutyrin + butyrate
-
 ?
3.1.1.13 triolein + H2O
-
 Ophiostoma piceae diolein + oleate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.13 OPE
-
 Ophiostoma piceae
3.1.1.13 Sterol esterase
-
 Ophiostoma piceae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.13 25
-
 assay at Ophiostoma piceae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.13 7
-
 assay at Ophiostoma piceae