EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.33 | additional information | positional specificity of wild-type 15-LOX-2 does hardly depend on the pH of the reaction buffer. The pH sensitivity can be induced by introduction of a His at the active site | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.31 | ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) | Mus musculus |
1.13.11.31 | ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) | Danio rerio |
1.13.11.33 | ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) | Mus musculus |
1.13.11.33 | ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) | Oryctolagus cuniculus |
1.13.11.33 | LOX isoforms ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) | Homo sapiens |
1.13.11.40 | ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.13.11.33 | D602Y | 15-LOX-2 mutant shows a similar pH-profile as the wild-type enzyme | Homo sapiens |
1.13.11.33 | D602Y/V603H | a strong shift of the pH profile of 15-LOX-2 mutant towards more acidic values | Homo sapiens |
1.13.11.33 | V603H | a strong shift of the pH profile of 15-LOX-2 mutant towards more acidic values. 15-H(p)ETE is the major oxygenation product at pH 8 | Homo sapiens |
1.13.11.40 | H604F | 8-LOX mutant, induces strong pH-dependent alterations in the positional specificity but the pH-optimum remains the same. At acidic pH 8S-H(p)ETE is the exclusive arachidonic acid oxygenation product but with more alkaline pH increasing shares of 15S-H(p)ETE, above pH 9 15S-H(p)ETE is the major oxygenation product. Specific activity of 8-LOX mutant with (5Z,8Z,11Z,14Z)-nonadeca-5,8,11,14-tetraene-1,19-dioic acid as substrate continously declines when pH increases | Mus musculus |
1.13.11.40 | Y603F | 8-LOX mutant, is relative insensitive towards pH alterations in the near physiological range (pH 6-8), at strong alkaline conditions (pH more than 9) significant shares of 15S-H(p)ETE are formed | Mus musculus |
1.13.11.40 | Y603F/H604F | 8-LOX double mutant, induces strong pH-dependent alterations in the positional specificity but the pH-optimum remains the same. At acidic pH 8S-H(p)ETE is the exclusive arachidonic acid oxygenation product but with more alkaline pH increasing shares of 15S-H(p)ETE, above pH 9 15S-H(p)ETE is the major oxygenation product | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.31 | additional information | physico-chemical state of the substrate and the complex equilibrium between fatty acid monomers, acid soaps and micelles may impact the reaction specificity of LOX-isoforms | Mus musculus | |
1.13.11.33 | additional information | physico-chemical state of the substrate and the complex equilibrium between fatty acid monomers, acid soaps and micelles may impact the reaction specificity of LOX-isoforms | Homo sapiens | |
1.13.11.33 | additional information | physico-chemical state of the substrate and the complex equilibrium between fatty acid monomers, acid soaps and micelles may impact the reaction specificity of LOX-isoforms | Mus musculus | |
1.13.11.33 | additional information | pH alterations in the near physiological range impact the iron content of LOX and thus the catalytic activity | Oryctolagus cuniculus | |
1.13.11.40 | additional information | physico-chemical state of the substrate and the complex equilibrium between fatty acid monomers, acid soaps and micelles may impact the reaction specificity of LOX-isoforms | Mus musculus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.33 | Fe | - |
Oryctolagus cuniculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.31 | Danio rerio | - |
- |
- |
1.13.11.31 | Mus musculus | - |
- |
- |
1.13.11.33 | Homo sapiens | - |
- |
- |
1.13.11.33 | Mus musculus | - |
- |
- |
1.13.11.33 | Oryctolagus cuniculus | - |
- |
- |
1.13.11.40 | Mus musculus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.31 | on Ni column | Mus musculus |
1.13.11.31 | on Ni column | Danio rerio |
1.13.11.33 | on Ni column | Mus musculus |
1.13.11.33 | on Ni column | Homo sapiens |
1.13.11.33 | on Ni column | Oryctolagus cuniculus |
1.13.11.40 | on Ni column | Mus musculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.13.11.31 | skin | - |
Mus musculus | - |
1.13.11.33 | skin | - |
Mus musculus | - |
1.13.11.40 | skin | - |
Mus musculus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.40 | additional information | - |
specific activity of 8-LOX with (5Z,8Z,11Z,14Z)-nonadeca-5,8,11,14-tetraene-1,19-dioic acid as substrate strongly increases going from pH 6 to 7.2 but then drops down at more alkaline pH | Mus musculus |
EC Number | Storage Stability | Organism |
---|---|---|
1.13.11.31 | -80°C, 10% glycerol | Mus musculus |
1.13.11.31 | -80°C, 10% glycerol | Danio rerio |
1.13.11.33 | -80°C, 10% glycerol | Mus musculus |
1.13.11.33 | -80°C, 10% glycerol | Homo sapiens |
1.13.11.33 | -80°C, 10% glycerol | Oryctolagus cuniculus |
1.13.11.40 | -80°C, 10% glycerol | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.31 | arachidonate + O2 | - |
Mus musculus | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate | 12-H(p)ETE is the major reaction product of 12/15-LOX independent of the pH | ? | |
1.13.11.31 | arachidonate + O2 | - |
Danio rerio | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate | 15-H(p)ETE is the major reaction product independent of the pH | ? | |
1.13.11.33 | arachidonate + O2 | - |
Mus musculus | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | - |
? | |
1.13.11.33 | arachidonate + O2 | - |
Oryctolagus cuniculus | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | 15-H(p)ETE is the major reaction product independent of the pH | ? | |
1.13.11.33 | arachidonate + O2 | - |
Homo sapiens | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | 15-H(p)ETE is the major reaction product of 15-LOX-2 and 12/15-LOX independent of the pH | ? | |
1.13.11.33 | additional information | 15-LOX-2 does not oxygenate (5Z,8Z,11Z,14Z)-nonadeca-5,8,11,14-tetraene-1,19-dioic acid at various pH | Homo sapiens | ? | - |
? | |
1.13.11.40 | (5Z,8Z,11Z,14Z)-nonadeca-5,8,11,14-tetraene-1,19-dioic acid + O2 | 8-LOX tolerates a carboxylic group in the substrate-binding pocket | Mus musculus | ? | - |
? | |
1.13.11.40 | arachidonate + O2 | - |
Mus musculus | (5Z,9E,11Z,14Z)-(8R)-8-hydroperoxyicosa-5,9,11,14-tetraenoate | 8-H(p)ETE is the major reaction product of 8-LOX independent of the pH | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.31 | 12-lipoxygenase | - |
Mus musculus |
1.13.11.31 | 12-LOX | - |
Danio rerio |
1.13.11.31 | 12/15-LOX | - |
Mus musculus |
1.13.11.33 | 12/15-LOX | - |
Mus musculus |
1.13.11.33 | 12/15-LOX | - |
Homo sapiens |
1.13.11.33 | 12/15-LOX | - |
Oryctolagus cuniculus |
1.13.11.33 | 15-lipoxygenase | - |
Mus musculus |
1.13.11.33 | 15-lipoxygenase-2 | - |
Homo sapiens |
1.13.11.33 | 15-LOX-1 | - |
Homo sapiens |
1.13.11.33 | 15-LOX-2 | - |
Homo sapiens |
1.13.11.40 | 8-lipoxygenase | - |
Mus musculus |
1.13.11.40 | 8-LOX | - |
Mus musculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.33 | 8 | 9 | pH-optimum of 15-LOX-2 for arachidonic acid oxygenation | Homo sapiens |
1.13.11.40 | 8 | - |
8-LOX | Mus musculus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.33 | 6 | 9.5 | pH 6 about 50% of maximal activity, pH 9.5 about 60% of maximal activity | Homo sapiens |