BRENDA - Enzyme Database show

A lipoxygenase with dual positional specificity is expressed in olives (Olea europaea L.) during ripening

Palmieri-Thiers, C.; Canaan, S.; Brunini, V.; Lorenzi, V.; Tomi, F.; Desseyn, J.L.; Garscha, U.; Oliw, E.H.; Berti, L.; Maury, J.; Biochim. Biophys. Acta 1791, 339-346 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.13.11.B6
expression in Escherichia coli
Olea europaea
1.13.11.12
vector pQE30-LOX expressed in Escherichia coli M15 cells
Olea europaea
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.B6
nordihydroguaiaretic acid
noncompetitive
Olea europaea
1.13.11.B6
propyl gallate
competitive
Olea europaea
1.13.11.12
nordihydroguaiaretic acid
is a noncompetitive inhibitor
Olea europaea
1.13.11.12
propyl gallate
is a competitive inhibitor
Olea europaea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.B6
0.175
-
linoleate
25°C
Olea europaea
1.13.11.B6
0.344
-
linolenate
25°C
Olea europaea
1.13.11.12
0.1749
-
linoleic acid
pH 6.0, 25°C
Olea europaea
1.13.11.12
0.3437
-
linolenic acid
pH 6.0, 25°C
Olea europaea
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.B6
cytosol
likely confined to the cytosol, because it contains neither consensus targeting nor retention signals for any organelles
Olea europaea
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.B6
98000
-
x * 98000, SDS-PAGE
Olea europaea
1.13.11.B6
98400
-
x * 98400, calculated from sequence
Olea europaea
1.13.11.12
98000
-
x * 98000, SDS-PAGE and immunoblot
Olea europaea
1.13.11.12
98400
-
x * 98400, calculated from sequence
Olea europaea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.B6
Olea europaea
B6D1W5
L., Leccino variety
-
1.13.11.12
Olea europaea
B6D1W5
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.13.11.B6
-
Olea europaea
1.13.11.12
by Ni-affinity chromatography and gel filtration, 26fold
Olea europaea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.B6
fruit
detected in all olive developmental and ripening stages from green small fruits to black or senescent fruits. Mainly expressed at late developmental stages
Olea europaea
-
1.13.11.12
fruit
is mainly expressed at late developmental stages, two Lox genes expressed in black olives. Increase of the LOX activity at the end of the green stage and the turning stage of the olives, and the maximum is reached at the black stage
Olea europaea
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.13.11.B6
23.4
-
-
Olea europaea
1.13.11.12
23.4
-
26fold purified enzyme, with linoleic acid as substrate
Olea europaea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.B6
linoleate + O2
the catalytic efficiency of the recombinant olive LOX was significantly higher for linoleic acid hydroperoxidation than for linolenic acid hydroperoxidation
702431
Olea europaea
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E,13R)-13-hydroperoxy-9,11-octadecadienoate
dual positional specificity: the enzyme forms (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and (9Z,11E,13R)-13-hydroperoxy-9,11-octadecadienoate in a 2:1 ratio, the products are predominantly in 9S and 13R configuration
-
-
?
1.13.11.B6
linolenate + O2
the catalytic efficiency of the recombinant olive LOX is significantly higher for linoleic acid hydroperoxidation than for linolenic acid hydroperoxidation
702431
Olea europaea
?
-
-
-
?
1.13.11.12
linoleic acid + O2
-
702431
Olea europaea
(9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoic acid
the recombinant enzyme shows a dual positional specificity, as it forms both 9- and 13-hydroperoxy octadecadienoic acid in a ratio 2:1
-
-
?
1.13.11.12
linolenic acid + O2
-
702431
Olea europaea
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.13.11.B6
?
x * 98000, SDS-PAGE; x * 98400, calculated from sequence
Olea europaea
1.13.11.12
?
x * 98000, SDS-PAGE and immunoblot; x * 98400, calculated from sequence
Olea europaea
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.13.11.B6
47.8
-
linolenate
25°C
Olea europaea
1.13.11.B6
48.6
-
linoleate
25°C
Olea europaea
1.13.11.12
47.78
-
linolenic acid
pH 6.0, 25°C
Olea europaea
1.13.11.12
48.16
-
linoleic acid
pH 6.0, 25°C
Olea europaea
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.B6
6
-
-
Olea europaea
1.13.11.12
6
-
-
Olea europaea
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.13.11.B6
0.00188
-
propyl gallate
25°C
Olea europaea
1.13.11.B6
0.00409
-
nordihydroguaiaretic acid
25°C
Olea europaea
1.13.11.12
0.00188
-
propyl gallate
pH 6.0, 25°C
Olea europaea
1.13.11.12
0.00409
-
nordihydroguaiaretic acid
pH 6.0, 25°C
Olea europaea
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.13.11.B6
Olea europaea
calculated from sequence
-
5.95
1.13.11.12
Olea europaea
calculated from sequence
-
5.95
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.12
vector pQE30-LOX expressed in Escherichia coli M15 cells
Olea europaea
1.13.11.B6
expression in Escherichia coli
Olea europaea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.B6
nordihydroguaiaretic acid
noncompetitive
Olea europaea
1.13.11.B6
propyl gallate
competitive
Olea europaea
1.13.11.12
nordihydroguaiaretic acid
is a noncompetitive inhibitor
Olea europaea
1.13.11.12
propyl gallate
is a competitive inhibitor
Olea europaea
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.13.11.B6
0.00188
-
propyl gallate
25°C
Olea europaea
1.13.11.B6
0.00409
-
nordihydroguaiaretic acid
25°C
Olea europaea
1.13.11.12
0.00188
-
propyl gallate
pH 6.0, 25°C
Olea europaea
1.13.11.12
0.00409
-
nordihydroguaiaretic acid
pH 6.0, 25°C
Olea europaea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.B6
0.175
-
linoleate
25°C
Olea europaea
1.13.11.B6
0.344
-
linolenate
25°C
Olea europaea
1.13.11.12
0.1749
-
linoleic acid
pH 6.0, 25°C
Olea europaea
1.13.11.12
0.3437
-
linolenic acid
pH 6.0, 25°C
Olea europaea
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.B6
cytosol
likely confined to the cytosol, because it contains neither consensus targeting nor retention signals for any organelles
Olea europaea
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.B6
98000
-
x * 98000, SDS-PAGE
Olea europaea
1.13.11.B6
98400
-
x * 98400, calculated from sequence
Olea europaea
1.13.11.12
98000
-
x * 98000, SDS-PAGE and immunoblot
Olea europaea
1.13.11.12
98400
-
x * 98400, calculated from sequence
Olea europaea
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.B6
-
Olea europaea
1.13.11.12
by Ni-affinity chromatography and gel filtration, 26fold
Olea europaea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.B6
fruit
detected in all olive developmental and ripening stages from green small fruits to black or senescent fruits. Mainly expressed at late developmental stages
Olea europaea
-
1.13.11.12
fruit
is mainly expressed at late developmental stages, two Lox genes expressed in black olives. Increase of the LOX activity at the end of the green stage and the turning stage of the olives, and the maximum is reached at the black stage
Olea europaea
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.13.11.B6
23.4
-
-
Olea europaea
1.13.11.12
23.4
-
26fold purified enzyme, with linoleic acid as substrate
Olea europaea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.B6
linoleate + O2
the catalytic efficiency of the recombinant olive LOX was significantly higher for linoleic acid hydroperoxidation than for linolenic acid hydroperoxidation
702431
Olea europaea
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E,13R)-13-hydroperoxy-9,11-octadecadienoate
dual positional specificity: the enzyme forms (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and (9Z,11E,13R)-13-hydroperoxy-9,11-octadecadienoate in a 2:1 ratio, the products are predominantly in 9S and 13R configuration
-
-
?
1.13.11.B6
linolenate + O2
the catalytic efficiency of the recombinant olive LOX is significantly higher for linoleic acid hydroperoxidation than for linolenic acid hydroperoxidation
702431
Olea europaea
?
-
-
-
?
1.13.11.12
linoleic acid + O2
-
702431
Olea europaea
(9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoic acid
the recombinant enzyme shows a dual positional specificity, as it forms both 9- and 13-hydroperoxy octadecadienoic acid in a ratio 2:1
-
-
?
1.13.11.12
linolenic acid + O2
-
702431
Olea europaea
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.13.11.B6
?
x * 98000, SDS-PAGE; x * 98400, calculated from sequence
Olea europaea
1.13.11.12
?
x * 98000, SDS-PAGE and immunoblot; x * 98400, calculated from sequence
Olea europaea
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.13.11.B6
47.8
-
linolenate
25°C
Olea europaea
1.13.11.B6
48.6
-
linoleate
25°C
Olea europaea
1.13.11.12
47.78
-
linolenic acid
pH 6.0, 25°C
Olea europaea
1.13.11.12
48.16
-
linoleic acid
pH 6.0, 25°C
Olea europaea
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.B6
6
-
-
Olea europaea
1.13.11.12
6
-
-
Olea europaea
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.13.11.B6
Olea europaea
calculated from sequence
-
5.95
1.13.11.12
Olea europaea
calculated from sequence
-
5.95
General Information
EC Number
General Information
Commentary
Organism
1.13.11.B6
physiological function
the 9/13-LOX is associated with the ripening and senescence processes. Due to its dual positional specificity and its expression pattern, its contribution to the elaboration of the olive oil aroma might be considered
Olea europaea
1.13.11.12
physiological function
9/13-LOX is associated with the ripening and senescence processes
Olea europaea
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.13.11.B6
physiological function
the 9/13-LOX is associated with the ripening and senescence processes. Due to its dual positional specificity and its expression pattern, its contribution to the elaboration of the olive oil aroma might be considered
Olea europaea
1.13.11.12
physiological function
9/13-LOX is associated with the ripening and senescence processes
Olea europaea
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.13.11.B6
139
-
linolenate
25°C
Olea europaea
1.13.11.B6
278
-
linoleate
25°C
Olea europaea
1.13.11.12
140
-
linolenic acid
-
Olea europaea
1.13.11.12
280
-
linoleic acid
-
Olea europaea
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.13.11.B6
139
-
linolenate
25°C
Olea europaea
1.13.11.B6
278
-
linoleate
25°C
Olea europaea
1.13.11.12
140
-
linolenic acid
-
Olea europaea
1.13.11.12
280
-
linoleic acid
-
Olea europaea