Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nguyen, S.D.; Hung, N.D.; Cheon-Ho, P.; Ree, K.M.; Dai-Eun, S.
    Oxidative inactivation of lactonase activity of purified human paraoxonase 1 (PON1) (2009), Biochim. Biophys. Acta, 1790, 155-160.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.2 ascorbate 0.5 mM inhibits by ca. 19%. Ascorbate/Cu2+ (0.5 mM/0.001 mM) system shows ca. 91% inactivation Homo sapiens
3.1.1.2 Cu2+ 0.001 mM inhibits by ca. 13%. Ascorbate/Cu2+ (0.5 mM/0.001 mM) system shows ca. 91% inactivation. Ascorbate/Fe2+ (0.5 mM/0.002 mM) system shows ca. 51% inactivation after 30 min Homo sapiens
3.1.1.2 Fe2+ ascorbate/Fe2+ (0.5 mM/0.002 mM) system shows ca. 51% inactivation after 30 min Homo sapiens
3.1.1.2 additional information 1 mM 3-morpholinosydnoimine, 0.002 mM Fe2+ or 0.01 mM Mn2+, Co2+, Zn2+ cause no significant loss of activity Homo sapiens
3.1.1.2 sodium hypochlorite at 1 mM causes approximately 15% decrease in activity, at 1 mM and in the presence of PBS buffer causes approximately 76% decrease in activity Homo sapiens
3.1.1.17 ascorbate/Cu2+ oxidative system ascorbate/Cu2+ is the most potent in inactivating the lactonase activity of purified PON1, 0.5 mM/0.001 mM: up to 100% inhibition, 0.1 mM/0.001 mM: 90% inhibition, 95%-100% inhibition. Oleic acid prevents the ascorbate/Cu2+-induced inactivation Homo sapiens
3.1.1.17 ascorbate/Fe2+ 0.5 mM/0.002 mM: 20% inhibiiton Homo sapiens
3.1.1.17 Cu2+ 0.001 mM, 30% inhibition Homo sapiens
3.1.1.17 NaOCl 1 mM, 20% inhibition Homo sapiens
3.1.1.25 ascorbate ascorbate/Cu2+ system is the most potent in inactivating the lactonase activity (by 90%). Cu2+ remarkably enhances the inactivation in the presence of ascorbate. Ascorbate/Cu2+ (0.5 mM/0.001 mM) system shows ca. 95% inactivation. Ascorbate/Cu2+-mediated inactivation of lactonase activity is prevented by catalase (90%), oleic acid (73% at 0.01 mM) and dioleoylphosphatidylcholine (68% at 0.1 mM). Ascorbate/Fe2+ (0.5 mM/0.002 mM) system shows 20% inactivation Homo sapiens
3.1.1.25 Cu2+ inhibits in a concentration-dependent manner. Cu2+ alone inhibits the lactonase activity by 30% at concentrations as low as 0.001 mM. Ascorbate/Cu2+ system is the most potent in inactivating the lactonase activity (by 90%). Cu2+ remarkably enhances the inactivation in the presence of ascorbate. Ascorbate/Cu2+ (0.5 mM/0.001 mM) system shows ca. 95% inactivation. Ascorbate/Cu2+-mediated inactivation of lactonase activity is prevented by catalase (90%), oleic acid (73% at 0.01 mM) and dioleoylphosphatidylcholine (68% at 0.1 mM) Homo sapiens
3.1.1.25 Fe2+ ascorbate/Fe2+ (0.5 mM/0.002 mM) system shows 20% inactivation. Fe2+ alone shows no inhibition Homo sapiens
3.1.1.25 additional information neither ascorbate alone nor Mn2+, Zn2+, Co2+ or 3-morpholinosydnoimine cause a significant loss of lactonase activity Homo sapiens
3.1.1.25 sodium hypochlorite at 1 mM and 10 mM causes approximately 18 and 34% decrease in lactonase activity, respectively Homo sapiens
3.1.8.1 ascorbate 0.5 mM inhibits by ca. 22%. Ascorbate/Cu2+ (0.5 mM/0.001 mM) system shows ca. 63% inactivation Homo sapiens
3.1.8.1 Cu2+ Cu2+ alone has no effect. Ascorbate/Cu2+ (0.5 mM/0.001 mM) system shows ca. 63% inactivation Homo sapiens
3.1.8.1 Fe2+ ascorbate/Fe2+ (0.5 mM/0.002 mM) system shows ca. 27% inactivation after 30 min Homo sapiens
3.1.8.1 additional information 1 mM 3-morpholinosydnoimine, 0.002 mM Fe2+ or 0.01 mM Mn2+, Co2+, Zn2+, or 0.001 mM Cu2+ cause no significant loss of activity Homo sapiens
3.1.8.1 sodium hypochlorite at 1 mM and in the presence of PBS buffer causes approximately 49% decrease in activity Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.2 Ca2+ required for the catalysis Homo sapiens
3.1.1.25 Ca2+ required for the catalysis. Shows a slight protection of 10% at 5 mM on ascorbate/Cu2+-induced inactivation of lactonase activity Homo sapiens
3.1.8.1 Ca2+ required for the catalysis Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.2 45000
-
 x * 45000, SDS-PAGE Homo sapiens
3.1.1.17 45000
-
 SDS-PAGE Homo sapiens
3.1.1.25 45000
-
 x * 45000, SDS-PAGE Homo sapiens
3.1.8.1 45000
-
 x * 45000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.2 Homo sapiens
-
-
-
3.1.1.17 Homo sapiens
-
-
-
3.1.1.25 Homo sapiens
-
-
-
3.1.8.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.2 by different types of chromatographies Homo sapiens
3.1.1.17 using different types of chromatographies Homo sapiens
3.1.1.25 by different types of chromatographies Homo sapiens
3.1.8.1 by different types of chromatographies Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.2 plasma
-
 Homo sapiens
-
3.1.1.17 plasma
-
 Homo sapiens
-
3.1.1.25 plasma
-
 Homo sapiens
-
3.1.8.1 plasma
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.2 721
-
-
 Homo sapiens
3.1.8.1 0.391
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.2 phenyl acetate + H2O
-
 Homo sapiens phenol + acetate
-
 ?
3.1.1.17 valerolactone + H2O
-
 Homo sapiens ?
-
 ?
3.1.1.25 delta-valerolactone + H2O
-
 Homo sapiens 5-hydroxypentanoic acid
-
 ?
3.1.8.1 paraoxon + H2O
-
 Homo sapiens 4-nitrophenol + diethyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.2 ? x * 45000, SDS-PAGE Homo sapiens
3.1.1.25 ? x * 45000, SDS-PAGE Homo sapiens
3.1.8.1 ? x * 45000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.2 arylesterase
-
 Homo sapiens
3.1.1.17 human paraoxonase 1
-
 Homo sapiens
3.1.1.17 PON1
-
 Homo sapiens
3.1.1.25 lactonase
-
 Homo sapiens
3.1.8.1 paraoxonase 1
-
 Homo sapiens
3.1.8.1 PON1
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.17 8.3
-
 assay at Homo sapiens