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Literature summary extracted from

  • Pettersson, H.; Norlin, M.; Andersson, U.; Pikuleva, I.; Bjoerkhem, I.; Misharin, A.; Wikvall, K.
    Metabolism of a novel side chain modified DELTA8(14)-15-ketosterol, a potential cholesterol lowering drug: 28-hydroxylation by CYP27A1 (2008), Biochim. Biophys. Acta, 1781, 383-390.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.15 0.0047
-
 3beta-hydroxy-5alpha-cholest-8(14)-en-15-one pH 7.4, 37°C Homo sapiens
1.14.15.15 0.017
-
 (24S)-3beta-hydroxy-24-methyl-5alpha-cholesta-8(14),22-dien-15-one pH 7.4, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.15 microsome
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.15 (24S)-3beta-hydroxy-24-methyl-5alpha-cholesta-8(14),22-dien-15-one + reduced adrenodoxin + H+ + O2 Homo sapiens the substrate is a potential drug for lowering cholesterol in the treatment and/or prevention of coronary artery disease, but it is rapidly metabolized in the liver ?
-
 ?
1.14.15.15 3beta-hydroxy-5alpha-cholest-8(14)-en-15-one + reduced adrenodoxin + H+ + O2 Homo sapiens the substrate is a potential drug for lowering cholesterol in the treatment and/or prevention of coronary artery disease, but it is rapidly metabolized in the liver ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.15 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.15 HEK-293 cell
-
 Homo sapiens
-
1.14.15.15 Hep-G2 cell
-
 Homo sapiens
-
1.14.15.15 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.15 (24S)-3beta-hydroxy-24-methyl-5alpha-cholesta-8(14),22-dien-15-one + reduced adrenodoxin + H+ + O2 the substrate is a potential drug for lowering cholesterol in the treatment and/or prevention of coronary artery disease, but it is rapidly metabolized in the liver Homo sapiens ?
-
 ?
1.14.15.15 (24S)-3beta-hydroxy-24-methyl-5alpha-cholesta-8(14),22-dien-15-one + reduced adrenodoxin + H+ + O2 28-hydroxylation by CYP27A1, enzyme activity assay in presence of adrenodoxin, adrenodoxin reductase and NADPH Homo sapiens ? massspectrometric product analysis, 2 products, overview ?
1.14.15.15 3beta-hydroxy-5alpha-cholest-8(14)-en-15-one + reduced adrenodoxin + H+ + O2 the substrate is a potential drug for lowering cholesterol in the treatment and/or prevention of coronary artery disease, but it is rapidly metabolized in the liver Homo sapiens ?
-
 ?
1.14.15.15 3beta-hydroxy-5alpha-cholest-8(14)-en-15-one + reduced adrenodoxin + H+ + O2 28-hydroxylation by CYP27A1, enzyme activity assay in presence of adrenodoxin, adrenodoxin reductase and NADPH Homo sapiens ? massspectrometric product analysis, 2 products, overview ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.15 CYP27A1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.15 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.15.15 0.0183
-
 (24S)-3beta-hydroxy-24-methyl-5alpha-cholesta-8(14),22-dien-15-one pH 7.4, 37°C, kcat for the conversion of (24S)-3beta-hydroxy-methyl-5alpha-cholesta-8(14),22-dien-15-one into side chain oxygenated products Homo sapiens
1.14.15.15 0.073
-
 3beta-hydroxy-5alpha-cholest-8(14)-en-15-one pH 7.4, 37°C, kcat for the conversion of (24S)-3beta-hydroxy-methyl-5alpha-cholesta-8(14),22-dien-15-one into side chain oxygenated products Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.15 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.15 NADPH
-
 Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.15.15 0.001
-
 3beta-hydroxy-5alpha-cholest-8(14)-en-15-one pH 7.4, 37°C Homo sapiens
1.14.15.15 0.0157
-
 (24S)-3beta-hydroxy-24-methyl-5alpha-cholesta-8(14),22-dien-15-one pH 7.4, 37°C Homo sapiens