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Literature summary extracted from

  • Blikstad, C.; Shokeer, A.; Kurtovic, S.; Mannervik, B.
    Emergence of a novel highly specific and catalytically efficient enzyme from a naturally promiscuous glutathione transferase (2008), Biochim. Biophys. Acta, 1780, 1458-1463.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 isozymes GST A1-1 and GST A4-4, expression in Escherichia coli strain XL-1 Blue, expression of mutant GIMFhelix in Escherichia coli strain Rosetta-gami B(DE3) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.18 additional information construction of mutant GIMFhelix by exchange of active site residues leading to about 300fold enhanced enzyme activity, substrate-activity profile compared to wild-type isozymes, overview. GIMFhelix contains all of the H-site residues identified in isozyme GST A4-4. GIMFhelix showed two orders of magnitude higher catalytic efficiency with long-chain alkenals than isozyme GST A1-1, while the activity with the standard substrate CDNB is one order below that of GST A1-1 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Homo sapiens
-
 isozymes GST A1-1 and GST A4-4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 recombinant isozymes GST A1-1 and GST A4-4 and recombinant mutant GIMFhelix from Escherichia coli by glutathione affinity chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1-methyl-4-nitro-5-[(4-nitrophenyl)thio]-1H-imidazole + glutathione
-
 Homo sapiens ?
-
 ?
2.5.1.18 4-nitrophenylacetate + glutathione
-
 Homo sapiens ?
-
 ?
2.5.1.18 androstenedione + glutathione
-
 Homo sapiens ?
-
 ?
2.5.1.18 butyl nitrite + glutathione
-
 Homo sapiens ?
-
 ?
2.5.1.18 cumene hydroperoxide + glutathione
-
 Homo sapiens ?
-
 ?
2.5.1.18 ethacrynic acid + glutathione
-
 Homo sapiens ?
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Homo sapiens S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 nonenal + glutathione
-
 Homo sapiens ?
-
 ?
2.5.1.18 phenethyl isothiocyanate + glutathione
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 GST
-
 Homo sapiens
2.5.1.18 GST A1-1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 7.4
-
 assay at Homo sapiens