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Literature summary extracted from

  • Chen, X.; Liu, J.; Yang, P.; Chen, D.
    Identifying functional residues in Arabidopsis thaliana zeta class glutathione S-transferase through screening inactive point mutants (2010), Biochemistry, 75, 110-114.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 GSTZ, library screening, cloning in Escherichia coli strain DH5alpha, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.18 C19Y random mutagenesis, inactive mutant Arabidopsis thaliana
2.5.1.18 K30E site-directed mutagenesis, inactive mutant Arabidopsis thaliana
2.5.1.18 K83E random mutagenesis, the mutant shows 50% reduced activity compared to the wild-type enzyme Arabidopsis thaliana
2.5.1.18 L11P site-directed mutagenesis, inactive mutant Arabidopsis thaliana
2.5.1.18 L11P/I140T site-directed mutagenesis, inactive mutant Arabidopsis thaliana
2.5.1.18 L147F random mutagenesis, the mutant shows 47.7% reduced activity compared to the wild-type enzyme Arabidopsis thaliana
2.5.1.18 L147P site-directed mutagenesis, inactive mutant Arabidopsis thaliana
2.5.1.18 L150P site-directed mutagenesis, inactive mutant Arabidopsis thaliana
2.5.1.18 L169R site-directed mutagenesis, inactive mutant Arabidopsis thaliana
2.5.1.18 L27P site-directed mutagenesis, inactive mutant Arabidopsis thaliana
2.5.1.18 L80P/N116S site-directed mutagenesis, inactive mutant Arabidopsis thaliana
2.5.1.18 additional information mutant enzyme inactivation mechanism, molecular modeling, mutations resulting in inactivation of the enzyme are found in or near the binding pocket, whereas mutations resulting in partial inactivation are distant from both substrates, overview Arabidopsis thaliana
2.5.1.18 P61S random mutagenesis, inactive mutant Arabidopsis thaliana
2.5.1.18 Q102R random mutagenesis, the mutant shows 68.4% reduced activity compared to the wild-type enzyme Arabidopsis thaliana
2.5.1.18 Q112R random mutagenesis, inactive mutant Arabidopsis thaliana
2.5.1.18 R22H/K83E random mutagenesis, inactive mutant Arabidopsis thaliana
2.5.1.18 S105R/Q213R site-directed mutagenesis, inactive mutant Arabidopsis thaliana
2.5.1.18 S109P random mutagenesis, inactive mutant Arabidopsis thaliana
2.5.1.18 S73P random mutagenesis, inactive mutant Arabidopsis thaliana
2.5.1.18 S73X site-directed mutagenesis, inactive mutant Arabidopsis thaliana
2.5.1.18 W15R random mutagenesis, inactive mutant Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 dichloroacetic acid + glutathione
-
 Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 More molecular modeling of the GSTZ substrate superimposed enzyme using the apoform crystal structure of AtGSTZ monomer Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 GSTZ
-
 Arabidopsis thaliana
2.5.1.18 zeta class glutathione S-transferase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 37
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 6.8
-
 assay at Arabidopsis thaliana