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Literature summary extracted from
- DNA repair by photolyase: a novel substrate with low background absorption around 265 nm for transient absorption studies in the UV (2010), Biochemistry, 49, 297-303.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 4.1.99.3 | analysis | the novel substrate (a modified thymidine 10-mer with a central CPD and all bases, except the one at the 3' end, replaced by 5,6-dihydrothymine) is a promising tool for fast and ultrafast transient absorption studies on pyrimidine dimer splitting by CPD photolyase | Escherichia coli |
| 4.1.99.3 | analysis | the novel substrate (a modified thymidine 10-mer with a central cyclobutane pyrimidine dimer and all bases, except the one at the 3' end, replaced by 5,6-dihydrothymine) is a promising tool for fast and ultrafast transient absorption studies on pyrimidine dimer splitting by CPD photolyase | Synechococcus elongatus PCC 7942 = FACHB-805 |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.99.3 | apophotolyase (devoid of the antenna cofactor 5,10-methenyltetrahydrofolate) overproduced in Escherichia coli | Synechococcus elongatus PCC 7942 = FACHB-805 |
| 4.1.99.3 | holophotolyase (both flavin and antenna cofactor 5,10-methenyltetrahydrofolate present) overexpressed | Escherichia coli |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.1.99.3 | E109A | lacks the antenna cofactor | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.99.3 | Escherichia coli | - |
- |
- |
| 4.1.99.3 | Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.99.3 | apophotolyase | Synechococcus elongatus PCC 7942 = FACHB-805 |
| 4.1.99.3 | holophotolyase | Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.1.99.3 | -80°C, 2-mercaptoethanol, 20% glycerol | Synechococcus elongatus PCC 7942 = FACHB-805 |
| 4.1.99.3 | -80°C, 2-mercaptoethanol, 20% glycerol | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.99.3 | additional information | a novel substrate (a modified thymidine 10-mer with a central cyclobutane pyrimidine dimer and all bases, except the one at the 3' end, replaced by 5,6-dihydrothymine) is repaired with an efficiency very similar to that of the conventional substrates (a 10-mer of unmodified thymidines containing a central cyclobutane pyrimidine dimer and an acetone-sensitized thymidine 18-mer containing in average six randomly distributed cyclobutane pyrimidine dimers per strand) | Synechococcus elongatus PCC 7942 = FACHB-805 | ? | - |
? |  |
| 4.1.99.3 | additional information | a novel substrate (a modified thymidine 10-mer with a central cyclobutane pyrimidine dimer and all bases, except the one at the 3' end, replaced by 5,6-dihydrothymine) is repaired with an efficiency very similar to that of the conventional substrates (a 10-mer of unmodified thymidines containing a central cyclobutane pyrimidine dimer and an acetone-sensitized thymidine 18-mer containing in average six randomly distributed cyclobutane pyrimidine dimers per strand). Significantly lower repair quantum yield for the holoenzyme compared to its apo form due to an additional process, i.e., excitation energy transfer from the antenna cofactor to the reduced flavin | Escherichia coli | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.99.3 | CPD photolyase | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
| 4.1.99.3 | CPD photolyase photolyase | - |
Escherichia coli |
| 4.1.99.3 | cyclobutane pyrimidine dimer photolyase | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
| 4.1.99.3 | cyclobutane pyrimidine dimer photolyase | - |
Escherichia coli |
| 4.1.99.3 | photolyase | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.99.3 | 5,10-methenyltetrahydrofolate | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 4.1.99.3 | 5,10-methenyltetrahydrofolate | antenna cofactor | Escherichia coli | |
| 4.1.99.3 | FAD | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 4.1.99.3 | FAD | - |
Escherichia coli | |
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