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Literature summary extracted from

  • Aaron, J.A.; Lin, X.; Cane, D.E.; Christianson, D.W.
    Structure of epi-isozizaene synthase from Streptomyces coelicolor A3(2), a platform for new terpenoid cyclization templates (2010), Biochemistry, 49, 1787-1797.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.37 His-tagged version expressed in Escherichia coli BL21(DE3) Streptomyces coelicolor

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.3.37 sitting drop vapor diffusion method Streptomyces coelicolor

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.37 D99N coordinates to Mg2+ Streptomyces coelicolor
4.2.3.37 F198A major products: Z-gamma-bisabolene (24%), sesqusabine (20%) Streptomyces coelicolor
4.2.3.37 F96A major product: beta-farnesene (70%) Streptomyces coelicolor
4.2.3.37 W203F major product: Z-gamma-bisabolene (47%) Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.37 0.00071
-
 (2E,6E)-farnesyl diphosphate wild-type protein Streptomyces coelicolor
4.2.3.37 0.00077
-
 (2E,6E)-farnesyl diphosphate F96A mutant protein Streptomyces coelicolor
4.2.3.37 0.0012
-
 (2E,6E)-farnesyl diphosphate F198A mutant protein Streptomyces coelicolor
4.2.3.37 0.00145
-
 (2E,6E)-farnesyl diphosphate F203F mutant protein Streptomyces coelicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.37 Mg2+
-
 Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.37 Streptomyces coelicolor Q9K499
-
-
4.2.3.37 Streptomyces coelicolor A3(2) Q9K499
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.37 immobilized metal ion affinity chromatography (Co2+) Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.37 (2E,6E)-farnesyl diphosphate
-
 Streptomyces coelicolor (+)-epi-isozizaene + diphosphate major product (79%) ?
4.2.3.37 (2E,6E)-farnesyl diphosphate
-
 Streptomyces coelicolor A3(2) (+)-epi-isozizaene + diphosphate major product (79%) ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.37 epi-isozizaene synthase
-
 Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.37 0.00024
-
 (2E,6E)-farnesyl diphosphate F96A mutant protein Streptomyces coelicolor
4.2.3.37 0.0003
-
 (2E,6E)-farnesyl diphosphate F198A mutant protein Streptomyces coelicolor
4.2.3.37 0.00034
-
 (2E,6E)-farnesyl diphosphate F203F mutant protein Streptomyces coelicolor
4.2.3.37 0.045
-
 (2E,6E)-farnesyl diphosphate wild-type protein Streptomyces coelicolor

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.37 0.23
-
 (2E,6E)-farnesyl diphosphate F203F mutant protein Streptomyces coelicolor
4.2.3.37 0.25
-
 (2E,6E)-farnesyl diphosphate F198A mutant protein Streptomyces coelicolor
4.2.3.37 0.31
-
 (2E,6E)-farnesyl diphosphate F96A mutant protein Streptomyces coelicolor
4.2.3.37 63
-
 (2E,6E)-farnesyl diphosphate wild-type protein Streptomyces coelicolor