EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.3.22 | cloned and expressed in Drosophila S2 cells | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.4.3.22 | the structure of the native enzyme is determined by X-ray crystallography to a resolution of 1.8 A. The homodimeric structure has the archetypal amine oxidase fold. Two active sites, one in each subunit, are characterized by the presence of a copper ion and a topaquinone residue formed. Substrate binding pocket and entry channel of hDAO are distinctly different from other amine oxidases in accord with the different substrate specificities. The structures of two inhibitor complexes of hDAO, berenil and pentamidine, are refined to resolutions of 2.1 and 2.2 A, respectively. They bind noncovalently in the active-site channel. The inhibitor binding suggests that an aspartic acid residue, conserved in all diamine oxidases but absent from other amine oxidases, is responsible for the diamine specificity by interacting with the second amino group of preferred diamine substrates | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.22 | 4,4'-(pentane-1,5-diylbis(oxy))dibenzimidamide | noncompetitive inhibition | Homo sapiens | |
1.4.3.22 | Berenil | mixed inhibition | Homo sapiens | |
1.4.3.22 | cimetidine | mixed inhibition | Homo sapiens | |
1.4.3.22 | clonidine | mixed inhibition | Homo sapiens | |
1.4.3.22 | isoniazid | noncompetitive inhibition | Homo sapiens | |
1.4.3.22 | Pentamidine | mixed inhibition | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.22 | Ca2+ | enzmye contains 2 Ca2+-binding sites per subunit | Homo sapiens | |
1.4.3.22 | copper | enzyme binds Cu | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.22 | Homo sapiens | P19801 | in human three functioning genes exist that encode copper-containing amine oxidases | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.4.3.22 | glycoprotein | putative N-glycosylation sites: Asn110, Asn538, and Asn745 | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.22 | putrescine + H2O + O2 | - |
Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.3.22 | homodimer | crystal structure | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.3.22 | Diamine oxidase | - |
Homo sapiens |
1.4.3.22 | hDAO | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.3.22 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.3.22 | 7.2 | - |
assay at | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.22 | topaquinone | - |
Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.22 | 0.0011 | - |
4,4'-(pentane-1,5-diylbis(oxy))dibenzimidamide | - |
Homo sapiens | |
1.4.3.22 | 0.013 | - |
Berenil | - |
Homo sapiens | |
1.4.3.22 | 0.09 | - |
cimetidine | - |
Homo sapiens | |
1.4.3.22 | 0.1 | - |
clonidine | - |
Homo sapiens | |
1.4.3.22 | 0.29 | - |
Pentamidine | - |
Homo sapiens | |
1.4.3.22 | 0.9 | - |
isoniazid | - |
Homo sapiens |