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Literature summary extracted from

  • Wecksler, A.T.; Kenyon, V.; Garcia, N.K.; Deschamps, J.D.; van der Donk, W.A.; Holman, T.R.
    Kinetic and structural investigations of the allosteric site in human epithelial 15-lipoxygenase-2 (2009), Biochemistry, 48, 8721-8730.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.33 13-(S)-hydroxyoctadecadienoic acid allosteric effector, which changes the conformation of 15-hLO-2 such that substrate affinity toward linoleic acid is significantly increased Homo sapiens

Application

EC Number Application Comment Organism
1.13.11.33 medicine pro-tumorigenic feedback loop for 13-(S)-hydroxyoctadecadienoic acid against 15-hLO-2 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.33 without a His6-tag Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.33 linoleic acid fully deuterated, the mode of inhibition, in the presence of 13-(S)-hydroxyoctadecadienoic acid, changes from mixed-type inhibition to competitive inhibition Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.33 Fe iron-containing metalloenzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.33 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.33 without a His6-tag, more than 90% pure Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.33 epithelium
-
 Homo sapiens
-
1.13.11.33 prostate
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.33 arachidonate + O2
-
 Homo sapiens (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.33 15-hLO-2
-
 Homo sapiens
1.13.11.33 15-lipoxygenase-2
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.33 0.57
-
 arachidonic acid at 15°C, normalized to iron content Homo sapiens
1.13.11.33 0.62
-
 arachidonic acid at pH 7 Homo sapiens
1.13.11.33 0.74
-
 arachidonic acid at pH 8 Homo sapiens
1.13.11.33 0.75
-
 arachidonic acid at pH 7.5 Homo sapiens
1.13.11.33 0.75
-
 arachidonic acid at 22°C, normalized to iron content Homo sapiens
1.13.11.33 1.04
-
 arachidonic acid at 30°C, normalized to iron content Homo sapiens
1.13.11.33 1.38
-
 arachidonic acid at 37°C, normalized to iron content Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.33 7 8 approximately level between pH 7 and 8 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.33 0.005
-
 linoleic acid fully deuterated, in the presence of 13-(S)-hydroxyoctadecadienoic acid Homo sapiens
1.13.11.33 0.017
-
 linoleic acid fully deuterated Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.33 0.00005
-
 arachidonic acid at 15°C, normalized to iron content Homo sapiens
1.13.11.33 0.00015
-
 arachidonic acid at 22°C, normalized to iron content Homo sapiens
1.13.11.33 0.00015
-
 arachidonic acid at pH 7.5 Homo sapiens
1.13.11.33 0.00024
-
 arachidonic acid at pH 7 Homo sapiens
1.13.11.33 0.00028
-
 arachidonic acid at pH 8 Homo sapiens
1.13.11.33 0.00046
-
 arachidonic acid at 30°C, normalized to iron content Homo sapiens
1.13.11.33 0.00075
-
 arachidonic acid at 37°C, normalized to iron content Homo sapiens