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Literature summary extracted from

  • Coffin, S.R.; Reich, N.O.
    Escherichia coli DNA adenine methyltransferase: intrasite processivity and substrate-induced dimerization and activation (2009), Biochemistry, 48, 7399-7410.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.72 expressed in Escherichia coli XL2-Blue cells Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 additional information Escherichia coli the preferred substrate consists of the annealed product of oligonucleotides 5'-CATTTACTTGATCCGGTATGC-3' and 5'-GCATACCGGATCAAGTAAATG-3', while the nonpreferred substrate consists of the annealed product of oligonucleotides 5'-CATTTAGACGATCTTTTATGC-3' and 5'-GCATAAAAGATCGTCTAAATG-3' ?
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Escherichia coli Dam belongs to the alpha-class of adenine methyltransferases and transfers a methyl group to the N-6 position of the adenine in the DNA sequence 5'-GATC-3' S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.72 phosphocellulose column chromatography and Blue Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 additional information the preferred substrate consists of the annealed product of oligonucleotides 5'-CATTTACTTGATCCGGTATGC-3' and 5'-GCATACCGGATCAAGTAAATG-3', while the nonpreferred substrate consists of the annealed product of oligonucleotides 5'-CATTTAGACGATCTTTTATGC-3' and 5'-GCATAAAAGATCGTCTAAATG-3' Escherichia coli ?
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Dam belongs to the alpha-class of adenine methyltransferases and transfers a methyl group to the N-6 position of the adenine in the DNA sequence 5'-GATC-3' Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.72 dimer Dam dimerizes on short, synthetic DNA, resulting in enhanced catalysis, however, dimerization is not observed on large genomic DNA Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 Dam
-
 Escherichia coli
2.1.1.72 DNA adenine methyltransferase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.72 0.003
-
 DNA adenine in 100 mM Tris (pH 8.0), 1 mM EDTA, 1 mM dithiothreitol, and 0.2 mg/ml bovine serum albumin, at 22°C Escherichia coli

General Information

EC Number General Information Comment Organism
2.1.1.72 physiological function DNA adenine methyltransferase is essential for proper DNA replication timing, gene regulation, and mismatch repair Escherichia coli