EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.33 | 12(S)-hydroperoxyeicosatetraenoic acid | reduces the kinetic lag phase | Homo sapiens | |
1.13.11.33 | 13(S)-hydroperoxyoctadecadienoic acid | activates 15-hLO-1 and removes the kinetic lag phase | Homo sapiens | |
1.13.11.33 | 15(S)-hydroperoxyeicosatetraenoic acid | activates 15-hLO-1 and removes the kinetic lag phase | Homo sapiens | |
1.13.11.33 | additional information | under cellular conditions (low fatty acid and low oxygen concentrations), the allosteric binding of 12(S)-hydroxyeicosatetraenoic acid to 15-hLO-1 can increase the substrate specificity of 15-hLO-1 toward arachidonic acid over linoleic acid significantly, tunneling mechanism | Homo sapiens |
EC Number | Application | Comment | Organism |
---|---|---|---|
1.13.11.33 | medicine | under cellular conditions (low fatty acid and low oxygen concentrations), the allosteric binding of 12(S)-hydroxyeicosatetraenoic acid to 15-hLO-1 can increase the substrate specificity of 15-hLO-1 toward arachidonic acid over linoleic acid significantlysignificantly, which may have important implications in cancer progression | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.31 | overexpressed as N-terminally, His6-tagged protein | Homo sapiens |
1.13.11.33 | overexpressed as N-terminally, His6-tagged protein | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.33 | arachidonic acid | autoinactivates 15-hLO-1 to a much greater extent than linoleic acid at high substrate concentrations. No autoinactivation at low substrate concentrations | Homo sapiens | |
1.13.11.33 | linoleic acid | autoinactivates 15-hLO-1 to a much greater extent than linoleic acid at high substrate concentrations. No autoinactivation at low substrate concentrations | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.33 | 0.0096 | - |
O2 | with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C | Homo sapiens | |
1.13.11.33 | 0.015 | - |
O2 | with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid | Homo sapiens | |
1.13.11.33 | 0.022 | - |
O2 | with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid | Homo sapiens | |
1.13.11.33 | 0.024 | - |
O2 | with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.31 | Fe2+ | - |
Homo sapiens | |
1.13.11.33 | Fe2+ | iron-containing metalloenzyme | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.31 | Homo sapiens | - |
- |
- |
1.13.11.33 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.31 | to greater than 90% purity | Homo sapiens |
1.13.11.33 | to greater than 90% purity | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.13.11.31 | blood platelet | - |
Homo sapiens | - |
1.13.11.33 | reticulocyte | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.31 | arachidonate + O2 | - |
Homo sapiens | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate | - |
? | |
1.13.11.33 | arachidonate + O2 | 5fold higher affinity for arachidonic acid than for linoleic acid for 15-hLO-1 | Homo sapiens | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | - |
? | |
1.13.11.33 | linoleic acid + O2 | - |
Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.31 | 12-lipoxygenase | - |
Homo sapiens |
1.13.11.31 | 12-LO | - |
Homo sapiens |
1.13.11.33 | 15-hLO-1 | - |
Homo sapiens |
1.13.11.33 | 15-lipoxygenase-1 | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.33 | 30 | - |
- |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.31 | 1.4 | - |
arachidonic acid | at 15°C, pH 7.5 | Homo sapiens | |
1.13.11.31 | 20 | - |
arachidonic acid | at 40°C, pH 7.5 | Homo sapiens | |
1.13.11.33 | 5.1 | - |
O2 | with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid | Homo sapiens | |
1.13.11.33 | 5.6 | - |
O2 | with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C | Homo sapiens | |
1.13.11.33 | 7 | - |
O2 | with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid | Homo sapiens | |
1.13.11.33 | 7.6 | - |
O2 | with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.31 | 1.4 | - |
arachidonic acid | at 15°C, pH 7.5 | Homo sapiens | |
1.13.11.31 | 50 | - |
arachidonic acid | at 40°C, pH 7.5 | Homo sapiens | |
1.13.11.33 | 240 | - |
O2 | with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C | Homo sapiens | |
1.13.11.33 | 320 | - |
O2 | with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid | Homo sapiens | |
1.13.11.33 | 340 | - |
O2 | with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid | Homo sapiens | |
1.13.11.33 | 790 | - |
O2 | with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C | Homo sapiens |