Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pochapsky, S.S.; Dang, M.; OuYang, B.; Simorellis, A.K.; Pochapsky, T.C.
    Redox-dependent dynamics in cytochrome P450cam (2009), Biochemistry, 48, 4254-4261.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.1 mutant C334A transformed into chemically competent Escherichia coli NCM533 cells Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.1 C334A is identical spectroscopically and enzymatically to wild-type but does not dimerize in solution at high concentrations Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.1 Fe2+ local protein backbone dynamics of CYP101 depend upon the oxidation and ligation state of the heme iron Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.1 by gel filtration Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-camphor + O2 + reduced putidaredoxin the reduced enzyme exhibits lower-amplitude motions of secondary structural features than the oxidized enzyme on all of the time scales accessible, and these differences are more pronounced in regions of the enzyme involved in substrate access to the active site (B' helix and beta3 and beta5 sheets) and binding of putidaredoxin (C and L helices), the iron-sulfur protein that acts as the effector and reductant of CYP101 in vivo Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.1 camphor hydroxylase cytochrome P450cam
-
 Pseudomonas putida
1.14.15.1 CYP101
-
 Pseudomonas putida
1.14.15.1 P450cam
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.1 putidaredoxin
-
 Pseudomonas putida