Literature summary extracted from

Brandt, G.S.; Kneen, M.M.; Chakraborty, S.; Baykal, A.T.; Nemeria, N.; Yep, A.; Ruby, D.I.; Petsko, G.A.; Kenyon, G.L.; McLeish, M.J.; Jordan, F.; Ringe, D.
Snapshot of a reaction intermediate: analysis of benzoylformate decarboxylase in complex with a benzoylphosphonate inhibitor (2009), Biochemistry, 48, 3247-3257.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
4.1.1.7	in complex with methyl benzoylphosphonate, hanging drop vapor diffusion method, using 100 mM Tris-HCl (pH 8.5), 150 mM CaCl2, 22% (v/v) PEG 400	Pseudomonas putida

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
4.1.1.7	benzoylphosphonate	the reaction of methyl benzoylphosphonate with BFDC results in the formation of a stable analogue (C2alpha-phosphonomandelyl thiamine diphosphate) of the covalent thiamine diphosphate-substrate adduct C2alpha-mandelyl thiamine diphosphate	Pseudomonas putida
4.1.1.7	methyl benzoylphosphonate	competitive inhibitor	Pseudomonas putida

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.1.1.7	0.76	-	methyl benzoylphosphonate	-	Pseudomonas putida

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.1.1.7	Pseudomonas putida	P20906	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.1.1.7	benzaldehyde + acetaldehyde	-	Pseudomonas putida	(S)-2-hydroxypropiophenone	-	r
4.1.1.7	benzaldehyde + benzaldehyde	-	Pseudomonas putida	(R)-benzoin	-	r
4.1.1.7	methyl benzoylphosphonate	-	Pseudomonas putida	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
4.1.1.7	BFDC	-	Pseudomonas putida

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
4.1.1.7	thiamine diphosphate	-	Pseudomonas putida

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
4.1.1.7	0.38	-	methyl benzoylphosphonate	-	Pseudomonas putida

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Release 2023.1 (January 2023)