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Literature summary extracted from

  • Liggins, J.R.; Gready, J.E.
    Putative functional role for the invariant aspartate 263 residue of Rhodospirillum rubrum Rubisco (2009), Biochemistry, 48, 2226-2236.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.39 expressed in Escherichia coli as a His-tagged fusion protein Rhodospirillum rubrum

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.39 D263A kcat and Km (substrate: CO2) (substrate: D-ribulose 1,5-bisphosphate) are reduced compared to wildtype Rhodospirillum rubrum
4.1.1.39 D263E kcat and Km (substrate: CO2) (substrate: D-ribulose 1,5-bisphosphate) are reduced compared to wildtype, mutant D263E has a significantly higher Km value for (substrate: CO2) compared to mutant D263N (although both mutants have approximately the same kcat value) Rhodospirillum rubrum
4.1.1.39 D263N kcat and Km (substrate: CO2) (substrate: D-ribulose 1,5-bisphosphate) are reduced compared to wildtype, mutant D263N has a significantly lower Km value for (substrate: CO2) compared to mutant D263N (although both mutants have approximately the same kcat value) Rhodospirillum rubrum
4.1.1.39 D263Q D263Q shows the lowest value for kcat and Km (substrate: CO2) (substrate: D-ribulose 1,5-bisphosphate) Rhodospirillum rubrum
4.1.1.39 D263S kcat and Km (substrate: CO2) (substrate: D-ribulose 1,5-bisphosphate) are reduced compared to wildtype Rhodospirillum rubrum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.39 0.0013
-
 D-ribulose 1,5-bisphosphate mutant D263Q Rhodospirillum rubrum
4.1.1.39 0.0042
-
 D-ribulose 1,5-bisphosphate mutant D263E Rhodospirillum rubrum
4.1.1.39 0.005
-
 D-ribulose 1,5-bisphosphate mutant D263A Rhodospirillum rubrum
4.1.1.39 0.0089
-
 D-ribulose 1,5-bisphosphate mutant D263S Rhodospirillum rubrum
4.1.1.39 0.009
-
 CO2 mutant D263S Rhodospirillum rubrum
4.1.1.39 0.0098
-
 D-ribulose 1,5-bisphosphate mutant D263N Rhodospirillum rubrum
4.1.1.39 0.0128
-
 D-ribulose 1,5-bisphosphate wild-type Rhodospirillum rubrum
4.1.1.39 0.014
-
 CO2 mutant D263Q Rhodospirillum rubrum
4.1.1.39 0.018
-
 CO2 mutant D263A Rhodospirillum rubrum
4.1.1.39 0.026
-
 CO2 mutant D263N Rhodospirillum rubrum
4.1.1.39 0.076
-
 CO2 mutant D263E Rhodospirillum rubrum
4.1.1.39 0.446
-
 CO2 wild-type Rhodospirillum rubrum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.39 Mg2+
-
 Rhodospirillum rubrum

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.39 Rhodospirillum rubrum P04718
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.39 using Ni-NTA chromatography Rhodospirillum rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.39 D-Ribulose 1,5-bisphosphate + CO2
-
 Rhodospirillum rubrum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.39 Ribulose 1,5-bisphosphate carboxylase
-
 Rhodospirillum rubrum
4.1.1.39 Rubisco
-
 Rhodospirillum rubrum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.39 25
-
 assay at Rhodospirillum rubrum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.39 0.2
-
 D-ribulose 1,5-bisphosphate mutant D263Q, cosubstrate: CO2 Rhodospirillum rubrum
4.1.1.39 0.2
-
 CO2 mutant D263Q, cosubstrate: D-ribulose 1,5-bisphosphate Rhodospirillum rubrum
4.1.1.39 0.46
-
 D-ribulose 1,5-bisphosphate mutant D263S, cosubstrate: CO2 Rhodospirillum rubrum
4.1.1.39 0.46
-
 CO2 mutant D263S, cosubstrate: D-ribulose 1,5-bisphosphate Rhodospirillum rubrum
4.1.1.39 0.81
-
 D-ribulose 1,5-bisphosphate mutant D263A, cosubstrate: CO2 Rhodospirillum rubrum
4.1.1.39 0.81
-
 CO2 mutant D263A, cosubstrate: D-ribulose 1,5-bisphosphate Rhodospirillum rubrum
4.1.1.39 0.82
-
 D-ribulose 1,5-bisphosphate mutant D263N, cosubstrate: CO2 Rhodospirillum rubrum
4.1.1.39 0.82
-
 CO2 mutant D263N, cosubstrate: D-ribulose 1,5-bisphosphate Rhodospirillum rubrum
4.1.1.39 0.94
-
 D-ribulose 1,5-bisphosphate mutant D263E, cosubstrate: CO2 Rhodospirillum rubrum
4.1.1.39 0.94
-
 CO2 mutant D263E, cosubstrate: D-ribulose 1,5-bisphosphate Rhodospirillum rubrum
4.1.1.39 6.28
-
 D-ribulose 1,5-bisphosphate wild-type, cosubstrate: CO2 Rhodospirillum rubrum
4.1.1.39 6.28
-
 CO2 wild-type, cosubstrate: D-ribulose 1,5-bisphosphate Rhodospirillum rubrum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.39 8
-
 assay at Rhodospirillum rubrum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.39 12
-
 CO2 mutant D263E Rhodospirillum rubrum
4.1.1.39 14
-
 CO2 wild-type Rhodospirillum rubrum
4.1.1.39 15
-
 CO2 mutant D263Q Rhodospirillum rubrum
4.1.1.39 32
-
 CO2 mutant D263N Rhodospirillum rubrum
4.1.1.39 44
-
 CO2 mutant D263A Rhodospirillum rubrum
4.1.1.39 49
-
 CO2 mutant D263S Rhodospirillum rubrum
4.1.1.39 52
-
 D-ribulose 1,5-bisphosphate mutant D263S Rhodospirillum rubrum
4.1.1.39 84
-
 D-ribulose 1,5-bisphosphate mutant D263N Rhodospirillum rubrum
4.1.1.39 160
-
 D-ribulose 1,5-bisphosphate mutant D263A Rhodospirillum rubrum
4.1.1.39 160
-
 D-ribulose 1,5-bisphosphate mutant D263Q Rhodospirillum rubrum
4.1.1.39 220
-
 D-ribulose 1,5-bisphosphate mutant D263E Rhodospirillum rubrum
4.1.1.39 490
-
 D-ribulose 1,5-bisphosphate wild-type Rhodospirillum rubrum