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Literature summary extracted from

  • Dai, J.; Finci, L.; Zhang, C.; Lahiri, S.; Zhang, G.; Peisach, E.; Allen, K.N.; Dunaway-Mariano, D.
    Analysis of the structural determinants underlying discrimination between substrate and solvent in beta-phosphoglucomutase catalysis (2009), Biochemistry, 48, 1984-1995.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.11
-
 Neisseria meningitidis
3.1.3.11
-
 Lactobacillus delbrueckii subsp. lactis
5.4.2.6
-
 Neisseria meningitidis
5.4.2.6
-
 Lactobacillus delbrueckii subsp. lactis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.11 T16P mutant, hanging drop vapor diffusion method, using 25% (w/v) PEG, 0.2 M NaCl, and 100 mM bis-Tris (pH 6.0) Lactobacillus delbrueckii subsp. lactis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.11 D10A inactive Neisseria meningitidis
3.1.3.11 D10C inactive Lactobacillus delbrueckii subsp. lactis
3.1.3.11 D10E inactive Neisseria meningitidis
3.1.3.11 D10N inactive Lactobacillus delbrueckii subsp. lactis
3.1.3.11 D10S inactive Lactobacillus delbrueckii subsp. lactis
3.1.3.11 H20A the mutant shows reduced activity compared to the wild type enzyme Lactobacillus delbrueckii subsp. lactis
3.1.3.11 H20N the mutant shows reduced activity compared to the wild type enzyme Lactobacillus delbrueckii subsp. lactis
3.1.3.11 H20Q the mutant shows reduced activity compared to the wild type enzyme Lactobacillus delbrueckii subsp. lactis
3.1.3.11 K76A the mutant shows reduced activity compared to the wild type enzyme Lactobacillus delbrueckii subsp. lactis
3.1.3.11 T16P the mutant shows reduced activity compared to the wild type enzyme Lactobacillus delbrueckii subsp. lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 0.0026
-
 beta-D-glucose 1,6-bisphosphate wild type enzyme, in 50 mM K+HEPES pH 7.0 at 25°C Neisseria meningitidis
3.1.3.11 0.0048
-
 beta-D-glucose 1,6-bisphosphate mutant enzyme T16P, in 50 mM K+HEPES pH 7.0 at 25°C Lactobacillus delbrueckii subsp. lactis
3.1.3.11 0.031
-
 beta-D-glucose 1,6-bisphosphate wild type enzyme, in 50 mM K+HEPES pH 7.0 at 25°C Lactobacillus delbrueckii subsp. lactis
3.1.3.11 0.041
-
 beta-D-glucose 1,6-bisphosphate mutant enzyme H20A, in 50 mM K+HEPES pH 7.0 at 25°C Lactobacillus delbrueckii subsp. lactis
3.1.3.11 0.045
-
 beta-D-glucose 1,6-bisphosphate mutant enzyme H20Q, in 50 mM K+HEPES pH 7.0 at 25°C Lactobacillus delbrueckii subsp. lactis
3.1.3.11 0.066
-
 beta-D-glucose 1,6-bisphosphate mutant enzyme K76A, in 50 mM K+HEPES pH 7.0 at 25°C Lactobacillus delbrueckii subsp. lactis
3.1.3.11 0.17
-
 beta-D-glucose 1,6-bisphosphate mutant enzyme H20N, in 50 mM K+HEPES pH 7.0 at 25°C Lactobacillus delbrueckii subsp. lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.11 Mg2+ required Neisseria meningitidis
3.1.3.11 Mg2+ required Lactobacillus delbrueckii subsp. lactis
5.4.2.6 Mg2+ required Neisseria meningitidis
5.4.2.6 Mg2+ required Lactobacillus delbrueckii subsp. lactis

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Lactobacillus delbrueckii subsp. lactis
-
-
-
3.1.3.11 Neisseria meningitidis
-
-
-
5.4.2.6 Lactobacillus delbrueckii subsp. lactis
-
-
-
5.4.2.6 Neisseria meningitidis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11
-
 Neisseria meningitidis
3.1.3.11
-
 Lactobacillus delbrueckii subsp. lactis
5.4.2.6
-
 Neisseria meningitidis
5.4.2.6
-
 Lactobacillus delbrueckii subsp. lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 beta-D-glucose 1,6-bisphosphate + H2O
-
 Neisseria meningitidis D-glucose 6-phosphate + phosphate
-
 ?
3.1.3.11 beta-D-glucose 1,6-bisphosphate + H2O
-
 Lactobacillus delbrueckii subsp. lactis D-glucose 6-phosphate + phosphate
-
 ?
5.4.2.6 beta-D-Glucose 1-phosphate
-
 Neisseria meningitidis beta-D-Glucose 6-phosphate
-
 ?
5.4.2.6 beta-D-Glucose 1-phosphate
-
 Lactobacillus delbrueckii subsp. lactis beta-D-Glucose 6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.11 beta-PGM
-
 Neisseria meningitidis
3.1.3.11 beta-PGM
-
 Lactobacillus delbrueckii subsp. lactis
5.4.2.6 beta-PGM
-
 Neisseria meningitidis
5.4.2.6 beta-PGM
-
 Lactobacillus delbrueckii subsp. lactis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.11 0.026
-
 beta-D-glucose 1,6-bisphosphate mutant enzyme H20A, in 50 mM K+HEPES pH 7.0 at 25°C Lactobacillus delbrueckii subsp. lactis
3.1.3.11 0.026
-
 beta-D-glucose 1,6-bisphosphate mutant enzyme T16P, in 50 mM K+HEPES pH 7.0 at 25°C Lactobacillus delbrueckii subsp. lactis
3.1.3.11 0.62
-
 beta-D-glucose 1,6-bisphosphate mutant enzyme H20N, in 50 mM K+HEPES pH 7.0 at 25°C Lactobacillus delbrueckii subsp. lactis
3.1.3.11 1.56
-
 beta-D-glucose 1,6-bisphosphate mutant enzyme K76A, in 50 mM K+HEPES pH 7.0 at 25°C Lactobacillus delbrueckii subsp. lactis
3.1.3.11 20
-
 beta-D-glucose 1,6-bisphosphate wild type enzyme, in 50 mM K+HEPES pH 7.0 at 25°C Neisseria meningitidis
3.1.3.11 21.9
-
 beta-D-glucose 1,6-bisphosphate mutant enzyme H20Q, in 50 mM K+HEPES pH 7.0 at 25°C Lactobacillus delbrueckii subsp. lactis
3.1.3.11 175
-
 beta-D-glucose 1,6-bisphosphate wild type enzyme, in 50 mM K+HEPES pH 7.0 at 25°C Lactobacillus delbrueckii subsp. lactis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.11 0.6
-
 beta-D-glucose 1,6-bisphosphate mutant enzyme H20A, in 50 mM K+HEPES pH 7.0 at 25°C Lactobacillus delbrueckii subsp. lactis
3.1.3.11 4
-
 beta-D-glucose 1,6-bisphosphate mutant enzyme H20N, in 50 mM K+HEPES pH 7.0 at 25°C Lactobacillus delbrueckii subsp. lactis
3.1.3.11 5
-
 beta-D-glucose 1,6-bisphosphate mutant enzyme T16P, in 50 mM K+HEPES pH 7.0 at 25°C Lactobacillus delbrueckii subsp. lactis
3.1.3.11 20
-
 beta-D-glucose 1,6-bisphosphate mutant enzyme K76A, in 50 mM K+HEPES pH 7.0 at 25°C Lactobacillus delbrueckii subsp. lactis
3.1.3.11 500
-
 beta-D-glucose 1,6-bisphosphate mutant enzyme H20Q, in 50 mM K+HEPES pH 7.0 at 25°C Lactobacillus delbrueckii subsp. lactis
3.1.3.11 6000
-
 beta-D-glucose 1,6-bisphosphate wild type enzyme, in 50 mM K+HEPES pH 7.0 at 25°C Lactobacillus delbrueckii subsp. lactis
3.1.3.11 8000
-
 beta-D-glucose 1,6-bisphosphate wild type enzyme, in 50 mM K+HEPES pH 7.0 at 25°C Neisseria meningitidis