Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jackson, S.G.; Zhang, F.; Chindemi, P.; Junop, M.S.; Berti, P.J.
    Evidence of kinetic control of ligand binding and staged product release in MurA (enolpyruvyl UDP-GlcNAc synthase)-catalyzed reactions (2009), Biochemistry, 48, 11715-11723.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.7 overexpression of wild type MurA Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.7 MurA is crystallized in the presence of sodium phosphite and UDP-N-acetylmuramic acid using the hanging drop vapor diffusion method, the MurA cocrystal structure with UDP-N-acetylmuramic acid and phosphite reveals a new staged MurA conformation in which the Arg397 side chain tracked phosphite out of the catalytic site Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.7 fosfomycin enolpyruvyl UDP-GlcNAc synthase is an antimicrobial target that is inhibited by the antibiotic fosfomycin Escherichia coli
2.5.1.7 UDP-N-acetylmuramic acid
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine Escherichia coli
-
 phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.7 Escherichia coli P0A749
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.7 on to a HiTrap QP-Sepharose HP ion exchange column Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
 Escherichia coli phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.7 monomer it appears to be a monomer in solution, even at high concentrations Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.5.1.7 enolpyruvyl UDP-GlcNAc synthase
-
 Escherichia coli
2.5.1.7 MurA
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.5.1.7 physiological function catalyzes the first committed step in peptidoglycan biosynthesis Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.7 520000
-
 additional information MurA-catalyzed breakdown of its tetrahedral intermediate at pH 7.5, second-order rate constant Escherichia coli
2.5.1.7 570000
-
 additional information MurA-catalyzed breakdown of its tetrahedral intermediate at pH 10.0 Escherichia coli
2.5.1.7 6000000
-
 additional information MurA-catalyzed breakdown of its tetrahedral intermediate at pH 5.0 Escherichia coli