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Literature summary extracted from

  • Li, M.; Shen, J.; Liu, X.; Shao, J.; Yi, W.; Chow, C.S.; Wang, P.G.
    Identification of a new alpha1,2-fucosyltransferase involved in O-antigen biosynthesis of Escherichia coli O86:B7 and formation of H-type 3 blood group antigen (2008), Biochemistry, 47, 11590-11597.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.69 expressed as GST fusion protein in Escherichia coli BL21 (DE3) Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.69 additional information To demonstrate that the variable regions determine substrate specificity and to investigate which of the variable regions of R1,2-FucT is responsible, six chimeric R1,2-FucTs are constructed by domain swapping between Escherichia coli FucTs WbsJ and WbwK. Three chimeras are inactive, the other three chimeras have greater substrate spectra than FucT WbwK but lower activity than WbsJ. Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.69 66000
-
 SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.69 Escherichia coli
-
-
-
2.4.1.69 Escherichia coli Q5JBG3
-
-
2.4.1.69 Escherichia coli O86:B7
-
-
-
2.4.1.69 Escherichia coli O86:B7 Q5JBG3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.69
-
 Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.69 0.852
-
 activity of WbwK-GST fusion protein with Galbeta(1-3)GalNAcalpha-O-benzyl as substrate Escherichia coli
2.4.1.69 0.852
-
 activity of WbwK-GST fusion protein with Galbeta1,3GalNAcalpha-O-benzyl as substrate Escherichia coli
2.4.1.69 0.873
-
 activity of WbwK-GST fusion protein with Galbeta(1-3)GalNAcalpha-O-methyl as substrate Escherichia coli
2.4.1.69 0.873
-
 activity of WbwK-GST fusion protein with Galbeta1,3GalNAcalpha-O-methyl as substrate Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.69 GDP-beta-L-fucose + Gal-beta-OR
-
 Escherichia coli GDP + L-Fucalpha(1-2)Galbeta-OR
-
 ir
2.4.1.69 GDP-beta-L-fucose + Gal-beta-OR
-
 Escherichia coli O86:B7 GDP + L-Fucalpha(1-2)Galbeta-OR
-
 ir
2.4.1.69 GDP-beta-L-fucose + Galbeta(1-3)GalNAcalpha-OMe
-
 Escherichia coli GDP + Fucalpha(1-2)Galbeta(1-3)GalNAcalpha-OMe
-
 ir
2.4.1.69 GDP-beta-L-fucose + Galbeta(1-3)GalNAcalpha-OMe
-
 Escherichia coli O86:B7 GDP + Fucalpha(1-2)Galbeta(1-3)GalNAcalpha-OMe
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.4.1.69 alpha1,2-FucT
-
 Escherichia coli
2.4.1.69 alpha1,2fucosyltransferase
-
 Escherichia coli
2.4.1.69 WbsJ
-
 Escherichia coli
2.4.1.69 WbsJ gene encoding an alpha1,2fucosyltransferase in Escherichia coli with a broad substrate specificity Escherichia coli
2.4.1.69 wbwK
-
 Escherichia coli
2.4.1.69 wbwK gene encoding an alpha1,2fucosyltransferase in Escherichia coli which is only active with substrates containing the Galbeta1,3GalNAcalpha-structure (T-antigen) to form H-type 3 blood group antigen (Fucalpha1,2Galbeta1,3GalNAcalpha-OR) but not with Galbeta-OMe Escherichia coli

General Information

EC Number General Information Comment Organism
2.4.1.69 physiological function Some Escherichia coli strains display human blood group antigens, so called O-antigen structures, on their cell surface to evade the immune system of the human host. alpha1,2fucosyltransferase are involved in the biosynthesis of O-antigen structures. Escherichia coli