EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.69 | expressed as GST fusion protein in Escherichia coli BL21 (DE3) | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.69 | additional information | To demonstrate that the variable regions determine substrate specificity and to investigate which of the variable regions of R1,2-FucT is responsible, six chimeric R1,2-FucTs are constructed by domain swapping between Escherichia coli FucTs WbsJ and WbwK. Three chimeras are inactive, the other three chimeras have greater substrate spectra than FucT WbwK but lower activity than WbsJ. | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.69 | 66000 | - |
SDS-PAGE | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.69 | Escherichia coli | - |
- |
- |
2.4.1.69 | Escherichia coli | Q5JBG3 | - |
- |
2.4.1.69 | Escherichia coli O86:B7 | - |
- |
- |
2.4.1.69 | Escherichia coli O86:B7 | Q5JBG3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.69 | - |
Escherichia coli |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.69 | 0.852 | - |
activity of WbwK-GST fusion protein with Galbeta(1-3)GalNAcalpha-O-benzyl as substrate | Escherichia coli |
2.4.1.69 | 0.852 | - |
activity of WbwK-GST fusion protein with Galbeta1,3GalNAcalpha-O-benzyl as substrate | Escherichia coli |
2.4.1.69 | 0.873 | - |
activity of WbwK-GST fusion protein with Galbeta(1-3)GalNAcalpha-O-methyl as substrate | Escherichia coli |
2.4.1.69 | 0.873 | - |
activity of WbwK-GST fusion protein with Galbeta1,3GalNAcalpha-O-methyl as substrate | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.69 | GDP-beta-L-fucose + Gal-beta-OR | - |
Escherichia coli | GDP + L-Fucalpha(1-2)Galbeta-OR | - |
ir | |
2.4.1.69 | GDP-beta-L-fucose + Gal-beta-OR | - |
Escherichia coli O86:B7 | GDP + L-Fucalpha(1-2)Galbeta-OR | - |
ir | |
2.4.1.69 | GDP-beta-L-fucose + Galbeta(1-3)GalNAcalpha-OMe | - |
Escherichia coli | GDP + Fucalpha(1-2)Galbeta(1-3)GalNAcalpha-OMe | - |
ir | |
2.4.1.69 | GDP-beta-L-fucose + Galbeta(1-3)GalNAcalpha-OMe | - |
Escherichia coli O86:B7 | GDP + Fucalpha(1-2)Galbeta(1-3)GalNAcalpha-OMe | - |
ir |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.69 | alpha1,2-FucT | - |
Escherichia coli |
2.4.1.69 | alpha1,2fucosyltransferase | - |
Escherichia coli |
2.4.1.69 | WbsJ | - |
Escherichia coli |
2.4.1.69 | WbsJ | gene encoding an alpha1,2fucosyltransferase in Escherichia coli with a broad substrate specificity | Escherichia coli |
2.4.1.69 | wbwK | - |
Escherichia coli |
2.4.1.69 | wbwK | gene encoding an alpha1,2fucosyltransferase in Escherichia coli which is only active with substrates containing the Galbeta1,3GalNAcalpha-structure (T-antigen) to form H-type 3 blood group antigen (Fucalpha1,2Galbeta1,3GalNAcalpha-OR) but not with Galbeta-OMe | Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.1.69 | physiological function | Some Escherichia coli strains display human blood group antigens, so called O-antigen structures, on their cell surface to evade the immune system of the human host. alpha1,2fucosyltransferase are involved in the biosynthesis of O-antigen structures. | Escherichia coli |