BRENDA - Enzyme Database

Identification of two Escherichia coli pseudouridine synthases that show multisite specificity for 23S RNA

Huang, L.; Ku, J.; Pookanjanatavip, M.; Gu, X.; Wang, D.; Greene, P.J.; Santi, D.V.; Biochemistry 37, 15951-15957 (1998)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.4.99.23
overexpressed in Escherichia coli
Escherichia coli
5.4.99.24
overexpressed in Escherichia coli
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.99.23
23S rRNA uridine1911/uridine1915/uridine1917
Escherichia coli
-
23S rRNA pseudouridine1911/pseudouridine1915/pseudouridine1917
-
-
?
5.4.99.24
23S rRNA uridine955/uridine2504/uridine2580
Escherichia coli
-
23S rRNA pseudouridine955/pseudouridine2504/pseudouridine2580
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.99.23
Escherichia coli
-
-
-
5.4.99.24
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.99.23
-
Escherichia coli
5.4.99.24
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.23
23S rRNA uridine1911/uridine1915/uridine1917
-
702195
Escherichia coli
23S rRNA pseudouridine1911/pseudouridine1915/pseudouridine1917
-
-
-
?
5.4.99.24
23S rRNA uridine955/uridine2504/uridine2580
-
702195
Escherichia coli
23S rRNA pseudouridine955/pseudouridine2504/pseudouridine2580
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.99.23
overexpressed in Escherichia coli
Escherichia coli
5.4.99.24
overexpressed in Escherichia coli
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.99.23
23S rRNA uridine1911/uridine1915/uridine1917
Escherichia coli
-
23S rRNA pseudouridine1911/pseudouridine1915/pseudouridine1917
-
-
?
5.4.99.24
23S rRNA uridine955/uridine2504/uridine2580
Escherichia coli
-
23S rRNA pseudouridine955/pseudouridine2504/pseudouridine2580
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.99.23
-
Escherichia coli
5.4.99.24
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.23
23S rRNA uridine1911/uridine1915/uridine1917
-
702195
Escherichia coli
23S rRNA pseudouridine1911/pseudouridine1915/pseudouridine1917
-
-
-
?
5.4.99.24
23S rRNA uridine955/uridine2504/uridine2580
-
702195
Escherichia coli
23S rRNA pseudouridine955/pseudouridine2504/pseudouridine2580
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
5.4.99.23
malfunction
yfiI-disrupted cells show a dramatic decrease in growth rate
Escherichia coli
5.4.99.24
malfunction
disruption of yceC does not affect the growth
Escherichia coli
General Information (protein specific)
EC Number
General Information
Commentary
Organism
5.4.99.23
malfunction
yfiI-disrupted cells show a dramatic decrease in growth rate
Escherichia coli
5.4.99.24
malfunction
disruption of yceC does not affect the growth
Escherichia coli