BRENDA - Enzyme Database

Purification, cloning, and properties of the 16S RNA pseudouridine 516 synthase from Escherichia coli

Wrzesinski, J.; Bakin, A.; Nurse, K.; Lane, B.G.; Ofengand, J.; Biochemistry 34, 8904-8913 (1995)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.4.99.19
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.99.19
25836
-
x * 25836, calculated from sequence
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.99.19
16S rRNA uridine516
Escherichia coli
in vitro, the enzyme does not modify free 16S rRNA. The preferred substrate is a 5'-terminal fragment of 16S rRNA complexed with 30S ribosomal proteins, suggesting that pseudouridylation occurs at an intermediate stage of 30S assembly in vivo
16S rRNA pseudouridine516
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.99.19
Escherichia coli
P0AA43
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.99.19
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.19
16S rRNA uridine516
in vitro, the enzyme does not modify free 16S rRNA. The preferred substrate is a 5'-terminal fragment of 16S rRNA complexed with 30S ribosomal proteins, suggesting that pseudouridylation occurs at an intermediate stage of 30S assembly in vivo
702191
Escherichia coli
16S rRNA pseudouridine516
-
-
-
?
5.4.99.19
16S rRNA uridine516
the enzyme is specific for uridine516 in 16S rRNA. In vitro, the enzyme does not modify free 16S rRNA. The preferred substrate is a 5'-terminal fragment of 16S rRNA complexed with 30S ribosomal proteins
702191
Escherichia coli
16S rRNA pseudouridine516
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
5.4.99.19
?
x * 25836, calculated from sequence
Escherichia coli
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
5.4.99.19
37
-
assay at
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.99.19
7.5
-
assay at
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.99.19
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.99.19
25836
-
x * 25836, calculated from sequence
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.99.19
16S rRNA uridine516
Escherichia coli
in vitro, the enzyme does not modify free 16S rRNA. The preferred substrate is a 5'-terminal fragment of 16S rRNA complexed with 30S ribosomal proteins, suggesting that pseudouridylation occurs at an intermediate stage of 30S assembly in vivo
16S rRNA pseudouridine516
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.99.19
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.19
16S rRNA uridine516
in vitro, the enzyme does not modify free 16S rRNA. The preferred substrate is a 5'-terminal fragment of 16S rRNA complexed with 30S ribosomal proteins, suggesting that pseudouridylation occurs at an intermediate stage of 30S assembly in vivo
702191
Escherichia coli
16S rRNA pseudouridine516
-
-
-
?
5.4.99.19
16S rRNA uridine516
the enzyme is specific for uridine516 in 16S rRNA. In vitro, the enzyme does not modify free 16S rRNA. The preferred substrate is a 5'-terminal fragment of 16S rRNA complexed with 30S ribosomal proteins
702191
Escherichia coli
16S rRNA pseudouridine516
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.99.19
?
x * 25836, calculated from sequence
Escherichia coli
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
5.4.99.19
37
-
assay at
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.99.19
7.5
-
assay at
Escherichia coli