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Literature summary extracted from

  • Lu, S.Y.; Zhao, G.R.; Wu, A.M.; Jenks, M.A.; Zhang, S.; Liu, J.Y.
    Molecular cloning of a cotton phosphatase gene and its functional characterization (2010), Biochemistry (Moscow), 75, 85-94.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.3.7 biotechnology GhHL1 is a functional and good candidate gene that might be used to improve salt tolerance in plants Gossypium hirsutum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.7 GhHL1 gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21, overexpression of GhHL1 complements yeast hal2 mutant and enhances yeast growth under elevated NaCl or LiCl Gossypium hirsutum
3.1.3.57 His-tagged version expressed in Escherichia coli BL21(DE3), expressed in Saccharomyces cerevisiae JRM4 Gossypium hirsutum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.57 Ca2+
-
 Gossypium hirsutum
3.1.3.57 K+
-
 Gossypium hirsutum
3.1.3.57 lithium ion
-
 Gossypium hirsutum
3.1.3.57 Na+
-
 Gossypium hirsutum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.57 additional information
-
 3'-phosphoadenosine 5'-phosphosulfate KM value lower than 0.01 mM Gossypium hirsutum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.7 Mg2+ dependent on, optimal at 2 mM Gossypium hirsutum
3.1.3.7 additional information GhHL1 is Mg2+-dependent and cation-sensitive Gossypium hirsutum
3.1.3.57 Mg2+ optimal concentration 2mM, 3',5'-bisphosphate nucleotide as substrate Gossypium hirsutum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.7 37000
-
 x * 37000, recombinant enzyme, SDS-PAGE Gossypium hirsutum
3.1.3.57 37000
-
 x * 37000, SDS-PAGE Gossypium hirsutum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.7 Gossypium hirsutum Q8VWZ6
-
-
3.1.3.7 Gossypium hirsutum CRI 12 Q8VWZ6
-
-
3.1.3.57 Gossypium hirsutum Q8VWZ6 cv. CRI 121
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.57 immobilized metal ion affinity chromatography (Ni2+) Gossypium hirsutum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.7 adenosine 3',5'-bisphosphate + H2O
-
 Gossypium hirsutum adenosine 5'-phosphate + phosphate
-
 ?
3.1.3.7 adenosine 3',5'-bisphosphate + H2O
-
 Gossypium hirsutum CRI 12 adenosine 5'-phosphate + phosphate
-
 ?
3.1.3.7 adenosine 3',5'-bisphosphate sulfate + H2O
-
 Gossypium hirsutum adenosine 5'-phosphate sulfate + phosphate
-
 ?
3.1.3.7 adenosine 3',5'-bisphosphate sulfate + H2O
-
 Gossypium hirsutum CRI 12 adenosine 5'-phosphate sulfate + phosphate
-
 ?
3.1.3.7 additional information GhHL1 protein efficiently hydrolyzes 3'(2'),5'-bisphosphate nucleotide and 3'(2'),5'-bisphosphate nucleotide sulfate, at 128% of 3'(2'),5'-bisphosphate nucleotide levels, but has low or no activity with other tested compounds including 3'-AMP, 5'-AMP, or ADP. Purified recombinant GhHL1 protein dephosphorylates both 3',5'-bisphosphate nucleotide and inositol 1,4-bisphosphate, demonstrating dual 3',5'-bisphosphate nucleotidase and inositol polyphosphate 1-phosphatase activities, substrate specificity, overview Gossypium hirsutum ?
-
 ?
3.1.3.7 additional information GhHL1 protein efficiently hydrolyzes 3'(2'),5'-bisphosphate nucleotide and 3'(2'),5'-bisphosphate nucleotide sulfate, at 128% of 3'(2'),5'-bisphosphate nucleotide levels, but has low or no activity with other tested compounds including 3'-AMP, 5'-AMP, or ADP. Purified recombinant GhHL1 protein dephosphorylates both 3',5'-bisphosphate nucleotide and inositol 1,4-bisphosphate, demonstrating dual 3',5'-bisphosphate nucleotidase and inositol polyphosphate 1-phosphatase activities, substrate specificity, overview Gossypium hirsutum CRI 12 ?
-
 ?
3.1.3.57 3'-phosphoadenosine 5'-phosphosulfate + H2O
-
 Gossypium hirsutum ?
-
 ?
3.1.3.57 3'-phosphoadenosine 5'-phosphosulfate + H2O
-
 Gossypium hirsutum CRI 12 ?
-
 ?
3.1.3.57 3'-phosphoadenosine 5'-phosphosulfate + H2O 128% of activity observed with 3'(2'),5'-bisphosphate nucleotide Gossypium hirsutum adenosine 5'-phosphosulfate + phosphate
-
 ?
3.1.3.57 adenosine 3',5'-bisphosphate + H2O
-
 Gossypium hirsutum adenosine 5'-phosphate + phosphate
-
 ?
3.1.3.57 adenosine 3',5'-bisphosphate + H2O
-
 Gossypium hirsutum CRI 12 adenosine 5'-phosphate + phosphate
-
 ?
3.1.3.57 inositol 1,4,5-trisphosphate + H2O 4.58% of activity observed with 3'(2'),5'-bisphosphate nucleotide Gossypium hirsutum ? + phosphate
-
 ?
3.1.3.57 inositol 1,4-bisphosphate + H2O 35% of activity observed with adenosine 3',5'-bisphosphate Gossypium hirsutum ? + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.7 ? x * 37000, recombinant enzyme, SDS-PAGE Gossypium hirsutum
3.1.3.57 ? x * 37000, SDS-PAGE Gossypium hirsutum

Synonyms

EC Number Synonyms Comment Organism
3.1.3.7 GhHL1
-
 Gossypium hirsutum
3.1.3.57 inositol polyphosphate 1-phosphatase dual activities: 3',5'-bisphosphate nucleotidase Gossypium hirsutum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.7 30
-
-
 Gossypium hirsutum
3.1.3.57 30 60 pH 7.5, 2 mM Mg2+, adenosine 3',5'-bisphosphate as substrate Gossypium hirsutum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.7 10 75 highest activity at 30-65°C, low activity at below 10°C, and 28.9% of maximal activity at 75°C Gossypium hirsutum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.7 7.5
-
-
 Gossypium hirsutum
3.1.3.57 7.5
-
 30°C, 2 mM Mg2+, adenosine 3',5'-bisphosphate as substrate Gossypium hirsutum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.7 6.5 9 activity range, highest activity in the pH range from pH 7.0 to pH 8.5 Gossypium hirsutum
3.1.3.57 6.5 9 adenosine 3',5'-bisphosphate as substrate Gossypium hirsutum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.57 0.01
-
 30°C, pH 7.5, 0.55 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate Gossypium hirsutum Ca2+
3.1.3.57 0.05
-
 30°C, pH 7.5, 2 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate Gossypium hirsutum Ca2+
3.1.3.57 0.2
-
 30°C, pH 7.5, 2 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate Gossypium hirsutum lithium ion
3.1.3.57 0.8
-
 30°C, pH 7.5, 5 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate Gossypium hirsutum Ca2+
3.1.3.57 100
-
 30°C, pH 7.5, 2 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate Gossypium hirsutum Na+
3.1.3.57 200
-
 30°C, pH 7.5, 2 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate Gossypium hirsutum K+

General Information

EC Number General Information Comment Organism
3.1.3.7 physiological function overexpression of GhHL1 complements yeast hal2 mutant and enhances yeast growth under elevated NaCl or LiCl, showing a role in salt tolerance associated with ionic stress response Gossypium hirsutum