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Literature summary extracted from

  • Yadav, S.; Yadav, P.K.; Yadav, D.; Yadav, K.D.
    Purification and characterization of pectin lyase secreted by Penicillium citrinum (2009), Biochemistry (Moscow), 74, 800-806.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.10 Sodium arsenate 122% relative activity at 1 mM Penicillium citrinum

Application

EC Number Application Comment Organism
4.2.2.10 additional information the purified pectin lyase is efficient in retting of of natural fibers of mature stems of Linum usitatissimum, Cannabis sativa, and Crotalaria juncea Penicillium citrinum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.10 Ag+ no residual activity at 1 mM Penicillium citrinum
4.2.2.10 Cu2+ 10% residual activity at 1 mM Penicillium citrinum
4.2.2.10 EDTA 97% residual activity at 1 mM Penicillium citrinum
4.2.2.10 Hg2+ 24% residual activity at 1 mM Penicillium citrinum
4.2.2.10 K+ 91% residual activity at 1 mM Penicillium citrinum
4.2.2.10 K4[Fe(CN)6] no residual activity at 1 mM Penicillium citrinum
4.2.2.10 KMnO4 no residual activity at 1 mM Penicillium citrinum
4.2.2.10 Mg2+ 97% residual activity at 1 mM Penicillium citrinum
4.2.2.10 Mn2+ 76% residual activity at 1 mM Penicillium citrinum
4.2.2.10 Na+ 97% residual activity at 1 mM Penicillium citrinum
4.2.2.10 Sodium azide 91% residual activity at 1 mM Penicillium citrinum
4.2.2.10 Zn2+ 76% residual activity at 1 mM Penicillium citrinum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.10 Co2+ 114% relative activity at 1 mM Penicillium citrinum
4.2.2.10 additional information Ca2+ and Co2+ have no significant effects on activity Penicillium citrinum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.10 31000
-
 SDS-PAGE Penicillium citrinum

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.10 Penicillium citrinum
-
-
-
4.2.2.10 Penicillium citrinum MTCC 8897
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.10 Sephadex G 100 gel filtration and DEAE-cellulose column chromatography Penicillium citrinum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.10 1.5
-
 crude extract Penicillium citrinum
4.2.2.10 12.9
-
 after 8.6fold purification Penicillium citrinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.10 pectin
-
 Penicillium citrinum ?
-
 ?
4.2.2.10 pectin
-
 Penicillium citrinum MTCC 8897 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.10 PNL
-
 Penicillium citrinum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.10 50
-
-
 Penicillium citrinum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.10 40
-
 the pectin lyase is completely stable up to 40°C when exposed for 1 h, above 40°C it starts loosing activity Penicillium citrinum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.10 76
-
 Pectin
-
 Penicillium citrinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.10 9
-
 purified pectin lyase Penicillium citrinum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.10 9 12 the enzyme retains activity in the pH 9.0-12.0 range when exposed for 24 h Penicillium citrinum