BRENDA - Enzyme Database

Physicochemical and kinetic characteristics of isoforms of isocitrate lyase from corn

Eprintsev, A.T.; Maslova, E.V.; Fedorin, D.N.; Popov, V.N.; Biochemistry (Moscow) 74, 528-532 (2009)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.056
-
isocitrate
ICL1
Zea mays
4.1.3.1
0.083
-
isocitrate
ICL2
Zea mays
4.1.3.1
0.1
-
isocitrate
ICL
Zea mays
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
ICL1, dependent on
Zea mays
4.1.3.1
Mn2+
ICL2 and ICL, dependent on
Zea mays
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
164000
-
gel filtration, ICL1
Zea mays
4.1.3.1
207000
-
gel filtration, ICL2
Zea mays
4.1.3.1
208000
-
gel filtration, ICL
Zea mays
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Zea mays
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
to homogeneity, purification of ICL1 is 123fold with a yield of 5.7%, purification degree and yield of ICL2 are 110 and 5.7%, respectively
Zea mays
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
leaf
ICL
Zea mays
-
4.1.3.1
scutellum
ICL1 and and ICL2
Zea mays
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.1.3.1
0.04
-
homogenate
Zea mays
4.1.3.1
4.4
-
ICL2, 1003fold purified
Zea mays
4.1.3.1
4.9
-
ICL1, 123fold purified
Zea mays
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
ICL1, ICL2 and ICL
702178
Zea mays
succinate + glyoxylate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
gel filtration, ICL1, ICL2 and ICL
Zea mays
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6
-
ICL2
Zea mays
4.1.3.1
7.5
-
ICL1
Zea mays
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.056
-
isocitrate
ICL1
Zea mays
4.1.3.1
0.083
-
isocitrate
ICL2
Zea mays
4.1.3.1
0.1
-
isocitrate
ICL
Zea mays
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
ICL1, dependent on
Zea mays
4.1.3.1
Mn2+
ICL2 and ICL, dependent on
Zea mays
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
164000
-
gel filtration, ICL1
Zea mays
4.1.3.1
207000
-
gel filtration, ICL2
Zea mays
4.1.3.1
208000
-
gel filtration, ICL
Zea mays
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
to homogeneity, purification of ICL1 is 123fold with a yield of 5.7%, purification degree and yield of ICL2 are 110 and 5.7%, respectively
Zea mays
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
leaf
ICL
Zea mays
-
4.1.3.1
scutellum
ICL1 and and ICL2
Zea mays
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.1.3.1
0.04
-
homogenate
Zea mays
4.1.3.1
4.4
-
ICL2, 1003fold purified
Zea mays
4.1.3.1
4.9
-
ICL1, 123fold purified
Zea mays
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
ICL1, ICL2 and ICL
702178
Zea mays
succinate + glyoxylate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
gel filtration, ICL1, ICL2 and ICL
Zea mays
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6
-
ICL2
Zea mays
4.1.3.1
7.5
-
ICL1
Zea mays
Expression
EC Number
Organism
Commentary
Expression
4.1.3.1
Zea mays
Ca2+, K+, and Na+ have little effect on the regulation of activity of ICL isoforms
additional information
General Information
EC Number
General Information
Commentary
Organism
4.1.3.1
metabolism
participation of isoforms in metabolic regulation of the glyoxylate cycle, organic acid metabolism during photorespiration in leaves and acidosis in corn seeds
Zea mays
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.1.3.1
metabolism
participation of isoforms in metabolic regulation of the glyoxylate cycle, organic acid metabolism during photorespiration in leaves and acidosis in corn seeds
Zea mays
Expression (protein specific)
EC Number
Organism
Commentary
Expression
4.1.3.1
Zea mays
Ca2+, K+, and Na+ have little effect on the regulation of activity of ICL isoforms
additional information