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Literature summary extracted from

  • van Bemmel, D.M.; Brank, A.S.; Eritja, R.; Marquez, V.E.; Christman, J.K.
    DNA (Cytosine-C5) methyltransferase inhibition by oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone (zebularine aglycon) at the enzymatic target site (2009), Biochem. Pharmacol., 78, 633-641.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.37 M.HhaI overexpression in Escherichia coli strain ER1727 Haemophilus parahaemolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.37 2-(1H)-pyrimidinone riboside i.e. zebularine, is a more stable, less cytotoxic inhibitor compared to 5-azacytidine probably due to differing stability and reversibility of the covalent bonds. The ternary complexes between the enzyme, 2-(1H)-pyrimidinone inhibitor, and the cofactor S-adenosyl-L-methionine are maintained through the formation of a reversible covalent interaction Haemophilus parahaemolyticus
2.1.1.37 5-azacytidine zebularine is a more stable, less cytotoxic inhibitor compared to 5-azacytidine probably due to differing stability and reversibility of the covalent bonds Haemophilus parahaemolyticus
2.1.1.37 additional information inhibitor binding kinetics Haemophilus parahaemolyticus
2.1.1.37 oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone at the enzymatic target site are competitive inhibitors of both prokaryotic and mammalian DNA C5 methyltransferases, overview Haemophilus parahaemolyticus
2.1.1.37 oligodeoxyribonucleotides containing 5-azacytidine 5-azacytosine oligodeoxyribonucleotides form complexes with C5 DNA methyltransferases that are irreversible when the 5-azacytosine ring is intact, overview Haemophilus parahaemolyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.37 DNA + S-adenosyl-L-methionine Haemophilus parahaemolyticus
-
 DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.37 Haemophilus parahaemolyticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.37 DNA + S-adenosyl-L-methionine
-
 Haemophilus parahaemolyticus DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.37 DNA (cytosine-C5) methyltransferase
-
 Haemophilus parahaemolyticus
2.1.1.37 M.HhaI
-
 Haemophilus parahaemolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.37 37
-
 assay at Haemophilus parahaemolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.37 7.5
-
 assay at Haemophilus parahaemolyticus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.37 S-adenosyl-L-methionine the ternary complexes between the enzyme, 2-(1H)-pyrimidinone inhibitor, and the cofactor S-adenosyl-L-methionine are maintained through the formation of a reversible covalent interaction Haemophilus parahaemolyticus