Any feedback?
Literature summary extracted from
- Inhibitory mechanisms of heterocyclic carboxaldehyde thiosemicabazones for two forms of human ribonucleotide reductase (2009), Biochem. Pharmacol., 78, 1178-1185.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.4.1 | ATP | - |
Homo sapiens |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.17.4.1 | expression of His6-tagged subunits M2, p53R2, and M1 in Escherichia coli strain BL21(DE3) | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.4.1 | 3,5-diaminopyridine-2-carboxaldehyde thiosemicarbazone | - |
Homo sapiens | |
| 1.17.4.1 | 3-amino-4-methylpyridine-2-carboxaldehyde thiosemicarbazone | - |
Homo sapiens | |
| 1.17.4.1 | 3-aminopyridine-2-carboxaldehyde thiosemicarbazone | triapine | Homo sapiens | |
| 1.17.4.1 | 3-methyl aminopyridine-2-carboxaldehyde thiosemicarbazone | - |
Homo sapiens | |
| 1.17.4.1 | 5-amino-4-morpholinomethylpyridine-2-carboxaldehyde thiosemicarbazone | - |
Homo sapiens | |
| 1.17.4.1 | 5-aminopyridine-2-carboxaldehyde thiosemicarbazone | - |
Homo sapiens | |
| 1.17.4.1 | 5-hydroxy-4-methyl-1-formylisoquinoline thiosemicarbazone | - |
Homo sapiens | |
| 1.17.4.1 | 5-methyl-4-amino-1-formylisoquinoline thiosemicarbazone | - |
Homo sapiens | |
| 1.17.4.1 | gemcitabine | - |
Homo sapiens | |
| 1.17.4.1 | Hydroxyurea | - |
Homo sapiens | |
| 1.17.4.1 | additional information | inhibitory mechanisms of heterocyclic carboxaldehyde thiosemicabazones | Homo sapiens |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.4.1 | Fe3+ | - |
Homo sapiens | |
| 1.17.4.1 | Mg2+ | - |
Homo sapiens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.17.4.1 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.17.4.1 | recombinant His6-tagged subunits M2, p53R2, and M1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.17.4.1 | KB cell | nasopharyngeal carcinoma cells, a gemcitabine-resistant cell line | Homo sapiens | - |
| 1.17.4.1 | nasopharyngeal carcinoma cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.4.1 | additional information | dCDP is produced from CDP by the holoenzyme | Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.17.4.1 | tetramer | the enzyme consists of M1, M2, and p53R2 subunits in an alphabeta2gamma constellation | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.17.4.1 | ribonucleotide reductase | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.4.1 | 37 | - |
catalytic activity and ligand binding assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.4.1 | 7.2 | - |
catalytic activity assay at | Homo sapiens |
| 1.17.4.1 | 7.5 | - |
ligand binding assay at | Homo sapiens |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.17.4.1 | physiological function | the enzyme is involved in DNA replication and damage repair, respectively | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
