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Literature summary extracted from

  • Lee, T.N.; Alborn, W.E.; Knierman, M.D.; Konrad, R.J.
    The diabetogenic antibiotic streptozotocin modifies the tryptic digest pattern for peptides of the enzyme O-GlcNAc-selective N-acetyl-beta-D-glucosaminidase that contain amino acid residues essential for enzymatic activity (2006), Biochem. Pharmacol., 72, 710-718.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.169 expressed in Escherichia coli BL21 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.169 C896W site-specific mutagenesis, in the tryptic peptide LGCFEIAK (894-901). Site-specific mutagenesis of the C-terminal glycine and cysteine residues (G895A and C896W) present in the tryptic peptide LGCFEIAK (894–901) causes little or no inhibition of O-GlcNAcase activity (72.3% of control and 129.9% of control, respectively) Homo sapiens
3.2.1.169 E130A site-specific mutagenesis, in the tryptic peptide EYEIEFIYAISPGLDITFSNPK (128-149), retains almost full enzymatic activity (97.3% of control) Homo sapiens
3.2.1.169 F133V site-specific mutagenesis, in the tryptic peptide EYEIEFIYAISPGLDITFSNPK (128-149), no O-GlcNAcase activity (3.3% of control) Homo sapiens
3.2.1.169 G895A site-specific mutagenesis, in the tryptic peptide LGCFEIAK (894-901). Site-specific mutagenesis of the C-terminal glycine and cysteine residues (G895A and C896W) present in the tryptic peptide LGCFEIAK (894–901) causes little or no inhibition of O-GlcNAcase activity (72.3% of control and 129.9% of control, respectively) Homo sapiens
3.2.1.169 S138I site-specific mutagenesis, in the tryptic peptide EYEIEFIYAISPGLDITFSNPK (128-149), exhibits greatly reduced activity (15.4% of control) Homo sapiens
3.2.1.169 Y129F site-specific mutagenesis, in the tryptic peptide EYEIEFIYAISPGLDITFSNPK (128-149), retains almost full enzymatic activity (90.5% of control) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.169 additional information methylnitrosourea (MNU), the nitric oxide-containing portion of streptozotocin: no significant inhibition observed Homo sapiens
3.2.1.169 O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate PUGNAc Homo sapiens
3.2.1.169 streptozotocin STZ, significantly dose-dependently inhibited recombinant O-GlcNAcase Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.169 130000
-
 recombinant O-GlcNAcase, Coomassie blue staining following one-dimensional gel electrophoresis and Western blotting Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.169 additional information Homo sapiens enzyme removes N-acetyl-beta-D-glucosaminide from proteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.169 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.169
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.169 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Homo sapiens 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.169 additional information enzyme removes N-acetyl-beta-D-glucosaminide from proteins Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.169 O-GlcNAc-selective N-acetyl-beta-D-glucosaminidase
-
 Homo sapiens
3.2.1.169 O-GlcNAcase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.169 7
-
 assay at Homo sapiens