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Literature summary extracted from

  • Denton, H.; Fyffe, S.; Smith, T.K.
    GDP-Mannose pyrophosphorylase is essential in the bloodstream form of Trypanosoma brucei (2010), Biochem. J., 425, 603-614.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.13 His tagged version expressed in Escherichia coli BL21 Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.13 GDP-6-deoyxmannose
-
 Trypanosoma brucei
2.7.7.13 GDP-mannose feedback inhibition Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.13 0.012
-
 D-mannose 1-phosphate
-
 Trypanosoma brucei
2.7.7.13 0.067
-
 GTP
-
 Trypanosoma brucei
2.7.7.13 0.29
-
 ATP
-
 Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.13 cytosol in bloodstream-form Trypanosoma brucei 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.13 Mg2+ absolute requirement for divalent cations, partially replaced by Mn2+ and Cu2+ Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.13 42000
-
 2 * 42000, SDS-PAGE, 2 * 41769.10, ESI-MS (41769.32 theoretical mass) Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.13 Trypanosoma brucei B9WNA1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.13 batch absorption to nickel-agarose Trypanosoma brucei

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.13 0.34
-
-
 Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.13 ATP + D-mannose 1-phosphate
-
 Trypanosoma brucei diphosphate + ADP-mannose
-
 ?
2.7.7.13 GTP + D-mannose 1-phosphate
-
 Trypanosoma brucei diphosphate + GDP-mannose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.13 homodimer 2 * 42000, SDS-PAGE, 2 * 41769.10, ESI-MS (41769.32 theoretical mass) Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
2.7.7.13 guanidine diphosphomannose pyrophosphorylase
-
 Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.13 additional information
-
 neutral pH optimum Trypanosoma brucei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.13 0.00125
-
 GDP-mannose competitive inhibitor with respect to GTP Trypanosoma brucei
2.7.7.13 0.002
-
 GDP-mannose mixed-type inhibition with respect to mannose 1-phosphate Trypanosoma brucei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.7.13 additional information
-
 IC50 values for ATP and CTP above 0.625 mM Trypanosoma brucei additional information
2.7.7.13 0.008
-
 feedback inhibition Trypanosoma brucei GDP-mannose
2.7.7.13 0.013
-
-
 Trypanosoma brucei GDP-6-deoxymannose

General Information

EC Number General Information Comment Organism
2.7.7.13 physiological function essential in bloodstream-form Trypanosoma brucei