BRENDA - Enzyme Database

Characterization of the heme oxygenase protein family in Arabidopsis thaliana reveals a diversity of functions

Gisk, B.; Yasui, Y.; Kohchi, T.; Frankenberg-Dinkel, N.; Biochem. J. 425, 425-434 (2010)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.14.18
ascorbate
addition of a second electron donor like ascorbate leads to 10times faster heme conversion
Arabidopsis thaliana
1.14.14.18
additional information
heme turnover is promoted by light when spinach thylakoids are present
Arabidopsis thaliana
1.14.15.20
L-ascorbate
adding of a second electron donor, such as ascorbate, led to a 10fold increase in the heme conversion rate
Arabidopsis thaliana
Cloned(Commentary)
EC Number
Commentary
Organism
1.14.14.18
HY1, HO3, and HO4 expressed without their predicted chloroplast transit peptides either using a tac promoter-driven N-terminal GST fusion expression system (HY1) or the pET vector system (HO3, HO4) using a T7-promoter and a His-tag fusion. Plasmids pGEX-mHY1, pET24a-mHO3 and pET24a-mHO4 expressed in Escherichia coli BL21 (DE3). Fluorescent protein expression in Nicotiana benthamiana by using Agrobacterium tumefaciens C58C1 pGV2260
Arabidopsis thaliana
1.14.15.20
expressed in Nicotiana benthamiana and Escherichia coli BL21 (DE3) cells
Arabidopsis thaliana
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.18
0.0027
-
heme
HO3, without ascorbate
Arabidopsis thaliana
1.14.14.18
0.0057
-
heme
HO4, without ascorbate
Arabidopsis thaliana
1.14.15.20
0.0013
-
protoheme
isoform HY1, at pH 7.2 and 25°C
Arabidopsis thaliana
1.14.15.20
0.0027
-
protoheme
isoform HO3, at pH 7.2 and 25°C
Arabidopsis thaliana
1.14.15.20
0.0057
-
protoheme
isoform HO4, at pH 7.2 and 25°C
Arabidopsis thaliana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.18
chloroplast
HY1, HO3, and HO4 are present as the processed mature protein in the plastid compartment
Arabidopsis thaliana
9507
-
1.14.14.18
additional information
no mitochondrial localization of HY1, HO3, and HO4
Arabidopsis thaliana
-
-
1.14.15.20
chloroplast
-
Arabidopsis thaliana
9507
-
1.14.15.20
nucleus
-
Arabidopsis thaliana
5634
-
1.14.15.20
plastid
isoforms HY1, HO3 and HO4
Arabidopsis thaliana
9536
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.14.18
27000
-
1 * 27000, HY1, SDS-PAGE. HY1, HO3 and HO4, gel filtration
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Arabidopsis thaliana
-
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.18
Arabidopsis thaliana
-
-
-
1.14.15.20
Arabidopsis thaliana
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.14.18
HY1, HO3, and HO4. HO3 and HO4 purified by cobalt affinity chromatography, to over 90% purity
Arabidopsis thaliana
1.14.15.20
Talon-cobalt-affinity column chromatography
Arabidopsis thaliana
Storage Stability
EC Number
Storage Stability
Organism
1.14.14.18
0°C, 100 mM potassium phosphate buffer, pH 7.2, 4 days
Arabidopsis thaliana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.18
heme + ferredoxin + O2
HY1, HO3, and HO4
702147
Arabidopsis thaliana
biliverdin IXalpha + Fe2+ + CO + A + H2O
-
-
-
?
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
702147
Arabidopsis thaliana
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.14.18
monomer
1 * 27000, HY1, SDS-PAGE. HY1, HO3 and HO4, gel filtration
Arabidopsis thaliana
1.14.15.20
monomer
-
Arabidopsis thaliana
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.14.14.18
10
45
-
Arabidopsis thaliana
1.14.15.20
10
45
the relative enzyme activity increases between 10°C and 45°C
Arabidopsis thaliana
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.14.18
7
-
HO3
Arabidopsis thaliana
1.14.14.18
7.25
-
HY1 and HO4
Arabidopsis thaliana
1.14.15.20
7
-
isoform HO3
Arabidopsis thaliana
1.14.15.20
7.3
-
isoforms HO4 and HY1
Arabidopsis thaliana
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.18
heme
-
Arabidopsis thaliana
1.14.14.18
NADPH
-
Arabidopsis thaliana
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.14.18
ascorbate
addition of a second electron donor like ascorbate leads to 10times faster heme conversion
Arabidopsis thaliana
1.14.14.18
additional information
heme turnover is promoted by light when spinach thylakoids are present
Arabidopsis thaliana
1.14.15.20
L-ascorbate
adding of a second electron donor, such as ascorbate, led to a 10fold increase in the heme conversion rate
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.18
HY1, HO3, and HO4 expressed without their predicted chloroplast transit peptides either using a tac promoter-driven N-terminal GST fusion expression system (HY1) or the pET vector system (HO3, HO4) using a T7-promoter and a His-tag fusion. Plasmids pGEX-mHY1, pET24a-mHO3 and pET24a-mHO4 expressed in Escherichia coli BL21 (DE3). Fluorescent protein expression in Nicotiana benthamiana by using Agrobacterium tumefaciens C58C1 pGV2260
Arabidopsis thaliana
1.14.15.20
expressed in Nicotiana benthamiana and Escherichia coli BL21 (DE3) cells
Arabidopsis thaliana
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.18
heme
-
Arabidopsis thaliana
1.14.14.18
NADPH
-
Arabidopsis thaliana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.18
0.0027
-
heme
HO3, without ascorbate
Arabidopsis thaliana
1.14.14.18
0.0057
-
heme
HO4, without ascorbate
Arabidopsis thaliana
1.14.15.20
0.0013
-
protoheme
isoform HY1, at pH 7.2 and 25°C
Arabidopsis thaliana
1.14.15.20
0.0027
-
protoheme
isoform HO3, at pH 7.2 and 25°C
Arabidopsis thaliana
1.14.15.20
0.0057
-
protoheme
isoform HO4, at pH 7.2 and 25°C
Arabidopsis thaliana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.18
chloroplast
HY1, HO3, and HO4 are present as the processed mature protein in the plastid compartment
Arabidopsis thaliana
9507
-
1.14.14.18
additional information
no mitochondrial localization of HY1, HO3, and HO4
Arabidopsis thaliana
-
-
1.14.15.20
chloroplast
-
Arabidopsis thaliana
9507
-
1.14.15.20
nucleus
-
Arabidopsis thaliana
5634
-
1.14.15.20
plastid
isoforms HY1, HO3 and HO4
Arabidopsis thaliana
9536
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.14.18
27000
-
1 * 27000, HY1, SDS-PAGE. HY1, HO3 and HO4, gel filtration
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Arabidopsis thaliana
-
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.18
HY1, HO3, and HO4. HO3 and HO4 purified by cobalt affinity chromatography, to over 90% purity
Arabidopsis thaliana
1.14.15.20
Talon-cobalt-affinity column chromatography
Arabidopsis thaliana
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.14.14.18
0°C, 100 mM potassium phosphate buffer, pH 7.2, 4 days
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.18
heme + ferredoxin + O2
HY1, HO3, and HO4
702147
Arabidopsis thaliana
biliverdin IXalpha + Fe2+ + CO + A + H2O
-
-
-
?
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
702147
Arabidopsis thaliana
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.14.18
monomer
1 * 27000, HY1, SDS-PAGE. HY1, HO3 and HO4, gel filtration
Arabidopsis thaliana
1.14.15.20
monomer
-
Arabidopsis thaliana
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.14.14.18
10
45
-
Arabidopsis thaliana
1.14.15.20
10
45
the relative enzyme activity increases between 10°C and 45°C
Arabidopsis thaliana
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.14.18
7
-
HO3
Arabidopsis thaliana
1.14.14.18
7.25
-
HY1 and HO4
Arabidopsis thaliana
1.14.15.20
7
-
isoform HO3
Arabidopsis thaliana
1.14.15.20
7.3
-
isoforms HO4 and HY1
Arabidopsis thaliana