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Literature summary extracted from

  • Paradis-Bleau, C.; Lloyd, A.; Sanschagrin, F.; Maaroufi, H.; Clarke, T.; Blewett, A.; Dowson, C.; Roper, D.I.; Bugg, T.D.; Levesque, R.C.
    Pseudomonas aeruginosa MurE amide ligase: enzyme kinetics and peptide inhibitor (2009), Biochem. J., 421, 263-272.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.13 MurEP1 time-dependent inhibition Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.13 0.03
-
 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
 Pseudomonas aeruginosa
6.3.2.13 0.14
-
 meso-diaminopimelic acid
-
 Pseudomonas aeruginosa
6.3.2.13 0.225
-
 ATP
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.13 55000
-
 SDS-PAGE Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.13 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.13
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.13 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + ATP + meso-diaminopimelic acid assay at pH 8.0 Pseudomonas aeruginosa ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.13 amide ligase
-
 Pseudomonas aeruginosa
6.3.2.13 MurE
-
 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.13 8
-
 assay at Pseudomonas aeruginosa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.3.2.13 0.5
-
 30 min pre-incupation Pseudomonas aeruginosa MurEP1
6.3.2.13 0.8
-
 10 min pre-incubation Pseudomonas aeruginosa MurEP1