EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.38 | expressed in HeLa cells and as a fusion protein with maltose-binding protein in Escherichia coli BL21(DE3) cells | Homo sapiens |
2.4.1.133 | cloning and expression of human membrane beta1,4-GalT7 in HeLa cells, expression of soluble form as a fusion protein with maltose-binding protein in Escherichia coli | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.38 | additional information | not inhibited by CDP, UDP-mannose, and UDP-N-acetyl-beta-D-glucosamine | Homo sapiens | |
2.4.1.38 | TDP | - |
Homo sapiens | |
2.4.1.38 | UDP | - |
Homo sapiens | |
2.4.1.38 | UTP | - |
Homo sapiens | |
2.4.1.133 | CDP | - |
Homo sapiens | |
2.4.1.133 | TDP | - |
Homo sapiens | |
2.4.1.133 | UDP | - |
Homo sapiens | |
2.4.1.133 | UDP-mannose | - |
Homo sapiens | |
2.4.1.133 | UDP-N-acetylgalactosamine | - |
Homo sapiens | |
2.4.1.133 | UTP | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.38 | 0.16 | - |
4-methylumbelliferyl beta-D-xylopyranoside | purified beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 0.16 | - |
UDP-xylose | purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 0.23 | - |
UDP-galactose | purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 0.27 | - |
4-methylumbelliferyl beta-D-xylopyranoside | purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 0.28 | - |
UDP-glucose | purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 0.31 | - |
UDP-galactose | purified beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 0.85 | - |
4-nitrophenyl beta-D-xylopyranoside | purified beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 1.27 | - |
4-nitrophenyl beta-D-xylopyranoside | purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 4 | - |
UDP-glucuronic acid | Km above 4 mM, purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 10 | - |
UDP-N-acetyl-alpha-D-glucosamine | Km above 10 mM, purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.133 | additional information | - |
UDP-N-acetyl glucosamine | KM value is above 10 in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
2.4.1.133 | additional information | - |
UDP-glucuronic acid | KM value is above 4 in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
2.4.1.133 | additional information | - |
UDP-N-acetyl galactosamine | KM value is not detected in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
2.4.1.133 | additional information | - |
UDP-mannose | KM value is not detected, in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
2.4.1.133 | 0.13 | - |
UDP-xylose | in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
2.4.1.133 | 0.16 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | membrane beta1,4-GalT7 in HeLa cells | Homo sapiens | |
2.4.1.133 | 0.23 | - |
UDP-galactose | in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
2.4.1.133 | 0.27 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | maltose-binding protein-beta1,4-GalT7 fusion protein | Homo sapiens | |
2.4.1.133 | 0.28 | - |
UDP-glucose | in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
2.4.1.133 | 0.31 | - |
UDP-galactose | membrane beta1,4-GalT7 in HeLa cells | Homo sapiens | |
2.4.1.133 | 0.85 | - |
4-nitrophenyl-beta-D-xylopyranoside | membrane beta1,4-GalT7 in HeLa cells | Homo sapiens | |
2.4.1.133 | 1.27 | - |
4-nitrophenyl-beta-D-xylopyranoside | maltose-binding protein-beta1,4-GalT7 fusion protein | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.38 | soluble | - |
Homo sapiens | - |
- |
2.4.1.133 | Golgi membrane | - |
Homo sapiens | 139 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.133 | Mn2+ | enzyme catalyses the Mn2+-dependent transfer of galactose from UDP-galactose the non-reducing end of the xylose residue of the common tetrasaccharide linker region of glycosaminoglycans | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.38 | 70000 | - |
purified maltose-binding protein-beta-1,4-GalT7 protein, SDS/PAGE | Homo sapiens |
2.4.1.133 | 70000 | - |
recombinant fusion protein, by SDS-PAGE | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.133 | UDP-galactose + O-beta-D-xylosylprotein | Homo sapiens | - |
UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.38 | Homo sapiens | - |
- |
- |
2.4.1.133 | Homo sapiens | Q9UBV7 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.38 | amylose column chromatography | Homo sapiens |
2.4.1.133 | purification of maltose-binding protein-beta1,4-GalT7 fusion protein with an amylose column | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.133 | 0.058 | - |
maltose-binding protein-fusion enzyme, supernatant, in 100 mM cacodylate buffer containing 10 mM MnCl2, pH 7.0 | Homo sapiens |
2.4.1.133 | 0.97 | - |
maltose-binding protein-fusion enzyme, amylose elution, in 100 mM cacodylate buffer containing 10 mM MnCl2, pH 7.0 | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.38 | additional information | UDP-mannose and UDP-N-acetyl galactosamine are no substrates for soluble beta1,4-GalT7 | Homo sapiens | ? | - |
? | |
2.4.1.38 | UDP-galactose + 4-methylumbelliferyl beta-D-xylopyranoside | - |
Homo sapiens | UDP + ? | - |
? | |
2.4.1.38 | UDP-galactose + 4-nitrophenyl beta-D-xylopyranoside | - |
Homo sapiens | UDP + beta-D-galactopyranosyl-1,4-xylopyranosyl-1-O-4-nitrophenol | - |
? | |
2.4.1.38 | UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide | 100% activity with UDP-galactose | Homo sapiens | UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide | - |
? | |
2.4.1.38 | UDP-glucose + 4-methylumbelliferyl beta-D-xylopyranoside | 1.5% activity with UDO-glucose compared to UDP-galactose | Homo sapiens | UDP + 4-O-beta-D-galactosyl-beta-D-glucose | - |
? | |
2.4.1.38 | UDP-glucuronic acid + 4-methylumbelliferyl beta-D-xylopyranoside | - |
Homo sapiens | UDP + ? | - |
? | |
2.4.1.38 | UDP-N-acetyl-alpha-D-glucosamine + 4-methylumbelliferyl beta-D-xylopyranoside | - |
Homo sapiens | UDP + ? | - |
? | |
2.4.1.38 | UDP-N-acetyl-alpha-D-glucosamine + 4-methylumbelliferyl beta-D-xylopyranoside | - |
Homo sapiens | ? | - |
? | |
2.4.1.38 | UDP-xylose + 4-methylumbelliferyl beta-D-xylopyranoside | - |
Homo sapiens | UDP + ? | - |
? | |
2.4.1.133 | additional information | enzyme is able to transfer glucose, xylose and, to a lesser extent glucuronic acid and N-acetylglucosamine, to acceptor sugars, whereas UDP-mannose and UDP-N-acetyl galactosamine are not substrates | Homo sapiens | ? | - |
? | |
2.4.1.133 | UDP-galactose + O-beta-D-xylosylprotein | - |
Homo sapiens | UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein | - |
? | |
2.4.1.133 | UDP-glucose + 4-methylumbelliferyl-beta-D-xylopyranoside | - |
Homo sapiens | ? | - |
? | |
2.4.1.133 | UDP-glucuronic acid + 4-methylumbelliferyl-beta-D-xylopyranoside | - |
Homo sapiens | ? | - |
? | |
2.4.1.133 | UDP-N-acetyl glucosamine + 4-methylumbelliferyl-beta-D-xylopyranoside | - |
Homo sapiens | ? | - |
? | |
2.4.1.133 | UDP-xylose + 4-methylumbelliferyl-beta-D-xylopyranoside | - |
Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.38 | beta1,4-galactosyltransferase 7 | - |
Homo sapiens |
2.4.1.38 | beta1,4-GalT7 | - |
Homo sapiens |
2.4.1.38 | galactosyltransferase 7 | - |
Homo sapiens |
2.4.1.133 | beta1,4-galactosyltransferase 7 | - |
Homo sapiens |
2.4.1.133 | beta1,4-GalT | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.133 | 37 | - |
assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.38 | 0.003 | - |
UDP-glucuronic acid | Km above 0.003 mM, purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 0.003 | - |
UDP-N-acetyl-alpha-D-glucosamine | Km above 0.003 mM, purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 0.01 | - |
UDP-xylose | purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 0.02 | - |
UDP-glucose | purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 1.13 | - |
4-methylumbelliferyl beta-D-xylopyranoside | purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 1.14 | - |
UDP-galactose | Km above 0.003 mM, purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 1.52 | - |
4-nitrophenyl beta-D-xylopyranoside | purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.133 | additional information | - |
UDP-glucuronic acid | kcat value is above 0.2 1/min in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
2.4.1.133 | additional information | - |
UDP-N-acetyl glucosamine | kcat value is above 0.2 1/min in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
2.4.1.133 | additional information | - |
UDP-mannose | kcat value is not detected, in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
2.4.1.133 | additional information | - |
UDP-N-acetyl galactosamine | kcat value not detected in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
2.4.1.133 | 3 | 6 | UDP-xylose | in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
2.4.1.133 | 72 | - |
UDP-glucose | in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
2.4.1.133 | 4080 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | recombinant maltose-binding protein-beta1,4-GalT7 fusion protein | Homo sapiens | |
2.4.1.133 | 5086 | - |
UDP-galactose | in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
2.4.1.133 | 5460 | - |
4-nitrophenyl-beta-D-xylopyranoside | recombinant maltose-binding protein-beta1,4-GalT7 fusion protein | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.133 | 7 | - |
assay at | Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.38 | 0.61 | - |
UDP | purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 1.65 | - |
UTP | purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 1.93 | - |
TDP | purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.133 | additional information | - |
CDP | Ki value is above 9 mM, UDP-galactose 0.1-16 mM, 0-10 mM CDP | Homo sapiens | |
2.4.1.133 | additional information | - |
UDP-N-acetyl galactosamine | Ki value is above 9 mM, UDP-galactose 0.1-16 mM, 0-10 mM UDP-N-acetyl galactosamine | Homo sapiens | |
2.4.1.133 | additional information | - |
UDP-mannose | Ki value is above 9, UDP-galactose 0.1-16 mM, 0-10 mM UDP-mannose | Homo sapiens | |
2.4.1.133 | 0.61 | - |
UDP | UDP-galactose 0.1-16 mM, UDP 0-2 mM | Homo sapiens | |
2.4.1.133 | 1.65 | - |
UTP | UDP-galactose 0.1-16 mM, UTP 0-4 mM | Homo sapiens | |
2.4.1.133 | 1.93 | - |
TDP | UDP-galactose 0.1-16 mM, TDP 0-4 mM | Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.1.133 | physiological function | enzyme is involved in the biosynthesis of the tetrasaccharide linker protein region (glucuronic acid beta1,3galactose beta1,3galactose beta1,4xylose beta1) of proteoglycans, by catalysing the transfer of galactose from the uridine 5-diphosphogalactose to a xylose residue, this reaction is rate-limiting in glycosaminoglycan biosynthesis | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.38 | 0.065 | - |
UDP-xylose | purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 0.07 | - |
UDP-glucose | purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.4.1.38 | 4.93 | - |
UDP-galactose | Km above 0.003 mM, purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens |