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Literature summary extracted from

  • Daligault, F.; Rahuel-Clermont, S.; Gulberti, S.; Cung, M.T.; Branlant, G.; Netter, P.; Magdalou, J.; Lattard, V.
    Thermodynamic insights into the structural basis governing the donor substrate recognition by human beta1,4-galactosyltransferase 7 (2009), Biochem. J., 418, 605-614.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.38 expressed in HeLa cells and as a fusion protein with maltose-binding protein in Escherichia coli BL21(DE3) cells Homo sapiens
2.4.1.133 cloning and expression of human membrane beta1,4-GalT7 in HeLa cells, expression of soluble form as a fusion protein with maltose-binding protein in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.38 additional information not inhibited by CDP, UDP-mannose, and UDP-N-acetyl-beta-D-glucosamine Homo sapiens
2.4.1.38 TDP
-
Homo sapiens
2.4.1.38 UDP
-
Homo sapiens
2.4.1.38 UTP
-
Homo sapiens
2.4.1.133 CDP
-
Homo sapiens
2.4.1.133 TDP
-
Homo sapiens
2.4.1.133 UDP
-
Homo sapiens
2.4.1.133 UDP-mannose
-
Homo sapiens
2.4.1.133 UDP-N-acetylgalactosamine
-
Homo sapiens
2.4.1.133 UTP
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.38 0.16
-
4-methylumbelliferyl beta-D-xylopyranoside purified beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 0.16
-
UDP-xylose purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 0.23
-
UDP-galactose purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 0.27
-
4-methylumbelliferyl beta-D-xylopyranoside purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 0.28
-
UDP-glucose purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 0.31
-
UDP-galactose purified beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 0.85
-
4-nitrophenyl beta-D-xylopyranoside purified beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 1.27
-
4-nitrophenyl beta-D-xylopyranoside purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 4
-
UDP-glucuronic acid Km above 4 mM, purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 10
-
UDP-N-acetyl-alpha-D-glucosamine Km above 10 mM, purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.133 additional information
-
UDP-N-acetyl glucosamine KM value is above 10 in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
2.4.1.133 additional information
-
UDP-glucuronic acid KM value is above 4 in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
2.4.1.133 additional information
-
UDP-N-acetyl galactosamine KM value is not detected in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
2.4.1.133 additional information
-
UDP-mannose KM value is not detected, in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
2.4.1.133 0.13
-
UDP-xylose in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
2.4.1.133 0.16
-
4-methylumbelliferyl-beta-D-xylopyranoside membrane beta1,4-GalT7 in HeLa cells Homo sapiens
2.4.1.133 0.23
-
UDP-galactose in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
2.4.1.133 0.27
-
4-methylumbelliferyl-beta-D-xylopyranoside maltose-binding protein-beta1,4-GalT7 fusion protein Homo sapiens
2.4.1.133 0.28
-
UDP-glucose in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
2.4.1.133 0.31
-
UDP-galactose membrane beta1,4-GalT7 in HeLa cells Homo sapiens
2.4.1.133 0.85
-
4-nitrophenyl-beta-D-xylopyranoside membrane beta1,4-GalT7 in HeLa cells Homo sapiens
2.4.1.133 1.27
-
4-nitrophenyl-beta-D-xylopyranoside maltose-binding protein-beta1,4-GalT7 fusion protein Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.38 soluble
-
Homo sapiens
-
-
2.4.1.133 Golgi membrane
-
Homo sapiens 139
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.133 Mn2+ enzyme catalyses the Mn2+-dependent transfer of galactose from UDP-galactose the non-reducing end of the xylose residue of the common tetrasaccharide linker region of glycosaminoglycans Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.38 70000
-
purified maltose-binding protein-beta-1,4-GalT7 protein, SDS/PAGE Homo sapiens
2.4.1.133 70000
-
recombinant fusion protein, by SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.133 UDP-galactose + O-beta-D-xylosylprotein Homo sapiens
-
UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.38 Homo sapiens
-
-
-
2.4.1.133 Homo sapiens Q9UBV7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.38 amylose column chromatography Homo sapiens
2.4.1.133 purification of maltose-binding protein-beta1,4-GalT7 fusion protein with an amylose column Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.133 0.058
-
maltose-binding protein-fusion enzyme, supernatant, in 100 mM cacodylate buffer containing 10 mM MnCl2, pH 7.0 Homo sapiens
2.4.1.133 0.97
-
maltose-binding protein-fusion enzyme, amylose elution, in 100 mM cacodylate buffer containing 10 mM MnCl2, pH 7.0 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.38 additional information UDP-mannose and UDP-N-acetyl galactosamine are no substrates for soluble beta1,4-GalT7 Homo sapiens ?
-
?
2.4.1.38 UDP-galactose + 4-methylumbelliferyl beta-D-xylopyranoside
-
Homo sapiens UDP + ?
-
?
2.4.1.38 UDP-galactose + 4-nitrophenyl beta-D-xylopyranoside
-
Homo sapiens UDP + beta-D-galactopyranosyl-1,4-xylopyranosyl-1-O-4-nitrophenol
-
?
2.4.1.38 UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide 100% activity with UDP-galactose Homo sapiens UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
?
2.4.1.38 UDP-glucose + 4-methylumbelliferyl beta-D-xylopyranoside 1.5% activity with UDO-glucose compared to UDP-galactose Homo sapiens UDP + 4-O-beta-D-galactosyl-beta-D-glucose
-
?
2.4.1.38 UDP-glucuronic acid + 4-methylumbelliferyl beta-D-xylopyranoside
-
Homo sapiens UDP + ?
-
?
2.4.1.38 UDP-N-acetyl-alpha-D-glucosamine + 4-methylumbelliferyl beta-D-xylopyranoside
-
Homo sapiens UDP + ?
-
?
2.4.1.38 UDP-N-acetyl-alpha-D-glucosamine + 4-methylumbelliferyl beta-D-xylopyranoside
-
Homo sapiens ?
-
?
2.4.1.38 UDP-xylose + 4-methylumbelliferyl beta-D-xylopyranoside
-
Homo sapiens UDP + ?
-
?
2.4.1.133 additional information enzyme is able to transfer glucose, xylose and, to a lesser extent glucuronic acid and N-acetylglucosamine, to acceptor sugars, whereas UDP-mannose and UDP-N-acetyl galactosamine are not substrates Homo sapiens ?
-
?
2.4.1.133 UDP-galactose + O-beta-D-xylosylprotein
-
Homo sapiens UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein
-
?
2.4.1.133 UDP-glucose + 4-methylumbelliferyl-beta-D-xylopyranoside
-
Homo sapiens ?
-
?
2.4.1.133 UDP-glucuronic acid + 4-methylumbelliferyl-beta-D-xylopyranoside
-
Homo sapiens ?
-
?
2.4.1.133 UDP-N-acetyl glucosamine + 4-methylumbelliferyl-beta-D-xylopyranoside
-
Homo sapiens ?
-
?
2.4.1.133 UDP-xylose + 4-methylumbelliferyl-beta-D-xylopyranoside
-
Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.38 beta1,4-galactosyltransferase 7
-
Homo sapiens
2.4.1.38 beta1,4-GalT7
-
Homo sapiens
2.4.1.38 galactosyltransferase 7
-
Homo sapiens
2.4.1.133 beta1,4-galactosyltransferase 7
-
Homo sapiens
2.4.1.133 beta1,4-GalT
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.133 37
-
assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.38 0.003
-
UDP-glucuronic acid Km above 0.003 mM, purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 0.003
-
UDP-N-acetyl-alpha-D-glucosamine Km above 0.003 mM, purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 0.01
-
UDP-xylose purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 0.02
-
UDP-glucose purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 1.13
-
4-methylumbelliferyl beta-D-xylopyranoside purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 1.14
-
UDP-galactose Km above 0.003 mM, purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 1.52
-
4-nitrophenyl beta-D-xylopyranoside purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.133 additional information
-
UDP-glucuronic acid kcat value is above 0.2 1/min in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
2.4.1.133 additional information
-
UDP-N-acetyl glucosamine kcat value is above 0.2 1/min in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
2.4.1.133 additional information
-
UDP-mannose kcat value is not detected, in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
2.4.1.133 additional information
-
UDP-N-acetyl galactosamine kcat value not detected in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
2.4.1.133 3 6 UDP-xylose in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
2.4.1.133 72
-
UDP-glucose in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
2.4.1.133 4080
-
4-methylumbelliferyl-beta-D-xylopyranoside recombinant maltose-binding protein-beta1,4-GalT7 fusion protein Homo sapiens
2.4.1.133 5086
-
UDP-galactose in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
2.4.1.133 5460
-
4-nitrophenyl-beta-D-xylopyranoside recombinant maltose-binding protein-beta1,4-GalT7 fusion protein Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.133 7
-
assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.38 0.61
-
UDP purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 1.65
-
UTP purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 1.93
-
TDP purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.133 additional information
-
CDP Ki value is above 9 mM, UDP-galactose 0.1-16 mM, 0-10 mM CDP Homo sapiens
2.4.1.133 additional information
-
UDP-N-acetyl galactosamine Ki value is above 9 mM, UDP-galactose 0.1-16 mM, 0-10 mM UDP-N-acetyl galactosamine Homo sapiens
2.4.1.133 additional information
-
UDP-mannose Ki value is above 9, UDP-galactose 0.1-16 mM, 0-10 mM UDP-mannose Homo sapiens
2.4.1.133 0.61
-
UDP UDP-galactose 0.1-16 mM, UDP 0-2 mM Homo sapiens
2.4.1.133 1.65
-
UTP UDP-galactose 0.1-16 mM, UTP 0-4 mM Homo sapiens
2.4.1.133 1.93
-
TDP UDP-galactose 0.1-16 mM, TDP 0-4 mM Homo sapiens

General Information

EC Number General Information Comment Organism
2.4.1.133 physiological function enzyme is involved in the biosynthesis of the tetrasaccharide linker protein region (glucuronic acid beta1,3galactose beta1,3galactose beta1,4xylose beta1) of proteoglycans, by catalysing the transfer of galactose from the uridine 5-diphosphogalactose to a xylose residue, this reaction is rate-limiting in glycosaminoglycan biosynthesis Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.38 0.065
-
UDP-xylose purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 0.07
-
UDP-glucose purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.4.1.38 4.93
-
UDP-galactose Km above 0.003 mM, purified maltose-binding protein-beta1,4-GalT7, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens