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Literature summary extracted from

  • Jois, P.; Madhu, N.; Rao, D.
    Role of histidine residues in EcoP15I DNA methyltransferase activity as probed by chemical modification and site-directed mutagenesis (2008), Biochem. J., 410, 543-553.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.72 expressed in Escherichia coli BL21(DE3)pLysS cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.72 H171A the mutant is almost as active as the wild type enzyme Escherichia coli
2.1.1.72 H335A the mutant is catalytically inactive and binds to DNA more tightly than the wild type enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.72 diethyl dicarbonate the inactivation of the enzyme by diethyl dicarbonate is specific for histidine residues, pre-incubating the methylase with DNA is able to protect the enzyme from diethyl dicarbonate inactivation, hydroxylamine is unable to reverse the effect caused by diethyl dicarbonate Escherichia coli
2.1.1.72 additional information imidazole (5 mM) and ethanol (0.02% v/v) do not show any effect on enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Escherichia coli EcoP15I MTase adds a methyl group to the second adenine in the recognition sequence 5'-CAGCAG-3' in the presence of S-adenosyl-L-methionine S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.1.1.72 Ethanol ethanol (0.02% v/v) does not show any effect on enzyme Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.72 heparin-Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine EcoP15I MTase adds a methyl group to the second adenine in the recognition sequence 5'-CAGCAG-3' in the presence of S-adenosyl-L-methionine Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + pUC19 DNA adenine
-
 Escherichia coli S-adenosyl-L-homocysteine + pUC19 DNA 6-methyladenine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 DNA adenine methyltransferase
-
 Escherichia coli
2.1.1.72 EcoP15I MTase
-
 Escherichia coli
2.1.1.72 M.EcoP15I
-
 Escherichia coli