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Literature summary extracted from

  • Schrover, J.M.; Frank, J.; van Wielink, J.E.; Duine, J.A.
    Quaternary structure of quinoprotein ethanol dehydrogenase from Pseudomonas aeruginosa and its reoxidation with a novel cytochrome c from this organism (1993), Biochem. J., 290 (Pt 1), 123-127.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.8 Ca2+ rather loosely bound, necessary for pyrroloquinoline quinone binding and for stability of enzyme Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.2.8 9000
-
 2 * 60000, alpha-subunit, + 2 * 9000, beta-subunit, SDS-PAGE Pseudomonas aeruginosa
1.1.2.8 60000
-
 2 * 60000, alpha-subunit, + 2 * 9000, beta-subunit, SDS-PAGE Pseudomonas aeruginosa
1.1.2.8 136000
-
 gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.8 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.8
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.2.8 40
-
 pH 9.0, 25°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.8 ethanol + N,N,N',N'-tetramethyl-p-phenylenediamine i.e. Wurster's Blue Pseudomonas aeruginosa ethanal + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.2.8 More enzyme interacts with a soluble cytochrome cEDH, the oxidized form being an excellent acceptor for the semiquinone form of EDH. This cytochrome is quite different from the cytochrome c551 operating in nitrate respiration Pseudomonas aeruginosa
1.1.2.8 tetramer 2 * 60000, alpha-subunit, + 2 * 9000, beta-subunit, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.1.2.8 EDH
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.8 pyrroloquinoline quinone i.e. 2,7,9-tricarboxy-1H-pyrrolo-[2,3-f]quinoline-4,5-dione Pseudomonas aeruginosa