EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.3.13 | into the vector pET-151 for expression in Escherichia coli BL21DE3 Star cells | Neisseria meningitidis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.3.13 | E365Term | mutant, truncated form of NmeIPMS, loss of the regulatory domain results in a loss of the ability to catalyse the adol reaction, although the enzyme is still able to slowly hydrolyse AcCoA | Neisseria meningitidis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.13 | (S)-alpha-hydroxyisovalerate | - |
Neisseria meningitidis | |
2.3.3.13 | 3,3-dibromopyruvate | - |
Neisseria meningitidis | |
2.3.3.13 | 3-Bromopyruvate | - |
Neisseria meningitidis | |
2.3.3.13 | leucine | - |
Neisseria meningitidis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.13 | 0.027 | - |
3-methyl-2-oxopentanoate | pH 7.5, 25°C | Neisseria meningitidis | |
2.3.3.13 | 0.3 | - |
3-Fluoropyruvate | pH 7.5, 25°C | Neisseria meningitidis | |
2.3.3.13 | 0.63 | - |
pyruvate | pH 7.5, 25°C | Neisseria meningitidis | |
2.3.3.13 | 3.3 | - |
alpha-Ketobutyrate | pH 7.5, 25°C | Neisseria meningitidis | |
2.3.3.13 | 5.4 | - |
alpha-ketoisovalerate | pH 7.5, 25°C | Neisseria meningitidis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.13 | Cd2+ | - |
Neisseria meningitidis | |
2.3.3.13 | Zn2+ | - |
Neisseria meningitidis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 39930 | - |
E365Term mutant, theoretical | Neisseria meningitidis |
2.3.3.13 | 40000 | - |
E365Term mutant, determined by SDS-PAGE | Neisseria meningitidis |
2.3.3.13 | 56000 | - |
wild-type enzyme, determined by SDS-PAGE | Neisseria meningitidis |
2.3.3.13 | 56030 | - |
wild-type enzyme, theoretical | Neisseria meningitidis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.13 | acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Neisseria meningitidis | - |
(2S)-2-isopropylmalate + CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.13 | Neisseria meningitidis | Q9JZG1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.3.13 | using Talon Superflow Metal Affinity resin, the His-tag is removed by TEV protease, anion exchange chromatography on a SourceQ column is applied | Neisseria meningitidis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.13 | 2-oxobutyrate + acetyl-CoA + H2O | - |
Neisseria meningitidis | ? | - |
? | |
2.3.3.13 | 3-fluoropyruvate + acetyl-CoA + H2O | - |
Neisseria meningitidis | ? | - |
? | |
2.3.3.13 | 3-methyl-2-oxopentanoate + acetyl-CoA + H2O | - |
Neisseria meningitidis | ? | - |
? | |
2.3.3.13 | acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Neisseria meningitidis | (2S)-2-isopropylmalate + CoA | - |
? | |
2.3.3.13 | alpha-ketoisovalerate + acetyl-CoA + H2O | - |
Neisseria meningitidis | alpha-isopropylmalate + CoA | - |
? | |
2.3.3.13 | pyruvate + acetyl-CoA + H2O | - |
Neisseria meningitidis | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.3.13 | homodimer | - |
Neisseria meningitidis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.3.13 | alpha-IPMS | - |
Neisseria meningitidis |
2.3.3.13 | Alpha-isopropylmalate synthase | - |
Neisseria meningitidis |
2.3.3.13 | NmeIPMS | - |
Neisseria meningitidis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 25 | - |
activity assay | Neisseria meningitidis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.13 | 0.035 | - |
alpha-ketoisovalerate | pH 7.5, 25°C | Neisseria meningitidis | |
2.3.3.13 | 0.28 | - |
3-methyl-2-oxopentanoate | pH 7.5, 25°C | Neisseria meningitidis | |
2.3.3.13 | 1.36 | - |
alpha-Ketobutyrate | pH 7.5, 25°C | Neisseria meningitidis | |
2.3.3.13 | 1.5 | - |
pyruvate | pH 7.5, 25°C | Neisseria meningitidis | |
2.3.3.13 | 105 | - |
3-Fluoropyruvate | pH 7.5, 25°C | Neisseria meningitidis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 7.5 | - |
activity assay | Neisseria meningitidis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.13 | 0.03 | - |
3,3-dibromopyruvate | pH 7.5, 25°C | Neisseria meningitidis | |
2.3.3.13 | 0.06 | - |
3-Bromopyruvate | pH 7.5, 25°C | Neisseria meningitidis | |
2.3.3.13 | 2.5 | - |
(S)-alpha-hydroxyisovalerate | pH 7.5, 25°C | Neisseria meningitidis |