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Literature summary extracted from
- Substrate recognition characteristics of human holocarboxylase synthetase for biotin ligation (2010), Biochem. Biophys. Res. Commun., 391, 455-460.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 6.3.4.15 | expression of HCS in Spodoptera frugiperda Sf9 cells and in Hi5 insect cells using the baculovirus transfection system | Homo sapiens |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.3.4.15 | Mg2+ | - |
Homo sapiens |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.3.4.15 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.3.4.15 | recombinant human HCS from Hi5 insect cells by nickel affinity chromatography, anion exchange chromatography, and gel filtration | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.3.4.15 | ATP + biotin + apo-[acetyl-CoA carboxylase 2] | i.e. ACC2, substrate is the recombinantly expressed His-tagged biotinoyl domain, ACC75, of ACC2, comprising residues 891-965. hHCS recognizes the MKM motif, NMR binding study, binding pattern, overview | Homo sapiens | AMP + diphosphate + acetyl-CoA carboxylase 2 | - |
? | |
| 6.3.4.15 | ATP + biotin + apo-[biotin carboxyl carrier protein] | i.e. BCCP, substrate is the recombinantly expressed His-tagged biotinoyl domain, BCCP87, of Escherichia coli BCCP, binding pattern, overview | Homo sapiens | AMP + diphosphate + biotin carboxyl carrier protein | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 6.3.4.15 | HCS | - |
Homo sapiens |
| 6.3.4.15 | Holocarboxylase synthetase | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 6.3.4.15 | 30 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.3.4.15 | 7.3 | - |
assay at | Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.3.4.15 | ATP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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