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Literature summary extracted from

  • Prasannan, P.; Suliman, H.S.; Robertus, J.D.
    Kinetic analysis of site-directed mutants of methionine synthase from Candida albicans (2009), Biochem. Biophys. Res. Commun., 382, 730-734.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.13 expressed as a GST fusion protein in Saccharomyces cerevisiae strain SDYalpha Candida albicans

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.13 D504A the mutant exhibits 4% of wild type activity Candida albicans
2.1.1.13 D504N the mutant exhibits 11% of wild type activity Candida albicans
2.1.1.13 D504S the mutant exhibits 7% of wild type activity Candida albicans
2.1.1.13 D614A the mutant exhibits less than 2% of wild type activity Candida albicans
2.1.1.13 D614N the mutant exhibits less than 2% of wild type activity Candida albicans
2.1.1.13 D614S the mutant exhibits 11% of wild type activity Candida albicans
2.1.1.13 E620Q the mutation has no effect on enzyme activity Candida albicans
2.1.1.13 E620S the mutation has no effect on enzyme activity Candida albicans
2.1.1.13 H128N the mutant is 5fold less active compared to the wild type GST fusion protein Candida albicans
2.1.1.13 R530A the mutant exhibits 33% of wild type activity Candida albicans
2.1.1.13 S448A the mutant exhibits 67% of wild type activity Candida albicans
2.1.1.13 W576F the mutant is 3fold less active compared to the wild type GST fusion protein Candida albicans
2.1.1.13 W576Y the mutant is 8fold less active compared to the wild type GST fusion protein Candida albicans
2.1.1.13 Y527F the mutant exhibits 67% of wild type activity Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.13 0.029
-
 L-homocysteine in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-HCl (pH 7.2), 0.1 mM MgSO4, and 10 mM dithiothreitol, at 37°C Candida albicans
2.1.1.13 0.165
-
 N5-methyltetrahydropteroylmonoglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-HCl (pH 7.2), 0.1 mM MgSO4, and 10 mM dithiothreitol, at 37°C Candida albicans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.13 86000
-
 SDS-PAGE Candida albicans
2.1.1.13 112000
-
 GST fusion protein, SDS-PAGE Candida albicans

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.13 Candida albicans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.13 glutathione agarose column chromatography Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Candida albicans tetrahydropteroylmonoglutamate + L-methionine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.13 Met6p
-
 Candida albicans