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Literature summary extracted from

  • Lotierzo, M.; Bui, B.T.; Leech, H.K.; Warren, M.J.; Marquet, A.; Rigby, S.E.
    Iron-sulfur cluster dynamics in biotin synthase: a new [2Fe-2S](1+) cluster (2009), Biochem. Biophys. Res. Commun., 381, 487-490.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.6 hexahistidine tagged proteins are expressed in Escherichia coli BL21DE3 pLysS cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.8.1.6 D155A mutation in the conserved YNHNLD sequence Escherichia coli
2.8.1.6 H152A mutation in the conserved YNHNLD sequence Escherichia coli
2.8.1.6 N151A mutation in the conserved YNHNLD sequence Escherichia coli
2.8.1.6 N153A mutation in the conserved YNHNLD sequence Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.6 dethiobiotin + sulfur + S-adenosyl-L-methionine Escherichia coli
-
 biotin + L-methionine + 5'-deoxyadenosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.6 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.6
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.6 dethiobiotin + sulfur + S-adenosyl-L-methionine
-
 Escherichia coli biotin + L-methionine + 5'-deoxyadenosine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.1.6 BioB
-
 Escherichia coli
2.8.1.6 biotin synthase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.1.6 iron-sulfur centre
-
 Escherichia coli
2.8.1.6 S-adenosyl-L-methionine
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.8.1.6 physiological function biotin synthase catalyses the final step in the biosynthesis of biotin Escherichia coli