BRENDA - Enzyme Database

Mammalian enzymes of trimethyllysine conversion to trimethylaminobutyrate

Henderson, L.M.; Nelson, P.J.; Henderson, L.; Fed. Proc. 41, 2843-2847 (1982)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.11.8
dithiothreitol
sparing as well as augmenting effect
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.11.8
alpha-N-Acetyltrimethyllysine
-
Bos taurus
1.14.11.8
citrate
-
Bos taurus
1.14.11.8
Hydroxytrimethyllysine
4 stereoisomers
Bos taurus
1.14.11.8
isocitrate
-
Bos taurus
1.14.11.8
Lactate
-
Bos taurus
1.14.11.8
malonate
-
Bos taurus
1.14.11.8
p-chloromercuribenzoate
-
Bos taurus
1.14.11.8
succinate
-
Bos taurus
1.14.11.8
Zn2+
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.11.8
0.13
-
N6,N6,N6-Trimethyl-L-lysine
-
Bos taurus
1.14.11.8
0.22
-
alpha-ketoglutarate
-
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.11.8
mitochondrion
-
Bos taurus
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.11.8
Ca2+
no stimulation
Bos taurus
1.14.11.8
Fe2+
preincubation with Fe2+ in absence of other cofactors inactivates (ascorbate or dithiothreitol prevents inactivation); required
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.11.8
52000
-
x * 52000 + x * 65000, SDS-PAGE
Bos taurus
1.14.11.8
65000
-
x * 52000 + x * 65000, SDS-PAGE
Bos taurus
1.14.11.8
180000
-
gel filtration
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.11.8
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.11.8
partial
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.8
kidney
cortex (67%), medulla (33%)
Bos taurus
-
1.14.11.8
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.14.11.8
additional information
-
-
Bos taurus
Storage Stability
EC Number
Storage Stability
Organism
1.14.11.8
0C, half-life: 5 days
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.11.8
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2
N6,N6, N6-trimethyl-L-lysine is epsilon-N-trimethyl-L-lysine
7020
Bos taurus
3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.14.11.8
?
x * 52000 + x * 65000, SDS-PAGE
Bos taurus
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.14.11.8
37
-
assay at
Bos taurus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.14.11.8
-
-
half-life: 5 days
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.11.8
6.8
-
assay at
Bos taurus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.11.8
ascorbate
required
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.11.8
dithiothreitol
sparing as well as augmenting effect
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.11.8
ascorbate
required
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.11.8
alpha-N-Acetyltrimethyllysine
-
Bos taurus
1.14.11.8
citrate
-
Bos taurus
1.14.11.8
Hydroxytrimethyllysine
4 stereoisomers
Bos taurus
1.14.11.8
isocitrate
-
Bos taurus
1.14.11.8
Lactate
-
Bos taurus
1.14.11.8
malonate
-
Bos taurus
1.14.11.8
p-chloromercuribenzoate
-
Bos taurus
1.14.11.8
succinate
-
Bos taurus
1.14.11.8
Zn2+
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.11.8
0.13
-
N6,N6,N6-Trimethyl-L-lysine
-
Bos taurus
1.14.11.8
0.22
-
alpha-ketoglutarate
-
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.11.8
mitochondrion
-
Bos taurus
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.11.8
Ca2+
no stimulation
Bos taurus
1.14.11.8
Fe2+
preincubation with Fe2+ in absence of other cofactors inactivates (ascorbate or dithiothreitol prevents inactivation); required
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.11.8
52000
-
x * 52000 + x * 65000, SDS-PAGE
Bos taurus
1.14.11.8
65000
-
x * 52000 + x * 65000, SDS-PAGE
Bos taurus
1.14.11.8
180000
-
gel filtration
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.11.8
partial
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.8
kidney
cortex (67%), medulla (33%)
Bos taurus
-
1.14.11.8
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.14.11.8
additional information
-
-
Bos taurus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.14.11.8
0C, half-life: 5 days
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.11.8
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2
N6,N6, N6-trimethyl-L-lysine is epsilon-N-trimethyl-L-lysine
7020
Bos taurus
3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.11.8
?
x * 52000 + x * 65000, SDS-PAGE
Bos taurus
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.14.11.8
37
-
assay at
Bos taurus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.14.11.8
-
-
half-life: 5 days
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.11.8
6.8
-
assay at
Bos taurus