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Literature summary extracted from

  • Mazzaferro, L.; Pinuel, L.; Minig, M.; Breccia, J.D.
    Extracellular monoenzyme deglycosylation system of 7-O-linked flavonoid beta-rutinosides and its disaccharide transglycosylation activity from Stilbella fimetaria (2010), Arch. Microbiol., 192, 383-393.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.168 1.77
-
hesperidin pH 5.0, 60°C Acremonium sp.
3.2.1.168 8.73
-
hesperidin methylchalcone pH 5.0, 60°C Acremonium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.168 cell bound
-
Acremonium sp.
-
-
3.2.1.168 extracellular
-
Acremonium sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.168 Ba2+ reduces the activity in the range of 10-50% Acremonium sp.
3.2.1.168 Ca2+ reduces the activity in the range of 10-50% Acremonium sp.
3.2.1.168 Cu2+ deleterious for enzyme activity Acremonium sp.
3.2.1.168 EDTA reduces the activity in the range of 10-50% Acremonium sp.
3.2.1.168 Fe2+ reduces the activity in the range of 10-50% Acremonium sp.
3.2.1.168 Hg2+ deleterious for enzyme activity Acremonium sp.
3.2.1.168 Mg2+ reduces the activity in the range of 10-50% Acremonium sp.
3.2.1.168 additional information no influence of manganese on the enzyme activity Acremonium sp.
3.2.1.168 Ni2+ reduces the activity in the range of 10-50% Acremonium sp.
3.2.1.168 Zn2+ reduces the activity in the range of 10-50% Acremonium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.168 42220
-
mass spectrometry Acremonium sp.
3.2.1.168 46000
-
SDS-PAGE Acremonium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.168 hesperidin + H2O Acremonium sp. pH 5.0, 60°C hesperetin + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.168 Ethanol increasing ethanol concentration 0-20% shows to diminish hesperidin hydrolysis, but total activity, hydrolysis plus transglycosylation activity increases Acremonium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.168 Acremonium sp.
-
SES201, isolated from South Patagonia, Argentina
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.168 glycoprotein
-
Acremonium sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.168 to homogeneity, fractionation with ammonium sulfate and two chromatographic steps: butyl-agarose and QAE-Sephadex Acremonium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.168 diosmin + H2O a 7-O-beta-rutinoside, pH 5.0, 60°C, 3.3% relative activity compared to hesperidin as substrate Acremonium sp. didymin + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
?
3.2.1.168 eriocitrin + H2O a 7-O-beta-rutinoside, pH 5.0, 60°C, 89.7% relative activity compared to hesperidin as substrate Acremonium sp. eriodictyol + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
?
3.2.1.168 hesperidin + H2O pH 5.0, 60°C Acremonium sp. hesperetin + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
?
3.2.1.168 hesperidin + H2O i.e. hesperitin 7-(6-O-alpha-rhamnopyranosyl)-beta-D-glucopyranoside, pH 5.0, 60°C, substrates and products of the enzymatic reaction are analyzed by UV-MALDI-TOF/TOF mass spectrometry Acremonium sp. hesperetin + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside i.e. hesperetin + rutinose, substrates and products of the enzymatic reaction are analyzed by UV-Maldi-TOF/TOF mass spectrometry ?
3.2.1.168 hesperidin methylchalcone + H2O a 7-O-beta-rutinoside, pH 5.0, 60°C, 58.5% relative activity compared to hesperidin as substrate Acremonium sp. hesperetin methylchalcone + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
?
3.2.1.168 additional information in addition, when incubated with 5% (v/v) ethanol as acceptor and hesperidin and hesperidin methylchalcone as sugar donors another glycosylated product is found as a result of a transglycosylation reaction: ethyl rutinoside, indicating that alpha-rhamnosyl-beta-glucosidase is able to transfer disaccharide units to OH-acceptors in an aqueous medium Acremonium sp. ?
-
?
3.2.1.168 additional information no dectectable enzyme activity using the 7-O-beta-neohesperidosides: naringin and neohesperidin as substrates, or the 3-O-beta-rutinoside rutin Acremonium sp. ?
-
?
3.2.1.168 narirutin + H2O a 7-O-beta-rutinoside, pH 5.0, 60°C, 61% relative activity compared to hesperidin as substrate Acremonium sp. naringenin + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.168 70
-
also active at 5°C, Tm of 74.6°C and a calculated activation energy Ea of 183.3 kJ/mol Acremonium sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.168 40 80 40°C: ca. 20% relative activity, 80°C: ca. 50% relative activity Acremonium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.168 74.6
-
Tm of 74.6°C and a calculated activation energy Ea of 183.3 kJ/mol Acremonium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.168 32.4
-
hesperidin pH 5.0, 60°C Acremonium sp.
3.2.1.168 68.2
-
hesperidin methylchalcone pH 5.0, 60°C Acremonium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.168 5
-
-
Acremonium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.168 4 8 pH 4: ca. 70% relative activity, pH 8: ca. 60% relative activity Acremonium sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.168 Acremonium sp. isoelectric focusing
-
5.7

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.168 7.8
-
hesperidin methylchalcone pH 5.0, 60°C Acremonium sp.
3.2.1.168 18.3
-
hesperidin pH 5.0, 60°C Acremonium sp.