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Literature summary extracted from

  • Asencion Diez, M.D.; Demonte, A.; Giacomelli, J.; Garay, S.; Rodrigues, D.; Hofmann, B.; Hecht, H.J.; Guerrero, S.A.; Iglesias, A.A.
    Functional characterization of GDP-mannose pyrophosphorylase from Leptospira interrogans serovar Copenhageni (2010), Arch. Microbiol., 192, 103-114.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.13 Mg2+
-
Leptospira interrogans serovar Copenhageni

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.13 His-tagged version expressed in Escherichia coli BL21(DE3) Leptospira interrogans serovar Copenhageni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.13 0.063
-
alpha-D-mannose 1-phosphate GDP-mannose synthesis Leptospira interrogans serovar Copenhageni
2.7.7.13 0.089
-
diphosphate pyrophosphorolysis Leptospira interrogans serovar Copenhageni
2.7.7.13 0.202
-
GDP-mannose pyrophosphorolysis Leptospira interrogans serovar Copenhageni
2.7.7.13 0.236
-
GTP GDP-mannose synthesis Leptospira interrogans serovar Copenhageni
2.7.7.13 0.466
-
Mg2+ GDP-mannose synthesis Leptospira interrogans serovar Copenhageni
2.7.7.13 0.466
-
Mg2+ pyrophosphorolysis Leptospira interrogans serovar Copenhageni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.13 45000
-
2 * 45000, His-tagged version, gel filtration, SDS-PAGE Leptospira interrogans serovar Copenhageni

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.13 Leptospira interrogans serovar Copenhageni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.13 affinity chromatography using Talon-Co2+ resin, gel filtration Leptospira interrogans serovar Copenhageni

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.13 3.8
-
-
Leptospira interrogans serovar Copenhageni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
Leptospira interrogans serovar Copenhageni diphosphate + GDP-mannose
-
?
2.7.7.13 GTP + alpha-D-mannose 1-phosphate 60% activity with ITP, 2% activity with UTP, 1% activity with glucose-1-phosphate, and 2% activity with ATP and glucose-1-phosphate Leptospira interrogans serovar Copenhageni diphosphate + GDP-alpha-D-mannose
-
r

Subunits

EC Number Subunits Comment Organism
2.7.7.13 dimer 2 * 45000, His-tagged version, gel filtration, SDS-PAGE Leptospira interrogans serovar Copenhageni

Synonyms

EC Number Synonyms Comment Organism
2.7.7.13 GDP-mannose pyrophosphorylase
-
Leptospira interrogans serovar Copenhageni
2.7.7.13 mannose-1-phosphate guanylyltransferase
-
Leptospira interrogans serovar Copenhageni

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.13 Leptospira interrogans serovar Copenhageni calculated
-
9.35