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Literature summary extracted from

  • Bialkowska, A.M.; Cieslinski, H.; Nowakowska, K.M.; Kur, J.; Turkiewicz, M.
    A new beta-galactosidase with a low temperature optimum isolated from the antarctic Arthrobacter sp. 20B: gene cloning, purification and characterization (2009), Arch. Microbiol., 191, 825-835.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.23 2-mercaptoethanol 53% rise in activity in the presence of 10 mM 2-beta-mercaptoethanol Arthrobacter sp.
3.2.1.23 dithiothreitol 25% rise in activity in the presence of 10 mM dithiothreitol Arthrobacter sp.
3.2.1.23 additional information the enzyme is not stimulated by glutathione Arthrobacter sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.23 expressed in Escherichia coli TOP10F‘ cells Arthrobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 4-chloromercuribenzoic acid complete inhibition at 10 mM Arthrobacter sp.
3.2.1.23 Ca2+ 95% residual activity at 5 mM Arthrobacter sp.
3.2.1.23 Cu2+ complete inhibition at 5 mM Arthrobacter sp.
3.2.1.23 Pb2+ 15% residual activity at 5 mM Arthrobacter sp.
3.2.1.23 Zn2+ 50% residual activity at 5 mM Arthrobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 K+ 49% increase of activity at 5 mM Arthrobacter sp.
3.2.1.23 Mn2+ 11% increase of activity at 5 mM Arthrobacter sp.
3.2.1.23 additional information the enzyme is not affected by Mg2+ Arthrobacter sp.
3.2.1.23 Na+ 53% increase of activity at 5 mM Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 113695
-
 4 * 113695, calculated from amino acid sequence Arthrobacter sp.
3.2.1.23 116000
-
 4 * 116000, native and SDS-PAGE Arthrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.23 lactose + H2O Arthrobacter sp.
-
 D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O Arthrobacter sp. 20B
-
 D-galactose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Arthrobacter sp.
-
-
-
3.2.1.23 Arthrobacter sp. 20B
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23 DEAE-Sepharose column chromatography, PABTG-Sepharose column chromatography, and Sephacryl S-300 gel filtration Arthrobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 0.35
-
 cell extract, in 50 mM potassium phosphate buffer, pH 7.3, containing 100 mM NaCl and 100 mM beta-mercaptoethanol Arthrobacter sp.
3.2.1.23 2.6
-
 after 7fold purification, in 50 mM potassium phosphate buffer, pH 7.3, containing 100 mM NaCl and 100 mM beta-mercaptoethanol Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O 3times lower activity than for 4-nitrophenyl-beta-D-galactopyranoside Arthrobacter sp. 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O 3times lower activity than for 4-nitrophenyl-beta-D-galactopyranoside Arthrobacter sp. 20B 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O preferred substrate, 3times higher activity than for 2-nitrophenyl-beta-D-galactopyranoside Arthrobacter sp. 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O preferred substrate, 3times higher activity than for 2-nitrophenyl-beta-D-galactopyranoside Arthrobacter sp. 20B 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Arthrobacter sp. D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Arthrobacter sp. 20B D-galactose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 homotetramer 4 * 113695, calculated from amino acid sequence Arthrobacter sp.
3.2.1.23 homotetramer 4 * 116000, native and SDS-PAGE Arthrobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 BgaS
-
 Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 25
-
-
 Arthrobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.23
-
 45 the enzyme displays 52-85% maximum activity at 0-20°C, about 50% activity is seen at 45°C Arthrobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23
-
 35 the enzyme remains stable from 0-35°C, above 35°C there is a constant drop of activity with about 60% relative activity at 40°C, 30% relative activity at 45°C, and no activity at 60-70°C Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 6 8
-
 Arthrobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.23 5 9
-
 Arthrobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.23 5.5 8 the enzyme remains stable from pH 5.5-8.0, about 40% relative activity at pH 4.0 and 10.0, about 70% relative activity at pH 5.0 and 9.0, less than 10% activity at pH 11.0 Arthrobacter sp.

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.23 5
-
 at 25°C Arthrobacter sp. Zn2+